Existing local drug delivery systems for periodontitis suffer from poor antibacterial effect and unsatisfied periodontal regeneration. In this study, a smart gingipain-responsive hydrogel (PEGPD@SDF-1) was synthesized as an environmentally sensitive carrier on-demand delivery. The PEGPD@SDF-1 polyethylene glycol diacrylate (PEG-DA) based scaffolds, dithiothreitol (DTT), novel designed functional peptide module (FPM) via Michael-type addition reaction, the further loaded with stromal cell...

Abstract Interleukin (IL)-6 is known to indirectly enhance osteoclast formation by promoting receptor activator of nuclear factor kappa-B ligand (RANKL) production osteoblastic/stromal cells. However, little about the direct effect IL-6 on osteoclastogenesis. Here, we determined effects and its soluble (sIL-6R) RANKL-induced precursors in vitro . We found IL-6/sIL-6R significantly promoted suppressed differentiation induced low- (10 ng/ml) high-level (50 RANKL, respectively. Using a bone...

Bone morphology protein-2 (BMP-2) encapsulated chitosan/chondrotin sulfate nanoparticles (CHI/CS NPs) are developed to enhance ectopic bone formation on biphasic calcium phosphate (BCP) scaffolds. BMP-2 contained CHI/CS NPs were prepared by a simple and mild polyelectrolyte complexation process. It does not involve harsh organic solvents high temperature, therefore retain growth factors activity. These immobilized BCP scaffolds, realize the sustained release of from The bare NP loaded...

Abstract Background The incidence of diabetic osteoporosis is increasing. This article evaluates the effect combination treatment with hypoglycemic drug exendin-4 (Ex-4) and vitamin D analog eldecalcitol (ED-71) on improving explores relevant mechanism action. Method Micro-CT, HE staining, immunohistochemistry, qPCR ELISA were used to evaluate impact Ex-4 ED-71 bone formation macrophage polarization in a mouse model vivo. Immunofluorescence, flow cytometry characterize type macrophages...

Abstract Current treatment of postmenopausal osteoporosis (PMOP) focuses on systemic administration medication, neglecting to proactively modulate the local microenvironment skeletal system for superior therapeutic performance. Eldecalcitol (ED‐71), a novel drug treating osteoporosis, still requires research overcome high‐frequency delivery and low‐utilization. Here, bone‐targeting multishelled nanoparticles are developed step‐wise release ED‐71. The achieved by using chitosan/alginate outer...

Biodegradable zinc (Zn) alloys are promising implant materials to restore bone defects in orthopedics. Adding silver (Ag) Zn not only enhances its mechanical properties but also improves antibacterial properties. However, the regular pattern of Ag composition on Zn-xAg remains unclear. In this paper, Zn-Ag with different contents ranging from 0.5-6.0% were prepared by casting process and systematically investigated both vitro vivo. Mechanical tests suggest that increase content enhanced...

Estrogen deficiency is the main reason of postmenopausal osteoporosis. Eldecalcitol (ED-71) a new active vitamin D analogue clinically used in treatment We aimed to investigate whether EphrinB2-EphB4 and RANKL/RANK/OPG signaling cooperate mediating process osteoporosis by ED-71.

Abstract Background Porphyromonas gingivalis lipopolysaccharide (LPS) plays a major role in the initiation and progression of chronic periodontitis. Human gingival fibroblasts (HGFs) interact with bacteria or bacterial products trigger inflammatory signaling pathways that destroy periodontal tissues. RhoA regulates cytokine production various cell types. This study investigated Rho‐kinase inhibitor Y‐27632 LPS‐induced nuclear factor‐kappa B (NF‐κB) p‐38 mitogen‐activated protein kinase...

Background: Diabetes-associated periodontitis (DPD) is an inflammatory and destructive disease of periodontal tissues in the diabetic population. The manifested as more severe destruction difficult to treat when compared with (PD). Eldecalcitol (ELD) a novel active vitamin D3 analog; however, little clinical evidence available on its role improving PD DPD, specific mechanisms remain unclear. In this study, we evaluated preventative effects ELD toward DPD explored underlying molecular...

