A5011873942- Neuroblastoma Research and Treatments
- Sarcoma Diagnosis and Treatment
- Virus-based gene therapy research
- RNA Interference and Gene Delivery
- Cancer, Hypoxia, and Metabolism
- Nerve injury and regeneration
- Cell death mechanisms and regulation
- Pluripotent Stem Cells Research
- Lung Cancer Research Studies
- Central Venous Catheters and Hemodialysis
- Chemokine receptors and signaling
- Appendicitis Diagnosis and Management
- Intraperitoneal and Appendiceal Malignancies
- Vascular anomalies and interventions
- Cancer Cells and Metastasis
- Cardiac tumors and thrombi
- Surgical site infection prevention
- Vascular Procedures and Complications
- Renal and related cancers
- Intestinal Malrotation and Obstruction Disorders
- Neurogenetic and Muscular Disorders Research
- PARP inhibition in cancer therapy
- Cancer therapeutics and mechanisms
- interferon and immune responses
- Hematopoietic Stem Cell Transplantation
University of Lausanne
1997-2022
University Hospital of Lausanne
2003-2016
Hôpital Orthopédique de la Suisse Romande
2008-2014
Hôpital de l'enfance
2013
Swiss Paediatric Oncology Group
2008
Ball (France)
2008
Institut Gustave Roussy
2003-2004
Temple University
1991
Abstract Ewing's sarcoma family tumors (ESFT) express the EWS-FLI-1 fusion gene generated by chromosomal translocation t(11;22)(q24;q12). Expression of protein in a permissive cellular environment is believed to play key role ESFT pathogenesis. However, induces growth arrest or apoptosis differentiated primary cells, and identity human cells that can support its expression function has until now remained elusive. Here we show mesenchymal stem (hMSC) not only stably maintained without...
Abstract Cancer stem cells that display tumor-initiating properties have recently been identified in several distinct types of malignancies, holding promise for more effective therapeutic strategies. However, evidence such sarcomas, which include some the most aggressive and therapy-resistant tumors, has not shown to date. Here, we identify characterize cancer Ewing's sarcoma family tumors (ESFT), a highly pediatric malignancy believed be mesenchymal cell (MSC) origin. Using magnetic bead...
Cancer stem cells (CSCs) display plasticity and self-renewal properties reminiscent of normal tissue cells, but the events responsible for their emergence remain obscure. We recently identified CSCs in Ewing sarcoma family tumors (ESFTs) showed that they retain mesenchymal cell (MSC) plasticity. In present study, we addressed mechanisms underlie ESFT CSC development. show EWS-FLI-1 fusion gene, associated with 85%–90% ESFTs believed to initiate pathogenesis, induces expression embryonic...
To evaluate the impact of surgeon-assessed extent primary tumor resection on local progression and survival in patients International Society Pediatric Oncology Europe Neuroblastoma Group High-Risk 1 trial.Patients recruited between 2002 2015 with stage 4 disease > year or 4/4S MYCN amplification < who had completed induction without progression, achieved response criteria for high-dose therapy (HDT), no before were included. Data collected excision, severe operative complications, outcome.A...
Huntington's disease (HD) is an inherited disorder characterized by cognitive impairments, motor deficits, and progressive dementia. These symptoms result from neurodegenerative changes mainly affecting the neostriatum. This pathology fatal in 10 to 20 years there currently no treatment for HD. Early course of disease, initial clinical manifestations are due striatal neuronal dysfunction, which later followed massive death. A major therapeutic objective therefore reverse dysfunction prior...
Background Chromaffin cells from the adrenal gland secrete a mixture of compounds that have strong analgesic effect, especially when administered intrathecally. Many studies in animal models shown discordant xenogeneic cell isolates, including chromaffin cells, can survive and biologic effects transplanted within semipermeable membrane capsule. Methods To evaluate clinical potential encapsulated therapy, human-scale implant containing bovine was developed, characterized, implanted...
The Wnt pathway is abnormally activated in the majority of colorectal cancers, and significant knowledge has been gained understanding its role tumor initiation. However, mechanisms metastatic outgrowth cancer remain a major challenge. We report that autophagy-dependent metabolic adaptation survival cells regulated by target oncogenic signaling, homeobox transcription factor PROX1, expressed subpopulation colon progenitor/stem cells. identify direct PROX1 genes show repression pro-apoptotic...
Aebischer, P; Buchser, E; Joseph, J M; Favre, J; de Tribolet, N; Lysaght, Rudnick, S; Goddard, M Author Information
The gene therapy approach presented in this protocol employs a polymer encapsulated, xenogenic, transfected cell line to release human ciliary neurotrophic factor (hCNTF) for the treatment of Amyotrophic Lateral Sclerosis (ALS). A tethered device, containing around 106 genetically modified cells surrounded by semipermeable membrane, is implanted intrathecally; it provides slow continuous hCNTF at rate 0.25 1.0 μg/24 hours. membrane prevents immunologic rejection and interposes physical,...
BACKGROUND The somatostatin analogue, ectrootide, is being used to treat postprandial hypotension in patients with autonomic neuropathy. Although the therapeutic effect of drug presumably secondary a splanchnic vasoconstrictor action, its on hemodynamics has never been characterized Moreover, it unknown whether octreotide acts other vascular beds this group or affects cardiac output. We, therefore, measured splanchnic, forearm, and systemic resistance output before after administering (0.4...
