A5087152602- 3D Printing in Biomedical Research
- Microfluidic and Bio-sensing Technologies
- Cancer Cells and Metastasis
- Pluripotent Stem Cells Research
- Single-cell and spatial transcriptomics
- Drug Solubulity and Delivery Systems
- Copper-based nanomaterials and applications
- Electrochemical Analysis and Applications
- Biosensors and Analytical Detection
- Cellular Mechanics and Interactions
- Advanced Drug Delivery Systems
- Electrochemical sensors and biosensors
- CRISPR and Genetic Engineering
- Drug Transport and Resistance Mechanisms
- Digestive system and related health
University of North Carolina at Chapel Hill
2017-2022
Communities In Schools of Orange County
2020
North Carolina State University
2019
Bradley University
2017
Three-dimensional organoid culture has fundamentally changed the in vitro study of intestinal biology enabling novel assays; however, its use is limited because an inaccessible luminal compartment and challenges to data gathering a three-dimensional hydrogel matrix. Long-lived, self-renewing 2-dimensional (2-D) tissue cultured from primary colon cells not been accomplished.The surface matrix chemical factors that sustain 2-D mouse colonic human rectal epithelial cell monolayers with...
Animal models are frequently used for in vitro physiologic and drug transport studies of the colon, but there exists significant pressure to improve assay throughput as well achieve tighter control experimental variables than can be achieved with animals. Thus, development a primary colonic epithelium cultured high resistance protein expression functional behavior similar that native would enormous value pharmaceutical research. A collagen scaffold, which degree cross-linking was present...
Surface adsorption of a homologous series pyridine carboxylic acids on hydrated colloidal cerium dioxide (ceria) film is characterized using the combination experimental and computationally determined infrared (IR) spectra. Experimental analyses employ attenuated total reflectance (ATR) IR spectroscopy deposited ceria thin films equilibrated with three at pH 3.0, 5.5, 8.5. The corresponding computational spectra for energy-minimized intermediate base forms use density functional theory...
A simple planar platform that recapitulates the compartmentalization and physiological responses of mouse colon epithelium is presented.
Human induced pluripotent stem cells (hiPSCs) are widely used for disease modeling, tissue engineering, and clinical applications. Although the development of new disease-relevant or customized hiPSC lines is high importance, current automated isolation technologies rely largely on fluorescent labeling cells, thus limiting cell line from many The objective this research was to develop a platform high-throughput cytometry splitting that utilized label-free sensing approach. An image analysis...
A magnetic, microwell-based plate was established enabling automated collection of colony biopsy fragments into known locations for immunofluorescence assays.
Summary The human transcription factors OCT4, SOX2, and NANOG form a core signaling network critical for maintaining stem cell pluripotency self-renewal potential. spatiotemporal expression dynamics of these throughout differentiation is unclear, limiting our understanding fate decisions. Here, we combined CRISPR/Cas9-mediated gene editing with microraft array technology to generate embryonic lines endogenously tagged fluorophores NANOG. Fluorescence time-lapse imaging revealed that...