Abstract Background and Objective Prevention of periodontal bone resorption triggered by Porphyromonas gingivalis ( P. ) is crucial for dental stability. Capsaicin, known as the pungent ingredient chili peppers, can activate key signaling molecules involved in osteogenic process. However, effect capsaicin on osteogenesis ligament stem cells (PDLSCs) under inflammation remains elusive. Methods culture suspension was added to mimic inflammatory status after pretreatment. The effects PDLSCs,...

This study presents an improved weed detection model, ADL-YOLOv8, designed to enhance accuracy for small targets while achieving model lightweighting. It addresses the challenge of attaining both high and low memory usage in current intelligent weeding equipment. By overcoming this issue, research not only reduces hardware costs automated impurity removal equipment but also enhances software recognition accuracy, contributing reduced pesticide use promotion sustainable agriculture. The...

Abstract Periodontitis is an inflammatory disease leading to tooth loss, alveolar bone absorption and disorder of masticatory function. Guided tissue regeneration (GTR) one the most common strategies for lost periodontium. During surgical process, barrier membranes, osteoinductive/osteoconductive materials should be placed, respectively, which may increase risks infection, bleeding, difficulty operation. Here, we introduced a new kind hydroxyapatite (HAp) nanowires modified polylactic acid...

Diabetes exacerbates the occurrence and severity of periodontitis, pathogenesis diabetic periodontitis (DPD) is influenced by delayed resolution inflammation. Eldecalcitol (ED-71) has shown promise in preventing bone loss DPD. We herein aimed to investigate role ED-71 inflammatory regression phase DPD elucidate underlying mechanisms. Type-2 diabetes was induced streptozotocin injection Wistar rats, explore vivo effect on macrophage efferocytosis, ligation combined with lipopolysaccharide....

During the rice harvesting process, severe occlusion and adhesion exist among multiple targets, such as rice, straw, leaves, making it difficult to accurately distinguish between grains impurities. To address current challenges, a lightweight semantic segmentation algorithm for impurities based on an improved SegFormer network is proposed. make full use of extracted features, decoder was redesigned. First, Feature Pyramid Network (FPN) introduced optimize structure, selectively fusing...

<title>Abstract</title> Objective Diabetes induces disorders in macrophage immunometabolism, leading to increased destruction of periodontal tissue. Identifying key factors restore metabolic alterations and promote resolution inflammation remains an unmet objective. Methods In the present study, effect efferocytosis on inflammatory regression tissue repair was assessed using diabetic disease (DPD) model. The mitochondrial function macrophages cultured under different conditions <italic>in...

Recognizing traffic signs is crucial for autonomous driving systems, as it significantly impacts their safety and dependability. However, challenges like the diminutive size of objects intricate background environments limit effectiveness current object detection models. To improve small sign detection, this research introduces an enhanced algorithm built on YOLOv10. First, a custom-designed layer detecting integrated into neck section network, enhancing feature extraction process these...

Abstract Background One strategy to delay bone loss in periodontitis involves maintaining the osteogenic differentiation function of periodontal ligament stem cells (PDLSCs). The core circadian gene BMAL1 influences fate mesenchymal and is essential for regulating pyroptosis, apoptosis, necroptosis. PANoptosis, a novel form programmed cell death, simultaneously activates all 3 pathways. This study focuses on role BMAL1, process impairment PDLSCs. Methods A mouse model was established...

Abstract To improve the accuracy of load predictions in pressure regulation stations, this research presents a novel deep learning approach for signal analysis, based on U-Net-Bidirectional Long Short-Term Memory-Attention architecture. The framework starts with an extensive feature extraction from input data using its convolutional component. Following this, it identifies dependencies among elements by employing self-attention mechanism. processed is then fed into Bidirectional Memory layer...