A5078645646- Dialysis and Renal Disease Management
- Heat shock proteins research
- Ubiquitin and proteasome pathways
- Neuroscience and Neuropharmacology Research
- Genetics and Neurodevelopmental Disorders
- Endoplasmic Reticulum Stress and Disease
- Memory and Neural Mechanisms
- Retinal Development and Disorders
- Chronic Kidney Disease and Diabetes
- Muscle and Compartmental Disorders
- Advanced Glycation End Products research
- Vitamin C and Antioxidants Research
- S100 Proteins and Annexins
- CAR-T cell therapy research
- Immunotherapy and Immune Responses
- Blood Pressure and Hypertension Studies
- Cellular transport and secretion
- Ovarian cancer diagnosis and treatment
- Bipolar Disorder and Treatment
- Hepatitis C virus research
- Nicotinic Acetylcholine Receptors Study
- Phosphodiesterase function and regulation
- Antioxidant Activity and Oxidative Stress
- Receptor Mechanisms and Signaling
- Neural dynamics and brain function
Otto-von-Guericke University Magdeburg
2015-2022
Leibniz Institute for Neurobiology
2011-2013
The immunodominant MART-1(26(27)-35) epitope, liberated from the differentiation antigen melanoma recognized by T cells/melanoma A (MART-1/Melan-A), has been frequently targeted in immunotherapy, but with limited clinical success. Previous studies suggested that this is part due to an insufficient peptide supply and epitope presentation, since proteasomes containing immunosubunits β5i/LMP7 (LMP, low molecular weight protein) or β1i/LMP2 interfere MART-1(26-35) generation tumor cells. Here,...
The A-kinase-anchoring protein 5 (AKAP5), a post-synaptic multi-adaptor molecule that binds G-protein-coupled receptors and intracellular signaling molecules has been implicated in emotional processing rodents, but its role human emotion behavior is up to now still not quite clear. Here, we report an association of individual differences aggressive anger expression with functional genetic polymorphism (Pro100Leu) the AKAP5 gene. Among cohort 527 young, healthy individuals, carriers less...
Hemodialysis (HD) patients exhibit chronic inflammation and leukocyte activation. We investigated the surface-marker profile of monocytes by flow cytometry to assess effect uremia acute dialysis on their phenotypical functional features in 16 healthy controls (CON) 15 HD before after a polysulfone-based session. Median fluorescence intensities were analyzed indicating expression CD14, CD16, integrins (CD11b, CD18), chemokine receptors (CCR2, CX3CR1), scavenger (CD36, CD163) Toll-like...
The A kinase-anchoring protein AKAP79/150 is a postsynaptic scaffold molecule and key regulator of signaling events. At the postsynapse it coordinates phosphorylation dephosphorylation receptors via anchoring kinases phosphatases near their substrates. Interactions between AKAP79 two Ca(2+) -binding proteins caldendrin calmodulin have been investigated here. Calmodulin known interaction partner that has shown to regulate activity kinase PKC in -dependent manner. Pull-down experiments surface...
Recent investigations addressing the role of synaptic multiadaptor molecule AKAP5 in human emotion and behavior suggest that Pro100Leu polymorphism (rs2230491) contributes to individual differences affective control. Carriers less common Leu allele show a higher control anger as indicated by behavioral measures dACC brain response on emotional distracters when compared Pro homozygotes. In current fMRI study we used an working memory task according n-back scheme with neutral negative faces...
Cold shock proteins are up-regulated by cellular stress and orchestrate inflammatory responses, cell proliferation, differentiation. Enhanced cold protein expression promotes malignant transformation; up-regulation is detected in most cancers associated with poor prognosis. Akt1, a serine/threonine kinase, potent oncogene, which activates pro-proliferative anti-apoptotic signaling pathways, phosphorylates the domain. Unexpectedly, chicken-YB-1 abrogates PI3K-Akt-dependent oncogenic...
The kinetic and mechanistic details of the interaction between caldendrin, calmodulin B-domain AKAP79 were determined using a biosensor-based approach. Caldendrin was found to compete with for binding at AKAP79, indicating overlapping sites. Although affinities similar caldendrin (K(D) = 20 nM) 30 nM), their characteristics different. well described by reversible one-step model, but only detected in presence Ca(2+). interacted higher level complexity, deduced be an induced fit mechanism slow...
Abstract The major causes for increased morbidity and mortality among chronic kidney disease patients are cardiovascular diseases infection. A causal link between an activated immune system aggravated atherosclerosis has been postulated that skews the towards inflammatory responses. Previously, we demonstrated a positive association of pro-inflammatory cytokines with monocytic Y-box binding protein-1 (YB-1) expression vessel wall infiltration in hemodialysis patients. Here, question whether...
Proteolysis as mediated by one of the major cellular protein degradation pathways, ubiquitin-proteasome system (UPS), plays an essential role in learning and memory formation. However, functional relevance immunoproteasomes healthy brain especially their impact on normal function including processes has not been investigated so far. In present study, we analyzed phenotypic effects impaired immunoproteasome formation using a β5i/LMP7-deficient mouse model different behavioral paradigms...
By changing the relative abundance of generated antigenic peptides through alterations in proteolytic activity, interferon (IFN)-γ-induced immunoproteasomes influence outcome CD8+ cytotoxic T lymphocyte responses. In present study, we investigated effects hepatitis C virus (HCV) infection on IFN-γ-induced immunoproteasome expression using a HCV cell culture system. We found that, although IFN-γ induced transcriptional mRNAs encoding β1i/LMP2, β2i/MECL-1 and β5i/LMP7 subunits, formation was...
Introduction: The cold shock Y-box binding protein-1 (YB-1) fulfills important roles in regulating cell proliferation and differentiation. Overexpression occurs various tumor cells. Given the existence of extracellular YB-1 we set out to determine diagnostic, predictive prognostic role serum YB-1/p18 for patients with primary epithelial ovarian cancer (EOC).
In end-stage renal disease, a high cardiovascular risk profile and endothelial damage prevails. The heparin-binding growth factor midkine stimulates neo-angiogenesis in ischemic diseases, coordinates neutrophil influx, raises blood pressure through stimulated angiotensin synthesis.We determined changes of serum levels during hemodialysis sessions under the assumption that cell-derived is released. Periprocedural differences (∆midkine) were calculated correlated with cardiovacular biomarkers...