A5039255995- 3D Printing in Biomedical Research
- Tissue Engineering and Regenerative Medicine
- Micro and Nano Robotics
- Electrospun Nanofibers in Biomedical Applications
- Spectroscopy Techniques in Biomedical and Chemical Research
- Neuroscience and Neural Engineering
- Modular Robots and Swarm Intelligence
- Microfluidic and Bio-sensing Technologies
- Gold and Silver Nanoparticles Synthesis and Applications
- Advanced Sensor and Energy Harvesting Materials
- Ion channel regulation and function
- Connexins and lens biology
- Advanced biosensing and bioanalysis techniques
- Cardiac electrophysiology and arrhythmias
- Photoreceptor and optogenetics research
- Cellular Mechanics and Interactions
- Advanced Materials and Mechanics
- Innovative Microfluidic and Catalytic Techniques Innovation
- Advanced Memory and Neural Computing
- Nicotinic Acetylcholine Receptors Study
- Congenital heart defects research
- Ginseng Biological Effects and Applications
- Single-cell and spatial transcriptomics
- Analytical Chemistry and Sensors
- Metal and Thin Film Mechanics
Harvard University
2021-2025
Johns Hopkins University
2014-2023
Johns Hopkins Medicine
2020-2023
Wenzhou Medical University
2022
Beth Israel Deaconess Medical Center
2022
Tsinghua University
2012-2015
State Key Laboratory of Chemical Engineering
2015
Helical alignments within the heart's musculature have been speculated to be important in achieving physiological pumping efficiencies. Testing this possibility is difficult, however, because it challenging reproduce fine spatial features and complex structures of using current techniques. Here we report focused rotary jet spinning (FRJS), an additive manufacturing approach that enables rapid fabrication micro/nanofiber scaffolds with programmable three-dimensional geometries. Seeding these...
Given the heterogeneous nature of cultures, tumors, and tissues, ability to capture, contain, analyze single cells is important for genomics, proteomics, diagnostics, therapeutics, surgery. Moreover, surgical applications in small conduits body such as cardiovascular system, there a need tiny tools that approach size red blood traverse vessels capillaries. We describe fabrication arrayed or untethered cell grippers composed biocompatible bioresorbable silicon monoxide dioxide. The energy...
Hookworm inspired, autonomous latching on the gastrointestinal mucosa extends in vivo retention of a drug delivery device.
Single cell manipulation is important in biosensing, biorobotics, and quantitative analysis. Although microbeads, droplets, microrobots have been developed previously, it still challenging to simultaneously excise, capture, manipulate single cells a biocompatible manner. Here, we describe untethered grippers, that can be remotely guided actuated on-demand actively capture or excise individual few cells. We novel molding method micropattern thermally responsive wax layer for motion actuation....
Abstract Reported is a new shell‐based spectroscopic platform, named mechanical trap surface‐enhanced Raman spectroscopy (MTSERS), for simultaneous capture, profiling, and 3D microscopic mapping of the intrinsic molecular signatures on membrane single live cells. By leveraging functionalization inner surfaces MTs with plasmonic gold nanostars, conformal contact cell membrane, MTSERS permits excellent signal enhancement, reliably detects signatures, allows non‐perturbative, multiplex surface...
In order to incorporate the extraordinary intrinsic thermal, electrical, mechanical, and optical properties of graphene with three dimensional (3D) flexible substrates, we introduce a solvent-driven self-folding approach using graphene-polymer bilayers. A polymer (SU-8) film was spin coated atop chemically vapor deposited films on wafer substrates bilayers were patterned or without metal electrodes photolithography, thin deposition, etching. After patterning, released from they self-folded...
Abstract Microelectrode arrays (MEAs) have proved to be useful tools for characterizing electrically active cells such as cardiomyocytes and neurons. While there exist a number of integrated electronic chips recording from small populations or even single cells, they rely primarily on the interface between 2D flat electrodes. Here, an approach that utilizes residual stress‐based self‐folding create individually addressable multielectrode interfaces wrap around cell in 3D function electrical...
