A5043122280- Genetics, Aging, and Longevity in Model Organisms
- Neurobiology and Insect Physiology Research
- Circadian rhythm and melatonin
- Insect Utilization and Effects
- Physiological and biochemical adaptations
- Fungal and yeast genetics research
- Microbial Metabolic Engineering and Bioproduction
- Adipose Tissue and Metabolism
- Birth, Development, and Health
- Nutrition, Genetics, and Disease
- Diet and metabolism studies
- Insect and Arachnid Ecology and Behavior
- Animal Behavior and Reproduction
- Invertebrate Immune Response Mechanisms
- Plant and animal studies
- Insect behavior and control techniques
- Suicide and Self-Harm Studies
- Insect and Pesticide Research
- Dietary Effects on Health
- RNA Research and Splicing
- Mitochondrial Function and Pathology
- Insect Resistance and Genetics
- CRISPR and Genetic Engineering
- Hepatitis C virus research
- Environmental Toxicology and Ecotoxicology
Monash University
2016-2025
Australian Regenerative Medicine Institute
2021
University College London
2009-2018
MRC Unit for Lifelong Health and Ageing
2008-2018
UCL Australia
2007-2011
Max Planck Society
2009-2010
University of Manchester
2009
Delft University of Technology
2002-2007
University of Zurich
2005
Cancer Research UK
2005
The insulin/insulin-like growth factor-like signaling pathway, present in all multicellular organisms, regulates diverse functions including growth, development, fecundity, metabolic homeostasis, and lifespan. In flies, ligands of the Drosophila insulin-like peptides, regulate hemolymph carbohydrate homeostasis during development are expressed a stage- tissue-specific manner. Here, we show that ablation peptide-producing median neurosecretory cells brain leads to increased fasting glucose...
OBJECTIVE--To assess the preference of terminally ill patients with cancer for their place final care. DESIGN--Prospective study randomly selected from hospital and community who were expected to die within a year. Patients live less than two months interviewed at week intervals; otherwise monthly. Their main carer was three after patient9s death. SETTING--District general hospital, hospices, patients9 homes. MAIN OUTCOME MEASURE--Stated preferred care; actual death; reason admission those...
Recent evidence suggests that alterations in insulin/insulin-like growth factor 1 (IGF1) signaling (IIS) can increase mammalian life span. For example, several mouse mutants, impairment of the hormone (GH)/IGF1 axis increases span and also insulin sensitivity. However, intracellular route to altered aging remains unclear. We therefore measured mice lacking either receptor substrate (IRS) or 2, major effectors IIS receptors. Our provisional results indicate female Irs1-/- are long-lived....
Dietary restriction (DR) extends life span in diverse organisms, including mammals, and common mechanisms may be at work. DR is often known as calorie restriction, because it has been suggested that reduction of calories, rather than particular nutrients the diet, mediates extension rodents. We here demonstrate by Drosophila not attributable to intake. Reduction either dietary yeast or sugar can reduce mortality extend span, but an amount unrelated content food, with having a much greater...
Abstract Dietary restriction (DR) extends life span in many organisms, through unknown mechanisms that may or not be evolutionarily conserved. Because different laboratories use diets and techniques for implementing DR, the outcomes strictly comparable. This complicates intra- interspecific comparisons of DR is therefore central to model organisms research this topic. Drosophila melanogaster an important study but nutritional content its diet typically poorly defined. We have compared fly...
Choosing the right nutrients to consume is essential health and wellbeing across species. However, factors that influence these decisions are poorly understood. This particularly true for dietary proteins, which important determinants of lifespan reproduction. We show in Drosophila melanogaster, amino acids (eAAs) concerted action commensal bacteria Acetobacter pomorum Lactobacilli critical modulators food choice. Using a chemically defined diet, we absence any single eAA from diet...
Measurement of food intake in the fruit fly Drosophila melanogaster is often necessary for studies behaviour, nutrition and drug administration. There no reliable agreed method measuring flies undisturbed, steady state, normal culture conditions. We report such a method, based on measurement feeding frequency by proboscis-extension, validated short-term measurements dye intake. used to demonstrate that (a) female feed more frequently than males, (b) when housed larger groups (c) varies at...
Profiles of genome-wide transcriptional events for a given environmental condition can be importance in the diagnosis poorly defined environments. To identify clusters genes constituting such diagnostic profiles, we characterized specific responses Saccharomyces cerevisiaeto growth limitation by carbon, nitrogen, phosphorus, or sulfur. Microarray experiments were performed using cells growing steady-state conditions chemostat cultures at same dilution rate. This enabled us to study effects...
Methods: 203 men were interviewed from 15 prisons, comprising one‐fifth of all sentenced in this age group England and Wales. Assessment included semi‐structured interviews covering chronic acute health problems, recording major illnesses the medical notes prison reception screen.
Assessment of reproducibility DNA-microarray analysis from published data sets is complicated by the use different microbial strains, cultivation techniques, and analytical procedures. Because intra- interlaboratory highly relevant for application in functional genomics metabolic engineering, we designed a set experiments to specifically address this issue. Saccharomyces cerevisiae CEN.PK113-7D was grown under defined conditions glucose-limited chemostats, followed transcriptome with...
Abstract Background To what extent are the determinants of aging in animal species universal? Insulin/insulin-like growth factor (IGF)-1 signaling (IIS) is an evolutionarily conserved (public) regulator longevity; yet it remains unclear whether genes and biochemical processes through which IIS acts on public or private (that is, lineage specific). address this, we have applied a novel, multi-level cross-species comparative analysis to compare gene expression changes accompanying increased...
ABSTRACT The absence of alcoholic fermentation makes pyruvate decarboxylase-negative (Pdc − ) strains Saccharomyces cerevisiae an interesting platform for further metabolic engineering central metabolism. However, Pdc S. have two growth defects: (i) on synthetic medium in glucose-limited chemostat cultures requires the addition small amounts ethanol or acetate and (ii) even presence a C 2 compound, these cannot grow batch with glucose. We used subsequent phenotypic selection strategies to...