A5005400992- Antioxidant Activity and Oxidative Stress
- Free Radicals and Antioxidants
- Genomics, phytochemicals, and oxidative stress
- Glutathione Transferases and Polymorphisms
- Plant tissue culture and regeneration
- Bone Tissue Engineering Materials
- Vitamin C and Antioxidants Research
- Bioactive Compounds and Antitumor Agents
- Viral Infectious Diseases and Gene Expression in Insects
- Retinoids in leukemia and cellular processes
- Plant Parasitism and Resistance
- Bone Metabolism and Diseases
- Cancer, Hypoxia, and Metabolism
- Dental Implant Techniques and Outcomes
- Analytical Methods in Pharmaceuticals
- Analytical Chemistry and Sensors
- Molecular Biology Techniques and Applications
- Cancer, Lipids, and Metabolism
- Eicosanoids and Hypertension Pharmacology
- Advanced Proteomics Techniques and Applications
- Toxin Mechanisms and Immunotoxins
- Estrogen and related hormone effects
- Cancer Research and Treatment
- Healthcare and Environmental Waste Management
- Phagocytosis and Immune Regulation
Rudjer Boskovic Institute
2011-2024
Oxidative stress often affects the structure and metabolism of lipids, which in case polyunsaturated free fatty acids (PUFAs) leads to a self-catalysed chain reaction lipid peroxidation (LPO). The LPO PUFAs formation various aldehydes, such as malondialdehyde, 4-hydroxynonenal (4-HNE), 4-hydroxyhexenal, 4-oxo-2-nonenal. Among reactive 4-HNE is major bioactive product LPO, has high affinity for binding proteins. This review briefly discusses available information on applicability assessment...
This study used malondialdehyde (MDA) determination by HPLC and enzymatic assays for total serum peroxides antioxidant capacity to evaluate oxidative stress in 47 healthy full-term small-for-gestational age (SGA) newborns vs 67 appropriate-for-gestational (AGA) newborns. Blood samples were collected at delivery from umbilical cord artery vein peripheral blood of the babies on third day after birth. mothers also compared with 29 normal non-pregnant women (NPW). Serum peroxide values...
Oxidative stress and lipid peroxidation play important roles in numerous physiological pathological processes, while the bioactive products of peroxidation, hydroperoxides reactive aldehydes, act as mediators redox signaling normal malignant cells. Many types cancer, including osteosarcoma, express altered pathways. Such pathways protect cancer cells from cytotoxic effects oxidative stress, thus supporting transformation, eventually anticancer therapies associated with stress. In this...
Abstract The product of lipid peroxidation, 4‐hydroxy‐2‐nonenal (HNE) is known to cause cell death at high concentrations, while lower concentrations it can influence proliferation and differentiation. In our experiments we used human osteosarcoma cells (HOS), test the HNE on proliferation, differentiation induction apoptosis. Apoptosis was estimated by TiterTACS™ TUNEL test. were in parallel counted DAPI staining method distinguish between apoptotic necrotic as well define proportion...
Abstract It is assumed that oxidative damage caused by reactive oxygen species (ROS) from activated neutrophil granulocytes may contribute to pathology of tumors. ROS are crucial in neutrophil‐mediated tumor cell lysis. The present study focused on the burst and antitumorous activities neutrophils when challenged with Walker carcinoma W256. Survival growth dynamics were monitored vivo , while proliferation mixed was studied vitro together generation/release respiratory products, primarily 1...
Although Aloe vera contains numerous bioactive components, the activity principles of widely used A. extracts are uncertain. Therefore, we analyzed effects genuine aqueous extract (AV) on human cells with respect to hydrogen peroxide (H₂O₂) and 4-hydroxynonenal (HNE). Fully developed leaves were harvested for vitamin C, carotenoids, total soluble phenolic content, antioxidant capacity. Furthermore, cervical cancer (HeLa), microvascular endothelial (HMEC), keratinocytes (HaCat), osteosarcoma...
Tumor growth is associated with oxidative stress, which causes lipid peroxidation. The most intensively studied product of peroxidation 4-hydroxy-2-nonenal (HNE), considered as a "second messenger free radicals" that binds to proteins and acts growth-regulating signaling factor. incidence squamous cell carcinoma the oropharynx smoking, alcohol infection human papilloma virus (HPV), increasing world-wide. aim this retrospective study involving 102 patients was determine immunohistochemical...
4-Hydroxynonenal (HNE) is a major aldehydic product of lipid peroxidation known to exert several biological effects. Normal and malignant cells the same origin express different sensitivity HNE. We used human osteosarcoma (HOS) in stages differentiation vitro, showing differences mitosis, DNA synthesis, alkaline phosphatase (ALP) staining. Differentiated HOS showed decreased proliferation (3H-thymidine incorporation), viability (thiazolyl blue tetrazolium bromide-MTT), increased apoptosis...
Although the COVID-19 pandemic has ended, it is important to understand pathology of severe SARS-CoV-2 infection associated with respiratory failure and high mortality. The plasma proteome, including protein modification by lipid peroxidation products in survivors (COVID-19; n = 10) deceased individuals (CovDeath; was compared samples collected upon admission hospital, when there no difference their status, that healthy (Ctr; 10). obtained results show development strongly alters expression...
Tissue regeneration is a complex biological process of vital importance since it allows renewal damaged cells and organs. The healing long bones large joints often extended or incomplete primarily in elderly people polytraumatized patients. Various attempts are made to solve this severe medical social problem by developing novel bioactive materials, among which glass the most attractive because its osteoconductive osteostimulative properties. Lipid peroxidation defined as an important...