A5070290517- Amoebic Infections and Treatments
- Parasitic Infections and Diagnostics
- Pancreatitis Pathology and Treatment
- Diagnosis and treatment of tuberculosis
- Immunodeficiency and Autoimmune Disorders
- Immune Response and Inflammation
- Invertebrate Immune Response Mechanisms
- Aquaculture disease management and microbiota
- Reproductive System and Pregnancy
- Aquaculture Nutrition and Growth
- Immune Cell Function and Interaction
- Neonatal Health and Biochemistry
- Immune cells in cancer
- Insect symbiosis and bacterial influences
- Food Allergy and Anaphylaxis Research
- T-cell and B-cell Immunology
- Plant nutrient uptake and metabolism
- Mechanics and Biomechanics Studies
- Bacterial Infections and Vaccines
- Motor Control and Adaptation
- Infections and bacterial resistance
- Paraoxonase enzyme and polymorphisms
- Biliary and Gastrointestinal Fistulas
- Diabetes and associated disorders
- Child Nutrition and Water Access
Universidad Autónoma de Bucaramanga
2024
Instituto Politécnico Nacional
1980-2023
University of New Haven
2016-2023
Escuela Superior Politecnica del Litoral
2003-2006
San Juan City Hospital
2006
Ponce Health Sciences University
2006
Universidad Nacional Autónoma de México
1997
Yale University
1997
Center for Research and Advanced Studies of the National Polytechnic Institute
1989-1993
Universitätsklinikum Erlangen
1989
Culture fluids of peritoneal exudate cells rich in macrophages stimulated DNA synthesis thymocytes and, to lesser extent, spleen cells. We also investigated the effects culture from on vitro response a hapten-carrier protein (fluorescein-menocyanin) using immune mice. Macrophage contained an activity that increased plaque-forming cell both IgG and IgM class. This increase was observed absence any added hapten culture. The helper function T lymphocytes (as evidenced by challenging with...
Cultures of peritoneal exudate cells rich in macrophages were studied for the secretion lymphostimulatory molecules. Two conditions produced increased secretion: (a) addition to cultures various agents that readily interacted with macrophages, such as latex particles, antibody-coated red cells, endotoxin, Listeria organisms, or Be salt; (b) activated lymphocytes. In first case activity was found during 24 48 h after uptake stimuli. Increased normal peptone-stimulated but not injection...
The present work was designed to investigate the capacity of trophozoites Entamoeba histolytica adhere and digest human collagen types I III in vitro. time-course binding ameba both similar. However, kinetics detachment were different for each type. Trophozoites E. cultured on heat-reconstituted type gels produced a well-defined area lysis. Quantitative studies using 14C-labeled revealed that after 24 h incubation, digested three half times more than collagen, thus suggesting presence...
DAO Diseases of Aquatic Organisms Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout JournalEditorsSpecials 32:151-155 (1998) - doi:10.3354/dao032151 Experimental Vibrio harveyi infections in Penaeus vannamei larvae P. A. W. Robertson1, J. Calderon2, L. Carrera2,*, R. Stark1, M. Zherdmant2, B. Austin1,** 1Heriot-Watt University, Riccarton, Edinburgh EH14 4AS, Scotland, UK2Centro Nacional de Acuicultura e Investigaciones...
Polyspecific antibodies bound to Entamoeba induced surface redistribution of membrane components toward the uroid region. Capping antigens was obtained with a single layer in E. histolytica and invadens. This segregation progressed large accumulation folded plasma that extruded as defined vesicular cap. A spontaneous release cap at end capping process took place. These released caps contained most originally whole cell surface. Two-thirds radiolabeled were into medium 2 h. Successive by...
Mortalities of cultured shrimp, Penaeus vannamei (Boone), induced by white spot syndrome virus (WSSV) have occurred in Ecuador since May 1999. Three epidemiological surveys Ecuadorian farms were carried out and showed an apparent association between lower temperature increased mortality rates commercial ponds. Infected animals a reddish discolouration lethargy occasionally, spots the exoskeleton. Histopathological studies revealed that infected cells presented nuclear hypertrophy with...
Purification of the human interferon-gamma (IFN-gamma) receptor was facilitated by identification placenta as a large-scale source. When analyzed in radioligand binding experiments, intact placental membranes and detergent-solubilized membrane proteins expressed 1.3 5.9 X 10(12) receptors per mg protein, respectively, values that were 13-163 times greater than observed for U937 membranes. Two protocols followed to purify IFN-gamma from octyl glucoside-solubilized membranes: (i) sequential...
Abstract Purified preparations of the human IFN-gamma R derived from placental membranes were used to produce receptor-specific murine mAb. Supernatants growth-positive wells screened for their ability block binding 125I-IFN-gamma membranes. Ten inhibitory cultures identified. Two these (GIR-208 and GIR-301) abrogated all radioligand either intact or soluble, purified R. Three others (GIR-72, 76 94) showed moderate blocking activity (65, 59, 49%, respectively) whereas remaining five (GIR-57,...
Abstract Larvae and post‐larvae of Penaeus vannamei (Boone) were submitted to primary challenge with infectious hypodermal haematopoietic necrosis virus (IHHNV) or formalin‐inactivated white spot syndrome (WSSV). Survival rate viral load evaluated after secondary per os WSSV at post‐larval stage 45 (PL45). Only shrimp treated inactivated PL35 IHHNV infection nauplius 5, zoea 1 PL22 alive (4.7% 4%, respectively) 10 days post‐infection (p.i.). Moreover, 9 p.i. there was 100% mortality in all...
Entamoeba histolytica HM1 supported the activation of human alternative and classical complement pathways in absence ameba-reactive antibodies. Nonimmune serum depleted C1q factor D (NHS s + D) reconstituted with was able to specifically deposit C3b onto trophozoites produce lysis. This activity not modified by absorption on E. histolytica. Serum B allowed binding amebae. devoid C4 effected only small amounts C3 uptake. The kinetics lysis presence Mg-EGTA [ethylene glycol-bis(beta-aminoethyl...
Polyspecific antibodies bound to the cell surface of Entamoeba histolytica are polarized toward uroid region and spontaneously released by amoeba as supramolecular aggregate or cap. We purified these exfoliated structures analyzed them electron microscopy sodium dodecyl sulfate electrophoresis. Isolated caps were mainly composed membranes contained five major [35S]methionine-labeled bands; many more bands appeared (together with immunoglobulins) after silver staining. Surface...
Rhizobium etli mutants unable to grow on asparagine as the nitrogen and carbon source were isolated. Two kinds of obtained: AHZ1, with very low levels aspartase activity, AHZ7, asparaginase compared wild-type strain. R. had two asparaginases differentiated by their thermostabilities, electrophoretic mobilities, modes regulation. The AHZ nodulated did strain nitrogenase similar that
The serine-rich Entamoeba histolytica protein (SREHP) has been shown to be a protective antigen in animal models of amebic liver abscess when delivered by either parenteral or oral routes immunization, and antibodies SREHP can prevent severe combined immunodeficient mice. To identify B cell epitopes the molecule that could serve as basis for peptide-based vaccine, we synthesized overlapping peptides spanning amino acid sequence SREHP, looked at reactivity serum samples from five individuals...