A5021667016- Plant biochemistry and biosynthesis
- Enzyme Structure and Function
- Protein Structure and Dynamics
- Microbial Metabolic Engineering and Bioproduction
- Photosynthetic Processes and Mechanisms
- Biochemical Acid Research Studies
- Enzyme Catalysis and Immobilization
- Antioxidant Activity and Oxidative Stress
- RNA and protein synthesis mechanisms
- ATP Synthase and ATPases Research
- Bacterial Genetics and Biotechnology
- Lipid metabolism and biosynthesis
University of California, Los Angeles
2014-2022
Great Lakes Bioenergy Research Center
2014
Significance Microbially produced aliphatic alcohols are important biocommodities but exert toxic effects on cells. Understanding the mechanisms by which these inhibit microbial growth and generate resistant microbes will provide insight into physiology improve prospects for biotechnology biofuel production. We find that Escherichia coli ribosomes RNA polymerase mechanistically affected ethanol, identifying ribosome decoding center as a likely target of ethanol-mediated conformational...
The high yields required for the economical production of chemicals and fuels using microbes can be difficult to achieve due complexities cellular metabolism. An alternative performing biochemical transformations in is build pathways vitro, an approach we call synthetic biochemistry. Here test whether full mevalonate pathway reconstituted vitro used produce commodity chemical isoprene. We construct that uses carbon ATP produced from glycolysis intermediate phosphoenolpyruvate run pathway....
Isoprenoids make up a remarkably diverse class of more than 25000 biomolecules that include familiar compounds such as cholesterol, chlorophyll, vitamin A, ubiquinone, and natural rubber. The two essential building blocks all isoprenoids, isopentenyl pyrophosphate (IPP) dimethylallyl (DMAPP), are ubiquitous in the three domains life. In most eukaryotes archaea, IPP DMAPP generated through mevalonate pathway. We have identified novel enzymes, mevalonate-3-kinase...
Extreme acidophiles are capable of growth at pH values near zero. Sustaining life in acidic environments requires extensive adaptations membranes, proton pumps, and DNA repair mechanisms. Here we describe an adaptation a core biochemical pathway, the mevalonate extreme acidophiles. Two previously known pathways involve ATP dependent decarboxylation either 5-phosphate or 5-pyrophosphate, which single enzyme carries out two essential steps: (1) phosphorylation moiety 3-OH position (2)...
In animals, cholesterol is made from 5-carbon building blocks produced by the mevalonate pathway. Drugs that inhibit pathway such as atorvastatin (lipitor) have led to successful treatments for high in humans. Another potential target inhibition of synthesis diphosphate decarboxylase (MDD), which catalyzes phosphorylation (R)-mevalonate diphosphate, followed decarboxylation yield isopentenyl pyrophosphate. We recently discovered an MDD homolog, mevalonate-3-kinase (M3K) Thermoplasma...
Mevalonate 3,5-bisphosphate decarboxylase is involved in the recently discovered Thermoplasma-type mevalonate pathway. The enzyme catalyzes elimination of 3-phosphate group from as well concomitant decarboxylation substrate. This entire reaction resembles latter half-reactions its homologs, diphosphomevalonate and phosphomevalonate decarboxylase, which also catalyze ATP-dependent phosphorylation 3-hydroxyl their substrates. However, crystal structure structural reasons difference between...