A5064153949- Mesenchymal stem cell research
- Systemic Lupus Erythematosus Research
- Virus-based gene therapy research
- T-cell and B-cell Immunology
- CRISPR and Genetic Engineering
- Adipose Tissue and Metabolism
- Rheumatoid Arthritis Research and Therapies
- Monoclonal and Polyclonal Antibodies Research
- Endometriosis Research and Treatment
- Pluripotent Stem Cells Research
- Reproductive System and Pregnancy
- Uterine Myomas and Treatments
- Protease and Inhibitor Mechanisms
- RNA Interference and Gene Delivery
- Pancreatic function and diabetes
- Innovation and Socioeconomic Development
- Autoimmune and Inflammatory Disorders Research
- Electrospun Nanofibers in Biomedical Applications
- Genetics and Physical Performance
- Wound Healing and Treatments
- Galectins and Cancer Biology
- Cancer-related gene regulation
- Advanced Nanomaterials in Catalysis
- Cardiovascular Disease and Adiposity
- Single-cell and spatial transcriptomics
Lomonosov Moscow State University
2015-2022
Moscow State University
2010-2017
The adult heart contains small populations of multipotent cardiac progenitor cells (CPC) that present a convenient and efficient resource for treatment myocardial infarction. Several clinical studies direct CPC delivery by injection have already been performed but showed low engraftment rate limited beneficial effects procedure. «Cell sheet» technology has developed to facilitate longer retention grafted show new directions cell-based therapy using this strategy. In study we hypothesized...
Rheumatoid arthritis (RA) is frequent systemic autoimmune disease characterized by excessive activation of collagen-specific T helper cells, and elevated level autoantibodies in the serum. Development RA associated with defect compartment regulatory CD4+Foxp3+ cells (Treg), but data concerning suppressive potential Treg population patients are contradictory depend on stage disease. In this study we aimed to characterize abundance phenotypic markers peripheral blood healthy donors compared...
Besides certain exceptions, healing of most tissues in the human body occurs via formation scar tissue, rather than restoration lost structures. After extensive acute injuries, this phenomenon substantially limits possibility function recovery and, case chronic injury, it leads to pathological remodeling organs affected. Managing outcomes damaged tissue repair is one main objectives regenerative medicine. The first priority for reaching comparative investigation mechanisms responsible...
Abstract Sympathetic neurons are important components of mesenchymal stem cells (MSCs) niche and noradrenaline regulates biological activities these cells. Here we examined the mechanisms regulation MSCs responsiveness to noradrenaline. Using flow cytometry, demonstrated that α1A adrenergic receptors isoform was most abundant in adipose tissue-derived MSCs. calcium imaging single cells, only 6.9 ± 0.8% responded by intracellular release. Noradrenaline increases sensitivity catecholamines a...
// Karina D. Rysenkova 1 , Ekaterina V. Semina 1, 2 Maxim N. Karagyaur 3 Anna A. Shmakova Daniyar T. Dyikanov Petr Vasiluev Yury P. Rubtsov 4 Kseniya Rubina and Vsevolod Tkachuk Lomonosov Moscow State University, Faculty of Medicine, Laboratory Gene Cell Technologies, 119991, Moscow, Russian Federation Federal Budgetary Organization National Cardiology Research Center Ministry Health the Federation, Institute Experimental Cardiology, 121552, Regenerative Shemyakin-Ovchinnikov Bioorganic...
Obesity is often associated with high systemic and local activity of renin-angiotensin system (RAS). Mesenchymal stem cells adipose tissue are the main source adipocytes. The aim this study was to clarify how RAS could control differentiation human derived mesenchymal (ADSCs). We examined distribution angiotensin receptor expressing in found that type 1 2 receptors co-expressed its stromal compartment, which known contain cells. To expression specifically ADSCs we have isolated them from...
Multipotent stromal cells (MSC) demonstrate remarkable functional heterogeneity; however, its molecular mechanisms remain largely obscure. In this study, we explored MSC response to hormones, which activate Gs-protein / cyclic AMP (cAMP) protein kinase A (PKA) dependent signaling, at the single cell level using genetically encoded biosensor PKA-Spark. For first time, demonstrated that about half of cultured MSCs are not able cAMP/PKA pathway, possibly due limited availability adenylyl...
Mesenchymal stem/stromal cells (MSCs) were identified in most tissues of an adult organism. MSCs mediate physiological renewal, as well regulation tissue homeostasis, reparation and regeneration. Functions are regulated by endocrine neuronal signals, noradrenaline is one the important MSC regulators. We provided flow cytometry analysis expression adrenergic receptors on surface human isolated from ten different donors. have found that profile vary significantly between also showed...
