The consumption of legumes positively correlated with the reduction body weight. In present study, we identified and evaluated pancreatic lipase inhibitors from Vigna unguiculata unraveled their mode inhibition. highly sensitive fluorometric method was adopted to access activity ethanolic extract showed maximum inhibition (IC50 15.2 µg/ml). Cyanidin cyanidin-3-glucoside are major anthocyanins observed in unguiculata. IC50 value cyanidin 28.29 µM which 6.5-fold higher than (188.28 µM). We...

Abstract Alpha/beta hydrolase domain (ABHD)-containing proteins are structurally related with diverse catalytic activities. In various species, some ABHD have been characterized and shown to play roles in lipid homeostasis. However, little is known about plants. Here, we AT4G10030 (AtABHD11), an Arabidopsis (Arabidopsis thaliana) homolog of a human ABHD11 gene. silico analyses AtABHD11 revealed homology other plant species conserved GXSXG motif. Interestingly, abhd11 mutant plants exhibited...

Abstract Monoacylglycerol acyltransferase (MGAT) catalyzes the synthesis of diacylglycerol, precursor triacylglycerol biosynthesis and an important signaling molecule. Here, we describe isolation characterization peanut (Arachis hypogaea) MGAT gene. The soluble enzyme utilizes invariant histidine-62 aspartate-67 residues motif for its activity. A sequence analysis revealed presence a hydrolase (GXSXG) motif, assays monoacylglycerol (MAG) lysophosphatidylcholine (LPC) hydrolytic activities,...

Elucidating proteolipidome dynamics is crucial for understanding the roles of these molecules in plant physiology and disease. Sequence-based functional annotation protein inadequate, since activities depend on posttranslational modification. In this study, we applied a gel-free activity-based profiling approach to unravel active lipases, including other Serine hydrolases (SHs), expressed during seed germination rice (Oryza sativa). We successfully mapped sites 43 SHs encompassing...

Abstract Rice bran is an underutilized agricultural by-product with economic importance. The unique phytochemicals and fatty acid compositions of have been targeted for nutraceutical development. endogenous lipases hydrolases are responsible the rapid deterioration rice bran. Hence, we attempted to provide first comprehensive profiling active serine (SHs) present in proteome by activity-based protein (ABPP) strategy. site-directed fluorophosphonate probe (rhodamine biotin-conjugated) was...

ABSTRACT Lipopolysaccharide (LPS) is an endotoxin, a potent stimulator of immune response and induction LPS leads to acute lung injury (ALI)/acute respiratory distress syndrome (ARDS). ARDS life‐threatening disease worldwide with high mortality rate. The immunological effect spleen thymus well documented; however the impact on membrane phospholipid during endotoxemia has not yet been studied. Hence we aimed investigate influence fatty acid composition by [ 32 P]orthophosphate labeling in...

Introduction and Aim: The oxidative stability of edible oils is a critical factor influencing their health benefits suitability for various food applications. fatty acid composition significantly impacts the susceptibility to oxidation. Edible play pivotal role in cooking production, making matter paramount importance. Oxidative affects product quality, shelf life, nutritional value, benefits. Accelerated tests have emerged address these challenges more efficiently. Therefore, objective...

Abstract Tobacco products and cigarette smoke cause many respiratory diseases including cancer. 4-(Methyl nitrosamino)-1-(3-pyridyl)-1-butanone (NNK) is a potent carcinogen in smoke, but its effect on lipid metabolism remains enigmatic. Hence, Saccharomyces cerevisiae was exposed to different concentrations of NNK (0–400 µmol L−1) elucidate role metabolism. Exposure substantially decreases (about 60%) the phospholipid content with concomitant increase lysophospholipids. Significant reduction...

Activity-based protein profiling (ABPP) is a chemoproteomics platform to assess the functional state of enzymes in complex biological systems. Over two decades, ABPP has emerged from gel-based gel-free platform, for in-depth proteome analysis with enhanced resolution, sensitivity target detection, and discovery small molecule inhibitors. The format coupled advanced mass spectrometry highly sensitive provides more comprehensive knowledge targeted enzyme family than method. strategy applied...