A5015750622- HIV Research and Treatment
- Monoclonal and Polyclonal Antibodies Research
- HIV/AIDS drug development and treatment
- RNA and protein synthesis mechanisms
- Lipid Membrane Structure and Behavior
- Esophageal and GI Pathology
- Bariatric Surgery and Outcomes
- Gastroesophageal reflux and treatments
- Glycosylation and Glycoproteins Research
- Immune Cell Function and Interaction
Brown University
2024
Miriam Hospital
2024
National Institutes of Health
2018-2022
National Institute of Allergy and Infectious Diseases
2018-2022
Structural and functional studies of HIV envelope glycoprotein (Env) as a transmembrane protein have long been complicated by challenges associated with inherent flexibility the molecule membrane-embedded hydrophobic regions. Here, we present approaches for incorporating full-length, wild-type HIV-1 Env, well C-terminally truncated stabilized versions, into lipid assemblies, providing modular platform Env structural single particle electron microscopy. We reconstitute full-length clone...
The developmental pathways of broadly neutralizing antibodies (bNAbs) against HIV are great importance for the design immunogens that can elicit protective responses. Here we show maturation features HIV-neutralizing anti-V1V2 VRC26 lineage by simultaneously sequencing exon together with downstream intron members. Using mutational landscapes both segments and selection-free nature region, identify multiple events amino acid convergence in complementarity-determining region 3 (CDR3) members,...
The broadly neutralizing antibody (bNAb) CAP256-VRC26.25 has exceptional potency against HIV-1 and been considered for clinical use. During the characterization production of this bNAb, we observed several unusual features. First, appeared to adhere pipette tips, requiring tips be changed during serial dilution accurately measure potency. Second, scale-up, proteolytic cleavage was discovered target an extended heavy chain loop, which attributed a protease in spent medium from 2-week culture....
Summary Structural and functional studies of HIV Env as a transmembrane protein have long been complicated by challenges associated with inherent flexibility the molecule membrane-embedded hydrophobic regions. Thus, most structural utilized soluble forms where regions C-terminal to ectodomain are deleted. Here, we present approaches for incorporating full-length, wild-type HIV-1 Env, well C-terminally truncated stabilized versions, into lipid assemblies, providing modular platform single...
Abstract The broadly neutralizing antibody (bNAb) CAP256-VRC26.25 has exceptional potency against HIV-1 and been considered for clinical use. During the characterization production of this bNAb, we observed several unusual features. First, appeared to adhere pipette tips, requiring tips be changed during serial dilution accurately measure potency. Second, scale-up, proteolytic cleavage was discovered target an extended heavy chain loop, which attributed a protease in spent medium from...