Primary adipose tissue-derived multipotent stem/stromal cells (adMSCs) demonstrate unusual signaling regulatory mechanisms, i.e., increased of sensitivity to catecholamines in response noradrenaline. This phenomenon is called “heterologous sensitization”, and was previously found only embryonic cells. Since further elucidation the molecular mechanisms that are responsible for such sensitization primary adMSCs difficult due high heterogeneity adrenergic receptor expression, we employed...
Most commonly used cell lines are readily susceptible to genome editing and present a good object for models establish disease-causing genes find ways cure diseases. However, karyotype phenotype heterogeneity between individual cells in such cultures as well multiplicity of target alleles make generation desired by single-cell cloning (used diploid cells) inapplicable. We designed tested simple approach targeted modification single sizable populations, containing multiple variants. To obtain...
Abstract Duox2 belongs to the large family of NADPH-oxidase enzymes that are implicated in immune response, vasoregulation, hormone synthesis, cell growth and differentiation via regulated synthesis H 2 O reactive oxygen species. We others have shown involved platelet-derived factor (PDGF) induced migration fibroblasts. Now, using CRISPR/Cas9-mediated genome editing we demonstrate extreme C-terminal region is required for PDGF-stimulated activity production. generated fibroblast cells stably...
Ex vivo, gene therapy is a powerful approach holding great promises for the treatment of both genetic and acquired diseases. Adeno-associated virus (AAV) vectors are safe efficient delivery system modification mesenchymal stem cells (MSC) that could maximize their therapeutic benefits. Assessment MSC viability functional activity after infection with new AAV serotypes necessary, due to tropism specific cell types. We infected human rat adipose-tissue hybrid AAV-DJ serotype carrying GFP SCF...
This article contains results of analyses angiotensin II receptors expression in human adipose tissue and stem/stromal cells isolated from tissue. We also provide here data regarding the effect on intracellular calcium mobilization derived (ADSCs). Discussion can be found (Sysoeva et al., 2017) [1].
T regulatory cells (Tregs) play a key role in the immune system due to suppression of hyperimmune response autoantigens and opportunistic enteric microorganisms. In recent years, there has been evidence that Tregs can suppress various immunoinflammatory responses wide range physiological pathological stimuli, including microorganisms, tumor cells, allogeneic grafts, fetal cells. express broad spectrum membrane molecules determine their functional activity make it possible identify these...
Objective: to investigate the count and characteristics of phenotype T regulatory cells (Treg) in peripheral blood healthy donors patients with early rheumatoid arthritis (RA), by using multicolor flow cytometry. Subjects methods . The investigation enrolled 39 RA. percentage absolute Treg (FoxP3+CD25+, surface CD152+, intracellular FoxP3+CD127, CD25+CD127, FoxP3+ICOS+, FoxP3+CD154+; FoxP3+CD274+) was determined flow-cytometry. A control group consisted 20 matched for sex age examined...
In this study, we developed a novel Cre/lox71-based system for the controlled transient expression of target genes. We used bacteriophage P1 Cre recombinase, which harbors short, highly specific DNA-binding site and does not have endogenous binding sites within mouse or human genomes. Fusing catalytically inactive form recombinase VP64 transactivation domain (VP16 tetramer), constructed artificial transcription factor Cre-VP64. This binds to lox71 promoter region gene and, therefore,...
Objective: to analyze the relationship of count FoxP3+ T regulatory cells (Tregs) clinical and laboratory parameters disease activity levels antibodies in a group patients with early rheumatoid arthritis (RA). Subjects methods. The investigation enrolled 45 RA (2010 ACR/EULAR criteria) who had not previously received treatment methotrexate, including 39 women; median age was 52.0 [32.5; 57.5] years; duration, 5 [4; 6] months, DAS28 5.01 [4.18; 5.8]; 71.1% were factor (RF) positive 88.9%...
<h3>Background</h3> Regulatory T (Treg) cells play a critical role in the regulation of peripheral immune tolerance.The ability Tregs to suppress T-cell responses,and thereby regulate reactions,points their key pathophysiology autoimmune diseases <h3>Objectives</h3> To investigate percentage and absolute number Treg blood healthy donors DMARD-naive very early rheumatoid arthritis (VERA) patients (pts) by immunocytometry analyze association between subsets RA activity <h3>Methods</h3>...