Neuroblastoma (NB) is a neural crest-derived childhood tumor characterized by remarkable phenotypic diversity, ranging from spontaneous regression to fatal metastatic disease. Although the cancer stem cell (CSC) model provides trail characterize cells responsible for onset, NB tumor-initiating (TIC) has not been identified. In this study, relevance of CSC in was investigated taking advantage typical functional characteristics. A predictive association established between self-renewal, as...
Neuroblastoma (NB) is a typical childhood and heterogeneous neoplasm for which efficient targeted therapies high-risk tumors are not yet identified. The chemokine CXCL12, its receptors CXCR4 CXCR7 have been involved in tumor progression dissemination. While expression associated to undifferentiated poor prognosis, the role of CXCR7, recently identified second CXCL12 receptor, has elucidated NB. In this report, expressions were evaluated using tissue micro-array including 156 primary 56...
The successful targeting of neuroblastoma (NB) by associating tumor-initiating cells (TICs) is a major challenge in the development new therapeutic strategies. subfamily aldehyde dehydrogenases 1 (ALDH1) isoenzymes, which comprises ALDH1A1, ALDH1A2, and ALDH1A3, involved synthesis retinoic acid, has been identified as functional stem cell markers diverse cancers. By combining serial neurosphere passages with gene expression profiling, we have previously ALDH1A2 ALDH1A3 potential NB TICs...
Neurotrophic factors hold promise for the treatment of neurodegenerative diseases. Intrathecal transplantation polymer encapsulated cell lines genetically engineered to release neurotrophic provides a means deliver them continuously behind blood–brain barrier. Long-term delivery, however, may benefit from use conditionally mitotic cells avoid overgrowth observed with dividing lines. Myoblast have all advantages lines, i.e., unlimited availability, possibility in vitro screening presence...
Chromaffin cells have been shown to release a combination of pain-reducing neuroactive compounds including catecholamines and opioid peptides. The allogeneic transplantation chromaffin in the subarachnoid space has alleviate pain various rodent models possibly terminal cancer patients. Because shortage human cadaver donor tissue, we are investigating possibility transplanting xenogeneic polymer capsules. In this technique, surrounded by permselective synthetic membrane whose pores suitably...
Neuroblastoma (NB) is a heterogeneous, and particularly malignant childhood neoplasm in its higher stages, with propensity to form metastasis selected organs, particular liver bone marrow, for which there still no efficient treatment available beyond surgery. Recent evidence indicates that the CXCR4/CXCL12 chemokine/receptor axis may be involved promoting NB invasion metastasis. In this study, we explored potential role of CXCR4 behaviour NB, using combination vitro functional analyses vivo...
Abstract Background Histone deacetylase inhibitors (HDACi) are a new class of promising anti-tumour agent inhibiting cell proliferation and survival in tumour cells with very low toxicity toward normal cells. Neuroblastoma (NB) is the second most common solid children still associated poor outcome higher stages and, thus NB strongly requires novel treatment modalities. Results We show here that HDACi Sodium Butyrate (NaB), suberoylanilide hydroxamic acid (SAHA) Trichostatin A (TSA) reduce...
The anaplastic lymphoma kinase (ALK) gene is overexpressed, mutated or amplified in most neuroblastoma (NB), a pediatric neural crest-derived embryonal tumor. two frequent mutations, ALK-F1174L and ALK-R1275Q, contribute to NB tumorigenesis mouse models, cooperate with MYCN the oncogenic process. However, precise role of activating ALK mutations ALK-wt overexpression tumor initiation needs further clarification. Human ALK-wt, ALK-F1174L, ALK-R1275Q were stably expressed murine crest...
Neuroblastoma (NB) is one of the most deadly solid tumors young child, for which new efficient and targeted therapies are strongly needed. The CXCR4/CXCR7/CXCL12 chemokine axis has been involved in progression organ-specific dissemination various cancers. In NB, CXCR4 expression was shown to be associated highly aggressive undifferentiated tumors, while CXCR7 detected more differentiated mature neuroblastic tumors. As investigated vivo, using an orthotopic model tumor cell implantation...
Abstract Background Neuroblastoma (NB) is the second most common solid childhood tumour, an aggressive disease for which new therapeutic strategies are strongly needed. Tumour necrosis factor-related apoptosis-inducing ligand (TRAIL) selectively induces apoptosis in tumour cells, but not normal tissues and therefore represents a valuable candidate therapies. Caspase-8 silenced subset of highly malignant NB results full TRAIL resistance. In addition, despite constitutive caspase-8 expression,...
Objective To investigate, during endotoxic shock, the effect of a treatment norepinephrine (NE) administration on distribution blood flow and adenosine triphosphate (ATP) content in intestinal wall. Design Randomized controlled trial. Setting Animal laboratory. Subjects Domestic pigs. Intervention A total 18 pigs were anesthetized with ketamine pentobarbital, mechanically ventilated, hemodynamically monitored, then challenged continuous infusion Escherichia coli endotoxin (ET) (15 μg/kg) for...