The aims of this study were to identify hydrogen sulfide (H2S)-producing bacteria among tongue biofilm microflora and investigate the relationship between bacterial flora H2S levels in mouth air. Oral malodour 10 subjects (age 21–56 years) assessed by gas chromatography, Breathtron organoleptic scores. Based on these assessments, divided into two groups: an odour group a no/low group. Tongue coatings sampled spread onto Fastidious Anaerobe Agar plates containing 0.05 % cysteine, 0.12...

Dental caries is initiated by demineralization of the tooth surface through acid production sugar metabolism supragingival plaque microflora. To elucidate metabolic system, we used CE-MS to perform metabolomics central carbon metabolism, EMP pathway, pentose-phosphate and TCA cycle in supra- gingival representative oral bacteria, Streptococcus Actinomyces. Supragingival contained all targeted metabolites except erythrose 4-phosphate pathway. After glucose rinse, 6-phosphate, fructose...

Dental caries is initiated by demineralization of the tooth surface through acid production from sugar plaque biofilm. Fluoride and xylitol have been used worldwide as caries-preventive reagents, based on in vitro-proven inhibitory mechanisms bacterial production. We attempted to confirm fluoride vivo performing metabolome analysis central carbon metabolism supragingival using combination capillary electrophoresis a time-of-flight mass spectrometer. (225 900 ppm F − ) inhibited lactate 10%...

The prevalence of dental caries is still high around the world. Dental initiated when teeth are exposed to acid, such as lactic produced via carbohydrate metabolism by acidogenic microorganisms. Veillonella species, which among major oral microorganisms, considered be beneficial bacteria due their ability convert acid weaker acids and produce NO 2 – from 3 , thought good for both general health. Therefore, it clear that there a need elucidate biochemical characteristics production in...

Indigenous oral bacteria in the tongue coating such as Veillonella have been identified main producers of hydrogen sulfide (H2S), one major components malodor. However, there is little information on physiological properties H2S production by metabolic activity and environmental factors which may affect production. Thus, present study, H2S-producing growing cells, resting cell extracts species effects factors, including pH lactate, were investigated. Type strains atypica, dispar, parvula...

Abstract Acetaldehyde is known to be carcinogenic and produced by oral bacteria. Thus, bacterial acetaldehyde production might contribute cancer. Therefore, we examined from ethanol glucose under various conditions mimicking the cavity clarified metabolic pathways responsible for production. Streptococcus mitis , S . salivarius mutans Neisseria mucosa N sicca were used. The metabolism was conducted at pH 5.0–8.0 aerobic anaerobic conditions. of organic acids measured with gas chromatography...

Bifidobacterium is frequently detected in early childhood caries and white spot lesions, indicating that it a novel caries-associated bacterium. known to possess unique metabolic pathway, the "bifid shunt", which might give cariogenic potential by increasing its acid production. Thus, we evaluated acid-producing activity of sensitivity fluoride, preventive reagent. B. longum, dentium, Streptococcus mutans were used. Acid-producing was measured using pH-stat absence presence fluoride under...

Scardovia wiggsiae has been detected from caries in children and adolescents suggested to be a caries-associated microorganism. To investigate the cariogenic potential of S. wiggsiae, we examined carbohydrate metabolism acid productivity, fluoride sensitivity mechanism by which inhibits metabolism, this bacterium. metabolized glucose reduced environmental pH 3.5. It mainly produced acetic glucose, together with small amounts lactic formic acid. The 50% inhibitory concentration for production...

Abstract Recently, it was suggested that the nitrite (NO 2 − ) produced from NO 3 by oral bacteria might contribute to and general health. Therefore, we aimed clarify detailed information about bacterial -production in biofilm. Dental plaque tongue-coating samples were collected, then -producing activity measured. Furthermore, composition of population identified using Griess reagent-containing agar overlay method molecular biological method. per mg wet weight varied among individuals but...

