A5100318800- Invertebrate Immune Response Mechanisms
- Neurobiology and Insect Physiology Research
- Insect Utilization and Effects
- Ubiquitin and proteasome pathways
- Antimicrobial Peptides and Activities
- Peptidase Inhibition and Analysis
- Aquaculture disease management and microbiota
- SARS-CoV-2 and COVID-19 Research
- Cancer Research and Treatments
- Insect Resistance and Genetics
- Cancer, Hypoxia, and Metabolism
- Skin Protection and Aging
- Monoclonal and Polyclonal Antibodies Research
- Wound Healing and Treatments
- Insect and Arachnid Ecology and Behavior
- Insect symbiosis and bacterial influences
- Cancer Immunotherapy and Biomarkers
- Erythrocyte Function and Pathophysiology
- Toxic Organic Pollutants Impact
- Proteoglycans and glycosaminoglycans research
- Adipose Tissue and Metabolism
- Seaweed-derived Bioactive Compounds
- Tuberculosis Research and Epidemiology
- Insect and Pesticide Research
- Hepatitis B Virus Studies
CIC bioGUNE
2013-2025
Gachon University Gil Medical Center
2018-2024
Korea Food Research Institute
2020-2024
Korea University of Science and Technology
2024
National University of Singapore
2023
Asan Medical Center
2005-2022
University of Ulsan
2000-2022
Ulsan College
2005-2022
Genome and Company (South Korea)
2022
Kyung Hee University
2022
A lipopolysaccharide- and β-1,3-glucan-binding protein (LGBP) was isolated characterized from blood cells (hemocytes) of the freshwater crayfish Pacifastacus leniusculus. The LGBP purified by chromatography on Blue-Sepharose phenyl-Sepharose, followed Sephacryl S-200. has a molecular mass 36 kDa 40 10% SDS-polyacrylamide gel electrophoresis under reducing nonreducing conditions, respectively. calculated is 39,492 Da, which corresponds to native size LGBP; estimated pI mature 5.80. binding...
An antibacterial peptide with 16 amino acid residues was found in plasma of the freshwater crayfish, Pacifastacus leniusculus. This peptide, designated astacidin 1, purified by cation-exchange column chromatography and reverse-phase high performance liquid chromatography. Astacidin 1 has a broad range activity, it inhibits growth both Gram-positive Gram-negative bacteria. The primary sequence FKVQNQHGQVVKIFHH-COOH. molecular mass 1945.2 Da, no carbohydrate-linked could be spectrometry. A...
Abstract Hemopoietic development requires firm control of cell proliferation and differentiation. Although recent research has revealed conserved function transcription factors signaling pathways regulating lineage commitment in hemopoietic Drosophila melanogaster vertebrates, little is known about cytokines among the invertebrate phyla. In present study, we show that differentiation growth stem cells vitro from an invertebrate, Pacifastacus leniusculus, require endogenous cytokine-like...
Berberine (BBR), an isoquinoline alkaloid, has a wide range of pharmacological effects, yet its exact mechanism is unknown. In order to understand the anti-adipogenic effect BBR, we studied change expression several adipogenic enzymes 3T3-L1 cells by BBR treatment. First, measured leptin and glycerol in medium treated with 1 micrometer, 5 micrometer 10 concentrations BBR. We also changes lipolytic factors 3T3-L1. cells, both (SREBP-1c, C/EBP-alpha, PPAR-gamma, fatty acid synthase, acetyl-CoA...
Increased cancer stem cell content during development of resistance to tamoxifen in breast is driven by multiple signals, including Sox2-dependent activation Wnt signalling. Here, we show that Sox2 increases and estrogen reduces the expression transcription factor Sox9. Gain loss function assays indicate Sox9 implicated maintenance human luminal progenitor cells. CRISPR/Cas knockout growth tamoxifen-resistant tumours vivo. Mechanistically, acts downstream control required for marker ALDH1A3...
The tumor microenvironment plays a key role in cancer development and progression is involved resistance to chemo- immunotherapy. Cancer-associated fibroblast expressing fibroblast-activating protein α (FAPα) one of the predominant stroma cell types immunotherapy.We generated OMTX705, novel antibody-drug conjugate from humanized anti-FAP antibody linked new cytolysin. Here, we studied its antineoplastic activity vitro preclinical mouse models alone combination with chemotherapy as well...
