A5088078573- Pregnancy and preeclampsia studies
- Nitric Oxide and Endothelin Effects
- Hormonal Regulation and Hypertension
- Birth, Development, and Health
- Renin-Angiotensin System Studies
- Estrogen and related hormone effects
- Hormonal and reproductive studies
- Receptor Mechanisms and Signaling
- Sexual Differentiation and Disorders
- Cardiovascular Issues in Pregnancy
- Neuroendocrine regulation and behavior
- Reproductive System and Pregnancy
- Reproductive Biology and Fertility
- Molecular Biology Techniques and Applications
- Neonatal Respiratory Health Research
- Eicosanoids and Hypertension Pharmacology
- Neurobiology and Insect Physiology Research
- Stress Responses and Cortisol
- Adipose Tissue and Metabolism
- Ovarian function and disorders
- Pharmacogenetics and Drug Metabolism
- Angiogenesis and VEGF in Cancer
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Connexins and lens biology
- Cancer, Hypoxia, and Metabolism
Pennsylvania State University
2020-2024
Johns Hopkins University Applied Physics Laboratory
2024
University of Wisconsin–Madison
2013-2022
Institut National de Physique Nucléaire et de Physique des Particules
2022
Meriter Hospital
2003-2021
UnityPoint Health Meriter
2019-2021
State Laboratory
2020
Orthopaedic Research Laboratories
1998-2017
University of South Florida
2017
Boston Children's Hospital
2017
The newly emerged severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) highlights the urgent need for assays that detect protective levels of neutralizing antibodies. We studied relationship among anti-spike ectodomain (anti-ECD), anti–receptor-binding domain (anti-RBD) IgG titers, and SARS-CoV-2 virus neutralization (VN) titers generated by in vitro using convalescent plasma samples from 68 patients with COVID-19. report a strong positive correlation between both anti-RBD anti-ECD...
Excessive secretion of aldosterone from the adrenal results in most common form endocrine hypertension. An understanding regulatory processes involved synthesis and release is needed to define biomolecular mechanisms controlling excessive production aldosterone. However, vitro studies regarding human have been limited because difficulties obtaining tissue subsequent isolation aldosterone-secreting glomerulosa cells. Herein we describe an adrenocortical carcinoma cell line, NCI-H295, which...
Abstract The receptor‐binding domain (RBD) of the SARS‐CoV‐2 spike protein is a candidate vaccine antigen that binds angiotensin‐converting enzyme 2 (ACE2), leading to virus entry. Here, it shown rapid conversion recombinant RBD into particulate form via admixing with liposomes containing cobalt‐porphyrin‐phospholipid (CoPoP) potently enhances functional antibody response. Antigen binding His‐tag insertion CoPoP bilayer results in serum‐stable and conformationally intact display on liposome...
Rapid uterine vasodilatation after estrogen administration is believed to be mediated by endothelial production of nitric oxide (NO) via NO synthase (eNOS). However, the mechanism(s) which activates eNOS in artery cells (UAEC) unknown. In this study, we observed that estradiol-17beta (E2) and E2-BSA rapidly (<2 min) increased total NOx UAEC vitro. This was associated with rapid phosphorylation activation but unaltered pretreatment actinomycin-D. Estrogen receptor-alpha protein detectable...
Pregnancy is characterized by elevations in uterine but not omental artery nitric oxide synthase (NOS)-specific activity. We hypothesized that increases NO production during pregnancy are associated with protein expression of the constitutive isoform, endothelial cell NOS (ecNOS), systemic arteries. Arterial NOS-specific activity and guanosine 3',5'-cyclic monophosphate (cGMP) were tested pregnant sheep presence or absence [+5 mM ethylene glycol-bis(beta-aminoethyl...
The regulation of biosynthesis the adrenal C19 steroids (the so-called androgens) remains unclear. Understanding production is important when benefits these are considered on processes and diseases associated with aging. In vitro studies defining mechanisms that regulate human have been limited because difficulties in obtaining tissue. A cell line retains differentiated functions would greatly facilitate research this area. Herein, we describe use adrenocortical tumor H295 as a model to...
Newly emerged pathogens such as SARS-CoV-2 highlight the urgent need for assays that detect levels of neutralizing antibodies may be protective. We studied relationship between anti-spike ectodomain (ECD) and anti-receptor binding domain (RBD) IgG titers, virus neutralization (VN) titers generated by two different in vitro using convalescent plasma samples obtained from 68 COVID-19 patients, including 13 who donated multiple times. Only 23% (16/68) donors had been hospitalized. also 16...
The effects of aromatase within the brain on sexual behavior have been studied in a wide variety species. Relatively few non-mating behaviors considered, despite evidence that estrogen affects many social behaviors. Testosterone promotes paternal California mouse <i>(Peromyscus californicus)</i> fathers, acting primarily via aromatization to estradiol. Virgin male mice rarely exhibit behavior, so we investigated whether changed with onset fathers. In medial preoptic area (MPOA),...
Uterine blood flow (UBF) and uterine artery endothelial nitric oxide synthase (eNOS) expression are greatest during the follicular vs. luteal phase. 17β-Estradiol (E 2 β) increases UBF elevates eNOS in ovine but not systemic arteries; progesterone (P 4 ) effects on E β changes of remain unclear. Nonpregnant ovariectomized sheep received either vehicle ( n = 10), P (0.9 g Controlled Internal Drug Release vaginal implants; 13), (5 μg/kg bolus + 6 μg · kg −1 day ; or 12). Reproductive...
During pregnancy, the uterine vasculature shows a marked increase in vasodilator production [prostacyclin (PGI2) and nitric oxide (NO)] response to number of agonists including angiotensin II (AII) ATP. As consequence vascular resistance is kept low, blood flow maximized meet needs growing fetus. Studies molecular basis underlying this change control endothelial NO PGI2 have been hampered by lack availability suitable cell model. To that end we developed characterized new ovine artery (UAEC)...
During the follicular phase of ovarian cycle, when local estrogen-to-progesterone ratio is elevated, uterine blood flow elevated. This vasodilatory response reproduced by exogenous 17β-estradiol (E 2 β) administration via a nitric oxide (NO)-mediated mechanism. We hypothesized that endogenous estrogen and E β treatment elevate expression endothelial cell-derived NO synthase (eNOS) in uterine, but not systemic, arteries. Uterine, mammary, systemic (renal and/or omental) arteries were...
Normal pregnancy and the follicular phase of ovarian cycle are both estrogen-dominated physiological states that characterized by elevations in uterine blood flow endothelial nitric oxide synthase (eNOS) protein expression artery (UA) endothelium. It is unknown if mRNA level account for changes or eNOS activity. We tested hypothesis associated with increases consequent elevated results increased circulating (NO) levels. UA were obtained from pregnant (PREG; n = 8; 110–130 days gestation;...