It has been suggested that green tea-derived epigallocatechin gallate (EGCG), which antimicrobial properties, might help prevent dental caries. However, the detailed properties of EGCG remain unclear. In this study, were evaluated by examining its bactericidal activity, inhibitory effects against bacterial growth, acid production, acidic end-product formation, and sugar uptake (phosphoenolpyruvate-dependent phosphotransferase system, PEP-PTS activity), on aggregation, using monocultured...

Increasing evidence suggests that aerobic glycolysis is related to the progression of oral squamous cell carcinoma (OSCC). Hence, we focused on glycolysis-related gene sets screen for potential therapeutic targets OSCC. The expression profiles OSCC samples and normal controls were obtained from Cancer Genome Atlas (TCGA). Then, differentially expressed selected official GSEA website following extraction core genes (DECGs). Subsequently, tried build a risk model basis DECGs predict prognosis...

Epigallocatechin-3-gallate (EGCG), a catechin abundant in green tea, exhibits antibacterial activity. In this study, the antimicrobial effects of EGCG on periodontal disease-associated bacteria (Porphyromonas gingivalis, Prevotella intermedia, nigrescens, Fusobacterium nucleatum, and periodontium) were evaluated compared with its Streptococcus mutans, caries-associated bacterium.

To determine the characteristics of endodontic microbiome.

ABSTRACT Actinomyces naeslundii and Schaalia odontolytica belong to the most predominant nitrite‐producing bacteria in oral microbiome. Nitrite has antibacterial vasodilatory effects that may contribute maintaining systemic health. We have previously elucidated metabolic characteristics of activity Veillonella species environmental factors. However, this is still unknown for species. Furthermore, these are thought degrade nitrite. Therefore, study aimed comprehensively elucidate factors (pH,...

Extracellular DNA (eDNA) is one of the core components extracellular matrix (ECM) in biofilms and provides attachment sites for microbes other ECM components. However, little known about functions underlying mechanisms eDNA cariogenicity dental plaque biofilms. A recent study demonstrated that conformational diversity exists biofilms, transition from right-handed (B-DNA) to left-handed (Z-DNA) associated with structural stability pathogenicity Caries-related biofilm a complex multispecies...

ABSTRACT Background The oral microbiome has been shown to be associated with the development of squamous cell carcinoma (OSCC). Research primarily focused on elucidating oncogenic mechanisms specific pathogens by comparing microbiomes OSCC and normal tissues. However, characteristics in precancerous state remain less understood, as does influence metabolic environmental factors OSCC‐associated microbiomes. Methods In this study, we analyzed mucosa‐associated normal, precancerous, lesions...

Introduction: Dental plaque pH decreases to about 4 through bacterial fermentation of carbohydrates and this low is maintained for from several minutes an hour. Repeated acidification causes demineralization the tooth surface, resulting in caries formation. The also influences bacteria. Severe kills bacteria efficiently, while physiological acidification, condition occurring plaque, partially may impair growth ability. We, therefore, investigated effects on representative caries‐related...

Ketone bodies including acetone are disease biomarkers for diabetes that sometimes causes severe ketoacidosis. The present study was undertaken to clarify the significance of exhaled and plasma ketone at bedside in a clinical setting. oral glucose tolerance test (OGTT) performed 10 healthy Japanese volunteers (five females five males). Exhaled breath volatile sulfide compounds (VSCs) mouth air were measured simultaneously with blood sampling during OGTT using portable gas chromatograph...

<b><i>Introduction:</i></b> The purpose of this study was to investigate the effects nitrate and nitrite on pH-lowering activity human plaque, nitrite-producing -degrading activities their correlation. <b><i>Methods:</i></b> Nitrate were added plaque suspensions collected from buccal aspect maxillary molars patients visiting a general dental clinic, changes in pH measured with without glucose addition. Nitrite-producing evaluated by adding...

Although Candida species are thought to contribute dental caries, their acid production under anaerobic conditions and susceptibility fluoride have not been thoroughly studied. We therefore investigated the growth, production, effect of on species. Aerobic from glucose its end-products aerobic conditions, enolase activity were measured in C. albicans non-Candida-albicans-Candida (NCAC) (C. tropicalis, parapsilosis, maltosa, glabrata), these abilities was evaluated. All produced acids acetate...