The prophenoloxidase activating enzyme (ppA), a serine proteinase catalyzing the conversion of to an active phenoloxidase, has molecular mass about 36 kDa in its form. This protein was cloned from blood cell cDNA library and corresponding 1736 base pairs encodes zymogenic (proppA) 468 amino acids. An antibody raised against synthetic peptide derived region sequence could efficiently inhibit β‐1,3‐glucan triggered activation vitro. C‐terminal half proppA is composed typical domain, with...
Abstract A multifunctional masquerade-like protein has been isolated, purified, and characterized from hemocytes of the freshwater crayfish, Pacifastacus leniusculus. It was isolated by its Escherichia coli binding property, it binds to formaldehyde-treated Gram-negative bacteria as well yeast, Saccharomyces cerevisiae, whereas does not bind formaldehyde-fixed Gram-positive bacteria. The intact masquerade (mas)-like is present in crayfish a heterodimer composed two subunits with molecular...
The prophenoloxidase (proPO) activation pathway, like the vertebrate complement system, consists of a protease cascade and functions as non-self-recognition system in these animals. Determining molecular mechanism by which pattern recognition molecules differentiate non-self from self transduce signals that stimulate defense responses is key for understanding ways innate immune systems are regulated. However, proPO poorly defined at level. proPO-activating insect Holotrichia diomphalia...
Members of the transforming growth factor β (TGF-β) and Wnt/wingless superfamilies regulate cell fate during development tissue maintenance. Here we report that Smad7 interacts with β-catenin lymphoid enhancer binding 1/T-cell-specific (LEF1/TCF), transcriptional regulators in Wnt signaling, a TGF-β-dependent manner. was found to be required for TGF-β1-induced accumulation LEF1 human prostate cancer (PC-3U) cells as well keratinocytes (HaCaT cells). Moreover, when endogenous repressed by...
Background Diabetes mellitus is a risk factor for tuberculosis (TB) disease. There evidence that diabetes also influences TB severity and treatment outcomes but information incomplete some published results have been inconsistent. Methods A longitudinal cohort study was conducted at the National Masan Tuberculosis Hospital in Republic of Korea. Subjects presenting with first episode or retreatment were followed from enrollment through completion treatment. Demographic, clinical,...
Jujuboside B (1) is one of the saponins isolated from seeds Zizyphus jujuba var. spinosa, which are used as a well-known traditional medicine for treatment insomnia and anxiety in East Asian countries. This first study to investigate antitumor mechanism 1 vivo vitro. The results showed that induced apoptosis autophagy AGS HCT 116 human cancer cells also effectively suppressed tumor growth nude mouse xenograft model bearing cells. apoptosis-inducing effect was characterized by annexin...
Previously, we purified and characterized a pro-phenol-oxidase (pro-PO) of 79 kDa from coleopteran insect, Holotrichia diomphalia larvae [Kwon et al. (1997) Mol. Cells 7, 90-97]. Here, describe the identification two pro-PO-activating factors (PPAF), named PPAF-I PPAF-II, directly involved in activation isolated pro-PO. When pro-PO was incubated with either or no phenol oxidase activity observed. However, incubation both PPAF-II specifically exhibited activity. The molecular mass 33 on...
Previously, we identified two pro‐phenol oxidase‐activating factors, named PPAF‐I and PPAF‐II, directly involved in the activation of purified oxidase (pro‐PO) from hemolymph coleopteran, Holotrichia diomphalia larvae [Lee, S. Y., Kwon, T. H., Hyun, J. Choi, S., Kawabata, I., Iwanga, S, & Lee, B. L. (1998) Eur. Biochem . 254 , 90−97]. Here, report molecular cloning cDNA for PPAF‐I. Based on sequence cloned cDNA, gene appears to encode a member serine protease zymogen consisting 365 amino...
Antibacterial activity was induced in the hemolymph of larvae coleopteran Tenebrio molitor by injection Escherichia coli* An antibacterial protein, named tenecin 1, purified to homogeneity from larval and characterized. A cDNA clone for 1 isolated its complete sequence determined. This protein found inhibit growth Gram-positive bacteria consist 43-amino acid residues including six cysteine residues. The disulfide structure determined sequencing containing peptides obtained digesting with...
Abstract Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causes a multi-organ damage that includes hepatic dysfunction, which has been observed in over 50% of COVID-19 patients. Liver injury could be attributed to the cytopathic effects, exacerbated immune responses or treatment-associated drug toxicity. Herein we demonstrate hepatocytes are susceptible infection different models: primary derived from humanized angiotensin-converting enzyme-2 mice (hACE2) and human hepatocytes....