A5010849619- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- CRISPR and Genetic Engineering
- Cancer-related gene regulation
- Innovation and Socioeconomic Development
- Gene Regulatory Network Analysis
- Neurobiology and Insect Physiology Research
- Climate Change Communication and Perception
- Retinal Development and Disorders
- Animal Behavior and Reproduction
- Lipid metabolism and biosynthesis
- Metabolism, Diabetes, and Cancer
- Cancer-related Molecular Pathways
- Evolution and Genetic Dynamics
- Bioinformatics and Genomic Networks
- Interdisciplinary Research and Collaboration
- Microbial Metabolic Engineering and Bioproduction
- Insect symbiosis and bacterial influences
Stanford University
2014-2017
Washington University in St. Louis
2009-2010
University of Maryland, College Park
2010
Significance Cas9, a protein derived from the bacterial CRISPR/Cas9 immune system, relies on programmable single-guide RNA (sgRNA) to bind specific genomic sequences. Cas9 complexed with sgRNA readily binds on-target DNA, but models that can predict specificity of this process have proven elusive. To investigate system biophysical perspective, we applied massively parallel method for profiling protein–DNA interactions quantify nuclease-dead (dCas9) binding across thousands off-target We...
RNA-binding proteins (RBPs) control the fate of nearly every transcript in a cell. However, no existing approach for studying these posttranscriptional gene regulators combines transcriptome-wide throughput and biophysical precision. Here, we describe an assay that accomplishes this. Using commonly available hardware, built customizable, open-source platform leverages inherent Illumina technology direct measurements. We used to quantitatively measure binding affinity prototypical RBP Vts1...
Visual sensitivity can be tuned by differential expression of opsin genes. Among African cichlid fishes, seven cone genes are expressed in different combinations to produce diverse visual sensitivities. To determine the genetic architecture controlling these adaptive differences, we analysed crosses between species expressing complements Quantitative analyses suggest that is controlled only a few loci with correlations among some Genetic mapping identifies clear evidence trans-acting factors...
The bacterial adaptive immune system CRISPR-Cas9 has been appropriated as a versatile tool for editing genomes, controlling gene expression, and visualizing genetic loci 1 .To analyze Cas9's ability to bind DNA rapidly specifically, we measured the kinetics of catalytically dead Cas9 (dCas9) interactions with library potential binding partners.Using massively parallel assay protein-DNA derived from high-throughput sequencing flow cell (HiTS-FLIP) 2 building on established importance...