A5080657924- Alzheimer's disease research and treatments
- Trace Elements in Health
- Cholinesterase and Neurodegenerative Diseases
- Molecular Sensors and Ion Detection
- Prion Diseases and Protein Misfolding
- Parkinson's Disease Mechanisms and Treatments
- Hemoglobinopathies and Related Disorders
- Calcium signaling and nucleotide metabolism
- Electrochemical Analysis and Applications
- Endoplasmic Reticulum Stress and Disease
- Cell Adhesion Molecules Research
- Heme Oxygenase-1 and Carbon Monoxide
- Hemoglobin structure and function
- Protein Interaction Studies and Fluorescence Analysis
- Neuroinflammation and Neurodegeneration Mechanisms
- Biochemical effects in animals
- Cancer-related molecular mechanisms research
- Metal complexes synthesis and properties
- Heavy Metal Exposure and Toxicity
- Advanced Nanomaterials in Catalysis
- Supramolecular Self-Assembly in Materials
- Neurological diseases and metabolism
- Computational Drug Discovery Methods
- Angiogenesis and VEGF in Cancer
- Conducting polymers and applications
University of Pavia
2017-2025
Tau protein is present in significant amounts neurons, where it contributes to the stabilization of microtubules. Insoluble neurofibrillary tangles tau are associated with several neurological disorders known as tauopathies, among which Alzheimer's disease. In binds tubulin through its microtubule binding domain comprises four imperfect repeats (R1-R4). The histidine residues contained these fragments potential sites for metal ions and located close regions that drive formation amyloid...
Spontaneous cleavage reactions normally occur in vivo on amino acid peptide backbones, leading to fragmentation products that can have different physiological roles and toxicity, particularly when the substrate of hydrolytic processes are neuronal peptides proteins highly related neurodegeneration. We report a study performed with HPLC-MS technique at temperatures (4 °C 37 °C) fragments (amyloid-β, tau, α-synuclein) conditions presence Cu2+ Zn2+ ions, two metal ions found millimolar...
α-Synuclein (αSyn) constitutes the main protein component of Lewy bodies, which are pathologic hallmark in Parkinson's disease. αSyn is unstructured solution but interaction with lipid membrane modulates its conformation by inducing an α-helical structure N-terminal region. In addition, metal ions can trigger upon binding and/or through metal-promoted generation reactive oxygen species lead to a cascade structural alterations. For these reasons, ternary between αSyn, copper, and membranes...
Tau is a neuronal protein involved in axonal stabilization; however under pathological conditions, it triggers the deposition of insoluble neurofibrillary tangles, which are one biomarkers for Alzheimer's disease. The factors that might influence fibrillation process i) two cysteine residues pseudorepetitive regions, called R2 and R3, can modulate protein–protein interaction via disulfide cross-linking; ii) an increase reactive oxygen species affecting post-translational modification tau;...
The combination between dyshomeostatic levels of catecholamine neurotransmitters and redox-active metals such as copper iron exacerbates the oxidative stress condition that typically affects neurodegenerative diseases. We report a comparative study reactivity complexes with amyloid-β (Aβ40) prion peptide fragment 76-114 (PrP76–114), containing high-affinity binding site, toward dopamine 4-methylcatechol, in aqueous buffer sodium dodecyl sulfate micelles, model membrane environment....
Copper(II) binding to prion peptides does not prevent Cu redox cycling and formation of reactive oxygen species (ROS) in the presence reducing agents. The toxic effects these are exacerbated catecholamines, indicating that dysfunction catecholamine vesicular sequestration or recovery after synaptic release is a dangerous amplifier induced oxidative stress. bound including high affinity site involving histidines adjacent octarepeats exhibits marked catalytic activity toward dopamine...
Exogenous nitric oxide appears a promising therapeutic approach to control cancer progression. Previously, nickel-based nonoate, [Ni(SalPipNONO)], inhibited lung cells, along with impairment of angiogenesis. The Zn(II) containing derivatives [Zn(PipNONO)Cl] exhibited protective effect on vascular endothelium. Here, we have evaluated the antitumor properties in human (A549) and melanoma (A375) cells. Metastasis initiates epithelial-mesenchymal transition (EMT) process, consisting acquisition...
Interaction of copper ions with Aβ peptides alters the redox activity metal ion and can be associated neurodegeneration. Many studies deal characterization binding mode responsible for reactivity. Oxidation experiments dopamine related catechols by copper(II) complexes N-terminal amyloid-β Aβ16 Aβ9, Aβ16[H6A] Aβ16[H13A] mutant forms, both in their free amine N-acetylated forms show that efficient reactivity requires oxygenation a CuI-bis(imidazole) complex bound substrate. Therefore, active...
Abstract Dopamin (DA) ist das wichtigste und häufigste Catecholamin im Gehirn zugleich Vorläufer anderer Neurotransmitter. Die Degeneration von Neuronen des Nigrostriatums (Substantia nigra, Pars compacta) bei Parkinson‐Kranken die am besten untersuchte Verknüpfung zwischen Neurotransmission Neuropathologie durch DA. Catecholamine sind reaktive Moleküle, für es komplexe Kontroll‐ Transportsysteme gibt. Unter normalen Bedingungen werden kleine Mengen cytosolischen Dopamins unter Melanisierung...
Tau is a widespread neuroprotein that regulates the cytoskeleton assembly. In some neurological disorders, known as tauopathies, tau dissociated from microtubule and forms insoluble neurofibrillary tangles. comprises four pseudorepeats (R1-R4), containing one (R1, R2, R4) or two (R3) histidines, potentially act metal binding sites. Moreover, Cys291 Cys322 in R2 R3, respectively, might have an important role protein aggregation, through possible disulfide bond formation, and/or affecting...
The peroxidase activity of hemin-peptide complexes remains a potential factor in oxidative damage relevant to neurodegeneration. Here, we present the effect temperature, ionic strength, and pH pathophysiological conditions on dynamic equilibrium between high-spin low-spin hemin-Aβ40 constructs. This influence was also demonstrated using 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) dopamine (DA) oxidation rate analyses with increasing ratios Aβ16 Aβ40 (up 100 equivalents)....
The redox chemistry of copper(II) is strongly modulated by the coordination to amyloid-β peptides and stability resulting complexes. Amino-terminal copper nickel binding motifs (ATCUN) identified in truncated Aβ sequences starting with Phe4 show very high affinity for ions. Herein, we study oxidase activity [Cu–Aβ4−x] [Cu–Aβ1−x] complexes toward dopamine other catechols. results that CuII–ATCUN site not redox-inert; reduction metal induced catechol occurs through an inner sphere reaction....
A challenge in mimicking tyrosinase activity using model compounds is to reproduce its enantioselectivity. Good enantioselection requires rigidity and a chiral center close the active site. In this study, synthesis of new copper complex, [Cu2(mXPhI)]4+/2+, based on an m-xylyl-bis(imidazole)-bis(benzimidazole) ligand containing stereocenter with benzyl residue directly bound chelating ring, reported. Binding experiments show that cooperation between two metal centers weak, probably due steric...