A5081725440- interferon and immune responses
- Myofascial pain diagnosis and treatment
- Musculoskeletal pain and rehabilitation
- Immune Response and Inflammation
- CRISPR and Genetic Engineering
- Inflammasome and immune disorders
- Protein Structure and Dynamics
- Acupuncture Treatment Research Studies
- Sports injuries and prevention
- RNA and protein synthesis mechanisms
- Mosquito-borne diseases and control
- Microbial Metabolic Engineering and Bioproduction
- Influenza Virus Research Studies
- Bacterial Genetics and Biotechnology
- Cytomegalovirus and herpesvirus research
- Enzyme Structure and Function
- Gastrointestinal Tumor Research and Treatment
- Coronary Interventions and Diagnostics
- Cancer Immunotherapy and Biomarkers
- RNA regulation and disease
- Sports Performance and Training
- Cancer-related molecular mechanisms research
- Pluripotent Stem Cells Research
- Immune cells in cancer
- Cancer survivorship and care
University Hospital Bonn
2014-2024
MSH Medical School Hamburg – University of Applied Sciences and Medical University
2022-2024
Roche (Switzerland)
2018-2024
Lund University
2024
Universitätsmedizin Göttingen
2019-2021
Universität Hamburg
2010-2020
GlaxoSmithKline (United Kingdom)
2019
University of Göttingen
1993-2019
Heidelberg University
2014-2018
University Hospital Heidelberg
2014-2018
Protein structure homology modelling has become a routine technique to generate 3D models for proteins when experimental structures are not available. Fully automated servers such as SWISS-MODEL with user-friendly web interfaces reliable without the need complex software packages or downloading large databases. Here, we describe latest version of expert system protein modelling. The template library provides annotation quaternary and essential ligands co-factors allow building complete...
Inflammasomes are high molecular weight protein complexes that assemble in the cytosol upon pathogen encounter. This results caspase-1-dependent pro-inflammatory cytokine maturation, as well a special type of cell death, known pyroptosis. The Nlrp3 inflammasome plays pivotal role defense, but at same time, its activity has also been implicated many common sterile inflammatory conditions. To this effect, several studies have identified engagement number human diseases such atherosclerosis, 2...
Organic cation transporters (OCTs) can mediate metformin transmembrane transport. We explored pharmacokinetics in relation to genetic variations OCT1, OCT2, OCT3, OCTN1, and MATE1 103 healthy male Caucasians. Renal clearance varied 3.8-fold was significantly dependent on creatinine (r2 = 0.42, P < 0.0001), age 0.09, 0.002), OCT1 polymorphisms. Carriers of zero, one, two low-activity alleles (Arg61Cys, Gly401Ser, 420del, or Gly465Arg) had mean renal clearances 30.6, 33.1, 37.1 l/h,...
Inflammasome activation culminates in of caspase‐1, which leads to the maturation and subsequent release cytokines interleukin 1 (IL‐1) family results a particular form cell death known as pyroptosis. In addition, murine system, so‐called non‐canonical inflammasome involving caspase‐11 has been described that directly responds cytosolic LPS. Here, we show human monocytic line THP1 activates response LPS TLR4‐independent fashion. This is mediated by caspase‐4 accompanied caspase‐1 activation,...
The Protein Model Portal (PMP) has been developed to foster effective use of 3D molecular models in biomedical research by providing convenient and comprehensive access structural information for proteins. Both experimental structures theoretical a given protein can be searched simultaneously analyzed variability. By view on information, PMP offers the opportunity apply consistent assessment validation criteria complete set available is an open project so that new methods community...
Abstract Inflammation promotes phenotypic plasticity in melanoma, a source of non-genetic heterogeneity, but the molecular framework is poorly understood. Here we use functional genomic approaches and identify reciprocal antagonism between melanocyte lineage transcription factor MITF c-Jun, which interconnects inflammation-induced dedifferentiation with pro-inflammatory cytokine responsiveness melanoma cells favouring myeloid cell recruitment. We show that cytokines such as TNF-α instigate...
Abstract In the Ninth Edition of Critical Assessment Techniques for Protein Structure Prediction (CASP9), 61,665 models submitted by 176 groups were assessed their accuracy in template based modeling category. The evaluated numerically comparison to experimental control structures using two global measures (GDT and GDC), a novel local score evaluating correct interactions (lDDT). Overall, state art CASP9 is high, with many performing well. Among methods registered as prediction “servers”,...
The translational potential of cell-based therapies is often limited by complications related to effectively engineering and manufacturing functional cells. While the use electroporation widespread, impact on cell state function has yet be fully characterized. Here, we a genome-wide approach study optimized treatment identify striking disruptions in expression profiles key transcripts human T These genetic result concomitant perturbation cytokine secretion including 648-fold increase IL-2 (P...
Human cytomegalovirus (HCMV) infections of healthy individuals are mostly unnoticed and result in viral latency. However, HCMV can also cause devastating disease, e.g., upon reactivation immunocompromised patients. Yet, little is known about human immune cell sensing DNA-encoded HCMV. Recent studies indicated that during infection the cyclic GMP/AMP synthase (cGAS) senses cytosolic DNA catalyzes formation di-nucleotide cGAMP, which triggers stimulator interferon genes (STING) thus induces...
The application of designer nucleases allows the induction DNA double-strand breaks (DSBs) at user-defined genomic loci. Due to imperfect repair mechanisms, DSBs can lead alterations in architecture, such as disruption reading frame a critical exon. This be exploited generate somatic knockout cell lines. While high genome editing activities achieved various cellular systems, obtaining clones that contain all-allelic frameshift mutations target locus interest remains laborious task. To this...
Research projects in structural biology increasingly rely on combinations of heterogeneous sources information, e.g. evolutionary information from multiple sequence alignments, experimental evidence the form density maps and proximity constraints proteomics experiments. The OpenStructure software framework, which allows seamless integration different origin, has previously been introduced. consists C++ libraries are fully accessible Python programming language. Additionally, framework...
(1) Background: Inflammatory processes in the thoracolumbar fascia (TLF) lead to thickening, compaction, and fibrosis are thought contribute development of nonspecific low back pain (nLBP). The blood flow (BF) fascial tissue may play a critical role this process, as it promote hypoxia-induced inflammation. primary objective study was examine immediate effects set myofascial release (MFR) techniques on BF lumbar tissue. secondary objectives were evaluate influence TLF morphology (TLFM),...
Abstract Interactions between proteins and their ligands play central roles in many physiological processes. The structural details for most of these interactions, however, have not yet been characterized experientially. Therefore, various computational tools developed to predict the location binding sites amino acid residues interacting with ligands. In this manuscript, we assess performance 33 methods participating ligand‐binding site prediction category CASP9. overall accuracy predictions...
Physical exercise has been linked to higher cognitive functioning and enhanced brain plasticity in aging humans. The most consistent positive effects have reported for executive functions associated with frontal regions. In rodents, however, running shown induce functional structural changes the hippocampus, a region known be important memory. It is still matter of debate which are susceptible whether an increase cardiovascular fitness beneficial functioning. Moreover, little about impact on...
Report22 December 2016Open Access Transparent process Polysialic acid blocks mononuclear phagocyte reactivity, inhibits complement activation, and protects from vascular damage in the retina Marcus Karlstetter Laboratory for Experimental Immunology of Eye, Department Ophthalmology, University Cologne, Germany Therapeutic Research Group Bayer Pharma AG, Wuppertal, Search more papers by this author Jens Kopatz Institute Reconstructive Neurobiology, Hospital Bonn, Alexander Aslanidis Anahita...