A5100708107- Pancreatic function and diabetes
- Diabetes and associated disorders
- Diabetes Management and Research
- Diabetes Treatment and Management
- Cardiomyopathy and Myosin Studies
- Muscle Physiology and Disorders
- Immune Cell Function and Interaction
- Endoplasmic Reticulum Stress and Disease
- Neurogenetic and Muscular Disorders Research
- T-cell and B-cell Immunology
- Genetics and Neurodevelopmental Disorders
- Cellular Mechanics and Interactions
- Diet, Metabolism, and Disease
- Gut microbiota and health
- Virus-based gene therapy research
- Connexins and lens biology
- CAR-T cell therapy research
- Metabolomics and Mass Spectrometry Studies
- Ginseng Biological Effects and Applications
- Immunotherapy and Immune Responses
- Regulation of Appetite and Obesity
- Zebrafish Biomedical Research Applications
- Heat shock proteins research
- Phosphodiesterase function and regulation
- Allergic Rhinitis and Sensitization
Ganzhou People's Hospital
2025
University of Massachusetts Chan Medical School
2009-2019
Rensselaer Polytechnic Institute
2005-2010
Diabetes Australia
2010
Institute of Molecular Medicine
2010
Human pluripotent stem cells (hPSCs) represent a renewable source of pancreatic beta for both basic research and therapeutic applications. Given this outstanding potential, significant efforts have been made to identify the signaling pathways that regulate development in hPSC differentiation cultures. In study, we demonstrate combination epidermal growth factor (EGF) nicotinamide induces generation NKX6-1+ progenitors from all lines tested. Furthermore, show size population is regulated by...
Abstract The mannose receptor (MR) is a C-type lectin expressed by dendritic cells (DCs). We have investigated the ability of MR to recognize glycosylated allergens. Using gene silencing strategy, we specifically inhibited expression on human monocyte-derived DCs. show that mediates internalization diverse allergens from mite (Der p 1 and Der 2), dog (Can f 1), cockroach (Bla g peanut (Ara h 1) through their carbohydrate moieties. All these bind lectin-like recognition domains 4–7 MR. also...
Type 1 diabetes studies consistently generate data showing islet β-cell dysfunction and T cell–mediated anti-β-cell–specific autoimmunity. To explore the pathogenesis, we interrogated transcriptomes from donors with without type using both bulk-sorted single β-cells. Consistent immunohistological studies, β-cells displayed increased Class I transcripts associated mRNA species. These also expressed for II antigen presentation pathway components, but lacked macrophage marker CD68....
RNA-seq protocols that focus on transcript termini are well suited for applications in which template quantity is limiting. Here we show that, when applied to end-sequencing data, analytical methods designed global produce computational artifacts. To remedy this, created the End Sequence Analysis Toolkit (ESAT). As a test, first compared and bulk using RNA from dendritic cells stimulated with lipopolysaccharide (LPS). predicted by telescripting model transcriptional bursts, ESAT detected an...
OBJECTIVE To create an immunodeficient mouse model that spontaneously develops hyperglycemia to serve as a diabetic host for human islets and stem cell–derived β-cells in the absence or presence of functional immune system. RESEARCH DESIGN AND METHODS We backcrossed Ins2Akita mutation onto NOD-Rag1null IL2rγnull strain determined 1) spontaneous development hyperglycemia, 2) ability islets, dissociated islet cells restore euglycemia, 3) generation system following engraftment hematopoietic...
Gimap5 (GTPase of the immunity-associated protein 5) has been linked to regulation T cell survival, and polymorphisms in human GIMAP5 gene associate with autoimmune disorders. The BioBreeding diabetes-prone (BBDP) rat a mutation that leads spontaneous apoptosis peripheral cells by an unknown mechanism. Because localizes endoplasmic reticulum (ER), we hypothesized absence functional initiates death through disruptions ER homeostasis. We observed increases stress-associated chaperones but not...
Stachydrine (STA) has therapeutic effects on heart disorders. The current study assessed its Type 2 diabetes (T2D) induced cardiac disorders by focusing the heart-gut axis. Mice were subjected to high-fat diet (HFD) and streptozocin (STZ) induce such as inflammation structural deteriorations, which handled with STA. Changes regarding composition metabolism of gastrointestinal (GI) microbiota then determined using a multiomics strategy, including amplicon sequencing metabolomics. data showed...
