A5020682685- thermodynamics and calorimetric analyses
- Cellular transport and secretion
- Field-Flow Fractionation Techniques
- Advanced Proteomics Techniques and Applications
- Click Chemistry and Applications
- Advanced biosensing and bioanalysis techniques
- Pancreatic function and diabetes
- Monoclonal and Polyclonal Antibodies Research
- Heat shock proteins research
- Bioinformatics and Genomic Networks
- Spectroscopy Techniques in Biomedical and Chemical Research
- Autophagy in Disease and Therapy
- Plant Pathogenic Bacteria Studies
- Lipid Membrane Structure and Behavior
- Protein Structure and Dynamics
- Fungal and yeast genetics research
- Retinal Development and Disorders
- Plant Virus Research Studies
Heidelberg University
2020-2024
Heidelberg University
2014-2024
NanoTemper Technologies (Germany)
2014-2017
MicroScale Thermophoresis (MST) is a powerful technique to quantify biomolecular interactions. It based on thermophoresis, the directed movement of molecules in temperature gradient, which strongly depends variety molecular properties such as size, charge, hydration shell or conformation. Thus, this highly sensitive virtually any change properties, allowing for precise quantification events independent size nature investigated specimen. During MST experiment, gradient induced by an infrared...
Proteomics techniques can identify thousands of phosphorylation sites in a single experiment, the majority which are new and lack precise information about function or molecular mechanism. Here we present fast method to predict potential switches by mapping protein-protein interactions known structure analysing properties protein interface. We 1024 that could potentially enable disable particular interactions. tested selection these showed phosphomimetic mutations indeed affect estimate...
Munc18-1 is essential for vesicle fusion and participates in the docking of large dense-core vesicles to plasma membrane. Recent structural data suggest that conformational changes 12th helix domain 3a within Munc18-1:syntaxin complex result an additional interaction with synaptobrevin-2/VAMP2 (vesicle-associated membrane protein 2), leading SNARE formation. To test this hypothesis living cells, we examined secretion from <i>Munc18-1</i>-null mouse adrenal chromaffin cells expressing mutants...
Here, we present a simple, modular and efficient strategy that allows the 3′-terminal labeling of DNA, regardless whether it has been chemically or enzymatically synthesized isolated from natural sources. We first incorporate range modified nucleotides at 3′-terminus, using terminal deoxynucleotidyl transferase. In second step, convert incorporated nucleotides, either four highly click chemistry-type reactions, namely copper-catalyzed azide-alkyne cycloaddition, strain-promoted Staudinger...
Abstract Sec1/Munc18 proteins play a key role in initiating the assembly of N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complexes, molecular fusion machinery. Employing comparative structure modeling, site specific crosslinking by single amino acid substitutions with photoactivatable unnatural p-Benzoyl-phenylalanine (Bpa) and reconstituted vesicle docking/fusion assays, we mapped binding interface between Munc18-1 neuronal v-SNARE VAMP2 resolution. Our results show...
BackgroundPathogenic variants in STXBP1/Munc18-1 cause severe encephalopathies that are among the most common genetic neurodevelopmental disorders. Different molecular disease mechanisms have been proposed and pathogenicity prediction is limited. This study aims to define a generalized concept for STXBP1-related disorders improve prediction.MethodsA cohort of 11 disease-associated five neutral (detected healthy individuals) was tested three cell-free assays, heterologous cells primary...