A5102005600- Connexins and lens biology
- MicroRNA in disease regulation
- Toxoplasma gondii Research Studies
- Cancer-related molecular mechanisms research
- Advanced Glycation End Products research
- Biochemical effects in animals
- Selenium in Biological Systems
- Heat shock proteins research
- Ubiquitin and proteasome pathways
- Neurological Disease Mechanisms and Treatments
- Retinal Diseases and Treatments
- Cancer-related Molecular Pathways
- Redox biology and oxidative stress
Fourth Affiliated Hospital of China Medical University
2018-2020
China Medical University
2015-2020
MicroRNA-24 (miR-24) serves an important role in cell proliferation, migration and inflammation various types of disease. In the present study, biological function molecular mechanism miR‑24 was investigated association with progression age‑associated cataracts. To best our knowledge study is first to report that expression significantly increased human anterior lens capsules affected by cataracts as well epithelial cells (LECs) exposed oxidative stress. Overexpression induced p53 verified a...
Oxidative stress plays an important role in the pathogenesis of cataracts. Small ubiquitin-like modifier (SUMO) proteins have great effects on cell response. Previous studies shown that TP53INP1 can arrest growth and induce apoptosis by modulating p53 transcriptional activity both are substrates SUMOylation. However, no previous research has studied effect SUMOylation oxidative response This is first study to investigate stress-induced lens epithelial injury age-related cataract formation....
To detect the expression of miR-211 in age-related cataract tissue, explore effects on lens epithelial cell proliferation and apoptosis, identify its target gene.This study used real-time quantitative polymerase chain reaction (RT-qPCR) to measure predicted gene [silent mating-type information regulation 2 homolog 1 (SIRT1)] 46 anterior capsules collected from patients. Human line (SRA01/04) cells were transfected with either mimics, mimic controls, inhibitors or inhibitor 72h after...
Thioredoxin (Trx) is an important protein that controls oxidative damage in almost all eukaryotic cells. Trx interaction (Txnip) has been reported to negatively regulate the bioavailability of and inhibit its biological function. The E3 ubiquitin ligase ITCH can specifically degrade Txnip via ubiquitination. apoptosis human lens epithelial cells (HLECs), which are highly sensitive redox caused by stress, a significant factor for development sugar cataract high‑glucose environment. However,...
To investigate the effects and mechanism of miR-211 in mediating antioxidant function lens epithelial cells affected by age-related cataracts.Real-time quantitative polymerase chain reaction (RT-qPCR) was used to detect expression anterior capsules healthy people, patients with cataracts, human cell line (SRA01/04) exposed oxidative stress. A 2', 7'-dichloro-fluorescein diacetate (DCFH-DA) probe measure levels endogenous reactive oxygen species (ROS) (hLECs) 400 µmol/L H2O2 for 1h. SRA01/04...