The pathogenesis of human type 1 diabetes, characterized by immune-mediated damage insulin-producing β-cells pancreatic islets, may involve viral infection. Essential components the innate immune antiviral response, including I interferon (IFN) and IFN receptor-mediated signaling pathways, are candidates for determining susceptibility to diabetes. Numerous aspects diabetes recapitulated in LEW.1WR1 rat model. Diabetes can be induced weanling rats challenged with virus or mimetic...
The adipokine, leptin, regulates blood glucose and the insulin secretory function of beta cells, while also modulating immune cell function. We hypothesized that dual effects leptin may prevent or suppress autoreactive destruction cells in a virally induced rodent model type 1 diabetes. Nearly 100% weanling BBDR rats treated with combination an innate system activator, polyinosinic:polycytidylic acid (pIC), Kilham rat virus (KRV) become diabetic within predictable time frame. utilized this...
Individuals with schizophrenia and their first-degree relatives have higher rates of type 2 diabetes (T2D) than the general population (18–30 vs. 1.2–6.3%), independent body mass index antipsychotic medication, suggesting shared genetic components may contribute to both diseases. The cause this association remains unknown. Mutations in disrupted 1 (DISC1) increase risk developing psychiatric disorders [logarithm (base 10) odds = 7.1]. Here, we identified DISC1 as a major player controlling...
Proteomic profiling of serum is a powerful technique to identify differentially expressed proteins that can serve as biomarkers predictive disease onset. In this study, we utilized two-dimensional (2D) gel analysis followed by matrix-assisted-laser desorption/ionization time-of-flight mass spectrometry putative for autoimmune type 1 diabetes (T1D) in biobreeding resistant (BBDR) rats induced express the disease. Treatment with toll-like receptor 3 ligand, polyinosinic:polycytidilic acid...
Purpose: Dipeptidyl-peptidase-4 (DPP-4) inhibitors are known to increase insulin secretion and beta cell proliferation in rodents. To investigate the effects on human cells vivo, we utilize immunodeficient mice transplanted with islets. The study goal was determine efficacy of alogliptin, a DPP-4 inhibitor, enhance function an vivo context using diabetic engrafted pancreatic Methods: Streptozotocin-induced NOD- scid IL2rγnull (NSG) were adult islets three separate trials. Transplanted...
Studies of human cadaveric pancreas specimens indicate that inflammation plays an important role in type 1 diabetes pathogenesis. Due to the inaccessibility living patients, imaging technology visualize is much need. In this study, we investigated feasibility utilizing ultrasound assess longitudinally rats during progression leading onset.The virus-inducible BBDR rat model was used systematically investigate changes occur prior and development autoimmunity. The nearly 100% incidence upon...
Glucagon-like peptide-1 induces glucose-dependent insulin secretion and, in rodents, increases proliferation and survival of pancreatic beta cells. To investigate the effects on human cells, we used immunodeficient mice transplanted with islets. The goal was to determine whether lixisenatide, a glucagon-like receptor agonist, improves islet function vivo.Five independent transplant studies were conducted islets from five individual donors. Diabetic islet-engrafted treated lixisenatide (50,...
Experiments using isolated pancreatic islets are important for diabetes research, but expensive and of limited abundance. Islets contain a mixed cell population in structured architecture that impacts function, human widely variable type composition. Current frequently used methods to study cultured include molecular studies performed on whole islets, lumping disparate islet types together, or microscopy dispersed cells, disrupting architecture. For vivo studies, paraffin-embedded pancreas...
Epidemiologic and clinical evidence suggests that virus infection plays an important role in human type 1 diabetes pathogenesis. We used the virus-inducible BioBreeding Diabetes Resistant (BBDR) rat to investigate ability of sodium salicylate, a non-steroidal anti-inflammatory drug (NSAID), modulate development diabetes. BBDR rats treated with Kilham (KRV) polyinosinic:polycytidylic acid (pIC, TLR3 agonist) develop at nearly 100% incidence by ~2 weeks. found distinct temporal profiles...