A5037932211- CRISPR and Genetic Engineering
- RNA modifications and cancer
- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- RNA Research and Splicing
- Epigenetics and DNA Methylation
- Genomics and Chromatin Dynamics
- Cancer-related gene regulation
- Bacteriophages and microbial interactions
- Genomics and Phylogenetic Studies
- Chromosomal and Genetic Variations
- Bacterial Genetics and Biotechnology
- Genetics, Bioinformatics, and Biomedical Research
- Genetics and Neurodevelopmental Disorders
- DNA and Nucleic Acid Chemistry
- RNA and protein synthesis mechanisms
- DNA Repair Mechanisms
- Bioinformatics and Genomic Networks
- Biomedical Text Mining and Ontologies
International Institute of Molecular and Cell Biology
2016-2023
Institute of Biochemistry and Biophysics, Polish Academy of Sciences
2017-2021
Universitat Politècnica de València
2010-2012
RNA interference is a powerful experimental tool for knockdown, but not all organisms are amenable. Here, we provide proof of principle demonstration that type III Csm effector complex can be used programmable mRNA transcript degradation in eukaryotes. In zebrafish, Streptococcus thermophilus (StCsm) proved effective knockdown maternally expressed EGFP germ cells Tg(ddx4:ddx4-EGFP) fish. It also led to significant, albeit less drastic, fluorescence reduction at one day postfertilization...
Abstract Histone lysine-specific methyltransferase 2 (KMT2A-D) proteins, alternatively called mixed lineage leukemia (MLL1-4) mediate positive transcriptional memory. Acting as the catalytic subunits of human COMPASS-like complexes, KMT2A-D methylate H3K4 at promoters and enhancers. contain understudied highly conserved triplets a quartet plant homeodomains (PHDs). Here, we show that all clustered (multiple) PHDs localize to well-defined loci H3K4me3 H3 acetylation-rich active Surprisingly,...
Abstract Current methods of CRISPR-Cas9-mediated site-specific mutagenesis create deletions and small insertions at the target site which are repaired by imprecise non-homologous end-joining. Targeting Cas9 nuclease relies on a short guide RNA (gRNA) corresponding to genome sequence approximately intended intervention. We here propose an improved version CRISPR-Cas9 editing that two complementary RNAs instead one. Two delimit intervention allow precise deletion several nucleotides site. As...
Many modification-dependent restriction endonucleases (MDREs) are fusions of a PUA superfamily modification sensor domain and nuclease catalytic domain. EVE domains belong to the superfamily, present in MDREs combination with HNH domains. Here, we biochemical characterization EVE-HNH endonuclease VcaM4I crystal structures protein alone, bound either 5mC modified dsDNA or 5mC/5hmC containing ssDNA. The is moderately specific for DNA according EMSA experiments. It flips nucleotide, accommodate...
The type II and V CRISPR effector nucleases Cas9 Cpf1 are âuniversal” DNA endonucleases, which can be programmed by an appropriate crRNA or sgRNA strand to cleave almost any duplex at a preselected position (constrained only short, so-called PAMs). In this review, we briefly introduce bacterial adaptive immunity as the biological context in proteins operate, then present structural insights that have been obtained last two three years illustrate mode of operation these proteins. We...
Restriction endonucleases naturally target DNA duplexes. Systematic screening has identified a small minority of these enzymes that can also cleave RNA/DNA heteroduplexes and may therefore be useful as tools for RNA biochemistry. We have chosen AvaII (G↓GWCC, where W stands A or T) representative this group restriction detailed characterization. Here, we report crystal structures alone, in specific complex with partially cleaved dsDNA, scanning an hybrid. The reveals novel form semi-specific...
ABSTRACT RNA interference (RNAi) is a powerful experimental tool for knockdown, but not all organisms are amenable. Here, we provide “proof of principle” demonstration that CRISPR endoribonucleases can be used programmable mRNA transcript degradation. Using zebrafish as the animal model and Csm(crRNA) complexes endoribonucleases, have targeted transgenic EGFP expressed from variety promoters. A drastic decrease fluorescence was achieved in germ cells vasa:EGFP line. Weaker effects were also...
ABSTRACT Histone lysine-specfic methyltransferase 2 (KMT2A-D) proteins, alternatively called mixed lineage leukaemia (MLL1-4) mediate positive transcriptional memory. As the catalytic subunits of human COMPASS-like complexes, they methylate H3K4 at promoters and enhancers. KMT2A-D contain understudied highly conserved triplets a quartet plant homeodomains (PHDs). Here, we show that all clustered PHDs localise to well-defined loci H3K4me3 H3 acetylation-rich active Surprisingly, observe...