A5035805750- Pancreatic function and diabetes
- Calcium signaling and nucleotide metabolism
- Diabetes Management and Research
- Cell Image Analysis Techniques
- Polymer composites and self-healing
- Microfluidic and Capillary Electrophoresis Applications
- Advanced Fluorescence Microscopy Techniques
- Image Processing Techniques and Applications
- biodegradable polymer synthesis and properties
- Virtual Reality Applications and Impacts
- Muscle Physiology and Disorders
- Platelet Disorders and Treatments
- Electrospun Nanofibers in Biomedical Applications
- Hemophilia Treatment and Research
- Adipose Tissue and Metabolism
- Diverse Educational Innovations Studies
- E-Learning and Knowledge Management
- Educational Games and Gamification
- Online Learning and Analytics
- Genetics, Bioinformatics, and Biomedical Research
- Biomedical and Engineering Education
- Fibroblast Growth Factor Research
- Electrochemical sensors and biosensors
- Metabolism, Diabetes, and Cancer
- Advanced biosensing and bioanalysis techniques
University of Toronto
1998-2024
University Health Network
2021-2022
Toronto General Hospital
2021-2022
Ted Rogers Centre for Heart Research
2020
Western University
2020
Vanderbilt University
2020
St. Michael's Hospital
1998
NADPH/NADP+ redox state supports numerous reactions related to cell growth and survival; yet the full impact is difficult appreciate due organelle compartmentalization of NADPH NADP+. To study glucose-stimulated production in pancreatic beta-cell organelles, we targeted Apollo-NADP+ sensor by first selecting most pH-stable version single-color sensor. We subsequently mTurquoise2-Apollo-NADP+ various organelles confirmed activity cytoplasm, mitochondrial matrix, nucleus, peroxisome. Finally,...
Discovery is an inquiry-based STEM educational program offered free of charge by the University Toronto in partnership with
Autophagy is a physiological self-eating process that can promote cell survival or activate death in eukaryotic cells. In skeletal muscle, it important for maintaining muscle mass and function critical to sustain mobility regulate metabolism. The UV radiation resistance-associated gene (UVRAG) regulates the early stages of autophagy autophagosome maturation plays key role endosomal trafficking. This study investigated essential vivo UVRAG biology. To determine vivo, we generated...
Deep learning provides an opportunity to automatically segment and extract cellular features from high-throughput microscopy images. Many labeling strategies have been developed for this purpose, ranging the use of fluorescent markers label-free approaches. However, differences in channels available each respective training dataset make it difficult directly compare effectiveness these across studies. Here, we explore models using subimage stacks composed sampled larger, “hyper-labeled,”...
We studied the effects of porcine factor VIII (P-FVIII; Hyate:C) and other coagulation products employed in management patients with hemophilia A, on platelet activation vitro. Exposure normal resting platelets to P-FVIII resulted activation, as manifested by increased expression surface markers CD62, CD63, activated-GPIIbIIIa, activation-induced modulation membrane glycoproteins CD41, CD42, CD36. In contrast, was not observed after exposure human FVIII, FEIBA, recombinant FVIIa, or...
Abstract The generation of pancreatic endocrine progenitor cells is an important step in the differentiation beta from human pluripotent stem (hPSC). This stage marked by expression Nkx6.1, a transcription factor with well understood downstream targets but unclear upstream regulators. In hPSC differentiation, Nkx6.1 strongly induced nicotinamide, derivative vitamin B3, which has three known functions within cell. Nicotinamide inhibits two classes enzymes as poly-ADP-ribose polymerases...
Abstract Deep learning provides an opportunity to automatically segment and extract cellular features from high-throughput microscopy images. Many labeling strategies have been developed for this purpose, ranging the use of fluorescent markers label-free approaches. However, differences in channels available each respective training dataset make it difficult directly compare effectiveness these across studies. Here we explore models using subimage stacks composed sampled larger,...
Abstract The shift to distance learning in response the COVID-19 pandemic has presented teachers and students with several challenges. Teachers have found themselves quickly creating materials provide equal or greater educational opportunity engagement as in-person instruction. This is met parallel increased demand on independently manage their coursework absence of supervision, support, peer interaction. In this work, we describe our approach observations transitioning Discovery, a...
High school science, technology, engineering, and math (STEM) curricula are generally knowledge-based in methodology focus on content delivery preparation for post-secondary study. However, the rapid technological change at cutting edge rate of global integration STEM highlight importance developing a holistic critical thinking framework student learning. In 2016, graduate students Institute Biomaterials & Biomedical Engineering created Discovery, collaborative high educational program...
ABSTRACT NADPH/NADP + redox state supports numerous reactions related to cell growth and survival; yet the full impact is difficult appreciate due organelle compartmentalization of NADPH NADP . To study glucose-stimulated production in pancreatic beta-cell organelles, we targeted Apollo-NADP sensor by first selecting most pH stable version single colour sensor. We subsequently mTurquoise2-Apollo-NADP various organelles confirmed activity cytoplasm, mitochondrial matrix, nucleus, peroxisome....
Abstract Introduction: Curriculum for high school students who participate in a science, technology, engineering, and math (STEM) are generally focused on delivery of knowledge effort to ensure achievement learning outcomes related important scientific concepts preparation post-secondary study. Considering global environment rapid technological change at the cutting edge, curriculum STEM quickly loses its relevance application, highlighting importance focusing development critical thinking...