A5077732823- Genomics and Chromatin Dynamics
- Cancer-related gene regulation
- DNA and Nucleic Acid Chemistry
- Epigenetics and DNA Methylation
- RNA modifications and cancer
- RNA Research and Splicing
University of Manitoba
2022-2025
Doxorubicin is a chemotherapeutic drug for cancer that intercalates into nucleosome-free regions at promoters. was reported to result in loss of histone H3 trimethylated lysine 4 (H3K4me3). To further explore doxorubicin’s mechanism action, we determined the genomic location binding sites doxorubicin leukemic cells. The effect intercalation chromatin cells on modifications also determined. We show were present associated with regulatory (promoters, enhancers and super-enhancers) gene body...
A subset of expressed genes is associated with a broad H3K4me3 (histone H3 trimethylated at lysine 4) domain that extends throughout the gene body. Genes marked in this way normal cells are involved cell-identity and tumor-suppressor activities, whereas cancer cells, driving phenotype (oncogenes) have feature. Other histone modifications display been less studied. Here, we identified broadest H3K79me2 dimethylated 79) human leukemic cell lines representing different forms leukemia. Taking...