Biofabricated mass-producible arteries containing layered and aligned cells matrix exhibit enhanced cellular function.
In biomimetic design, researchers recreate existing biological structures to form functional devices. For biohybrid robotic swimmers assembled with tissue engineering, this is problematic because most devices operate at different length scales than their naturally occurring counterparts, resulting in reduced performance. To overcome these challenges, here, we demonstrate how machine learning–directed optimization (ML-DO) can be used inform the design of a robot, outperforming other nonlinear...
Abstract Reported is a new shell‐based spectroscopic platform, named mechanical trap surface‐enhanced Raman spectroscopy (MTSERS), for simultaneous capture, profiling, and 3D microscopic mapping of the intrinsic molecular signatures on membrane single live cells. By leveraging functionalization inner surfaces MTs with plasmonic gold nanostars, conformal contact cell membrane, MTSERS permits excellent signal enhancement, reliably detects signatures, allows non‐perturbative, multiplex surface...
The development of magnetically powered microswimmers that mimic the swimming mechanisms microorganisms is important for lab‐on‐a‐chip devices, robotics, and next‐generation minimally invasive surgical interventions. Governed by their design, most previously described untethered swimmers can be maneuvered only varying direction applied rotational magnetic fields. This constraint makes even state‐of‐the‐art incapable reversing motion without a prior change in field rotation, which limits...
Light is recognized as an accurate and noninvasive tool for stimulating excitable cells. Here, we report on a non-genetic approach based organic molecular phototransducers that allows wiring- electrode-free tissue modulation. As proof of concept, show photostimulation in vitro cardiac microphysiological model mediated by amphiphilic azobenzene compound preferentially dwells the cell membrane. Exploiting this optical stimulation technology could be disruptive highly resolved stimulation.
In tissues and organs, the extracellular matrix (ECM) helps maintain inter- intracellular architectures that sustain structure–function relationships defining physiological homeostasis. Combining fiber scaffolds cells to form engineered is a means of replicating these relationships. Engineered tissues' are designed mimic topology chemical composition ECM network. Here, we asked how found in heart compare their propensity align cytoskeleton self-organize response topological cues fibrous...
While membrane proteins such as ion channels continuously turn over and require replacement, the mechanisms of specificity efficient channel delivery to appropriate subdomains remain poorly understood. GJA1-20k is a truncated Connexin43 (Cx43) isoform arising from translation initiating at an internal start codon within same parent GJA1 mRNA requisite for full-length Cx43 trafficking cell borders. does not have full transmembrane domain, it known how enables forward hemichannels. Here, we...
Studying the behavior of electroactive cells, such as firing dynamics and chemical secretion, is crucial for developing human disease models therapeutics. Following recent advances in cell culture technology, traditional monolayers are optimized to resemble more 3D, organ‐like structures. The biological electrochemical complexity these structures requires devices with adaptive shapes novel features, precise electrophysiological mapping stimulation case brain‐ heart‐derived tissues. However,...
In recent years, untethered microdevices have drawn significant attention due to their small size, weight and ability exert forces without the need for wires or tethers. Such are relevant implantable biomedical devices, miniature robotics, minimally invasive surgery, microelectromechanical systems. While devices using these actuators been widely utilized in pick-and-place biopsy applications, exerted by yet be characterized analyzed. Lack of precise force measurements validated models...
Abstract Previously, we identified that GJA1-20k, an internally translated isoform of Connexin 43, mediates actin-dependent protective form mitochondrial fission (Shimura, Nuebel et al. 2021). We found when GJA1-20k is present, bands actin surround mitochondria at locations enriched with inducing which generates less oxygen free radicals, protecting hearts subjected to ischemia-reperfusion injury. Here, report a direct binding protein and thereby identify the mechanism by able recruit...