A5019386577- MicroRNA in disease regulation
- Plant Reproductive Biology
- RNA Interference and Gene Delivery
- Extracellular vesicles in disease
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Antifungal resistance and susceptibility
- Phytochemical Studies and Bioactivities
- CRISPR and Genetic Engineering
- Magnetic Properties and Applications
- Mosquito-borne diseases and control
- Plant Virus Research Studies
- Circular RNAs in diseases
- Chromosomal and Genetic Variations
- Medicinal Plant Research
- Pharmacology and Nanomedicine Research
University of Delaware
2018-2024
Vellore Institute of Technology University
2017
The origin and early evolution of sex chromosomes have been hypothesized to involve the linkage factors with antagonistic effects on male female function. Garden asparagus (Asparagus officinalis) is an ideal species investigate this hypothesis, as X Y are cytologically homomorphic evolved from ancestral autosome pair in association a shift hermaphroditism dioecy. Mutagenesis screens paired single-molecule fluorescence situ hybridization directly implicate Y-specific genes that respectively...
Significance Alphaviruses are mosquito-borne RNA viruses that cause rash, arthritis, and neurologic disease. Despite a continued risk of outbreaks, there no licensed interventions for any alphavirus. For progress in control, an understanding the molecular targets affect virus replication virulence is essential. This paper characterizes conserved macrodomain factor nonstructural protein 3 (nsP3). We discovered ADP-ribosylhydrolase activity critical controlling composition cellular...
Circular RNAs (circRNAs) are regulatory which have recently been shown to clinical significance in several diseases, including, but not limited to, various cancers, neurological diseases and cardiovascular diseases. The function of such is largely dependent on their subcellular localization. Several circRNAs conduct antagonistic roles compared the products linear isoforms, thus need be characterized distinctly from RNAs. However, conventional fluorescent situ hybridization (FISH) techniques...
The individual detection of human immunodeficiency virus (HIV) virions and resolution from extracellular vesicles (EVs) during analysis is a difficult challenge. Infectious enveloped nonviral EVs are released simultaneously by HIV-infected host cells, in addition to hybrid viral containing combinations HIV components but lacking replicative ability. Complicating the issue, both delimited lipid bilayer share similar size density. feature that distinguishes infectious genomic RNA (gRNA), which...
The origin of sex chromosomes has been hypothesized to involve the linkage factors with antagonistic effects on male and female function. Garden asparagus (Asparagus officinalis L.) is an ideal species test this hypothesis, as X Y are cytologically homomorphic recently evolved from ancestral autosome pair in association a shift hermaphroditism dioecy. Mutagenesis screens paired single-molecule fluorescence situ hybridization (smFISH) directly implicate Y-specific genes that respectively...
Abstract Interest in using nanoparticles for delivery of therapeutic RNA has been steadily growing, provoking a need to precisely understand their structure and contents. Single-particle single-molecule analysis techniques provide snapshots single biological nanoparticles, including viruses, liposomes, extracellular vesicles (EVs). While existing methods primarily focus on protein detection, is becoming increasingly prevalent. A method simultaneously detect internal the same particle would...