A5006442217- Skin and Cellular Biology Research
- Single-cell and spatial transcriptomics
- Cancer-related gene regulation
- Genomics and Chromatin Dynamics
- Machine Learning in Bioinformatics
- Genomics and Phylogenetic Studies
- Pluripotent Stem Cells Research
- Cancer Cells and Metastasis
- Protein Structure and Dynamics
- Epigenetics and DNA Methylation
- RNA modifications and cancer
- RNA Research and Splicing
- Endoplasmic Reticulum Stress and Disease
- Chemical Reactions and Isotopes
- Hair Growth and Disorders
- RNA and protein synthesis mechanisms
- Skin Protection and Aging
- Cellular Mechanics and Interactions
Yale University
2022-2025
Dana-Farber Cancer Institute
2025
Cornell University
2022-2024
The conserved WD40-repeat protein WDR5 interacts with multiple proteins both inside and outside the nucleus. However, it is currently unclear whether how distribution of between complexes regulated. Here, we show that an unannotated microprotein EMBOW (endogenous binder WDR5) dually encoded in human SCRIB gene regulates its binding to interaction partners, including KMT2A KIF2A. cell cycle regulated, two expression maxima at late G1 phase G2/M phase. Loss decreases KIF2A, aberrantly shortens...
Abstract Thousands of short open reading frames (sORFs) are translated outside annotated coding sequences. Recent studies have pioneered searching for sORF-encoded microproteins in mass spectrometry (MS)-based proteomics and peptidomics datasets. Here, we assessed literature-reported MS-based identifications unannotated human proteins. We find that vary by three orders magnitude the number proteins they report. Of nearly 10,000 reported peptides, 96% were unique to a single study, 12% mapped...
Abstract There is growing evidence that microorganisms are an important component of the tumor microenvironment. However, conflicting reports regarding cancer microbiome have left extent microbial presence across types unclear, highlighting need for more accurate methods profiling tumor-associated microorganisms. Leveraging new human genome T2T-CHM13, we developed a host-subtraction and decontamination pipeline accurately in sequencing data benchmarked it on simulated real-world datasets. We...
ABSTRACT Transit-amplifying (TA) cells are progenitors that undergo an amplification phase followed by transition into extinction phase. A long postulated epidermal TA progenitor with biphasic behavior has not yet been experimentally observed in vivo. Here, we identify such a population using clonal analysis of Aspm-CreER genetic cell-marking mice, which uncovers contribution to both homeostasis and injury repair adult skin. This is more frequently dividing than Dlx1-CreER-marked long-term...
Over the past 15 years, hundreds of previously undiscovered bacterial small open reading frame (sORF)-encoded polypeptides (SEPs) fewer than fifty amino acids have been identified, and biological functions ascribed to an increasing number SEPs from intergenic regions RNAs. However, despite numbering in dozens
Abstract Adult interfollicular epidermis (IFE) renewal is likely orchestrated by physiological demands of its complex tissue architecture comprising spatial and cellular heterogeneity. Mouse tail back skin display two kinds basal IFE domains that regenerate at different rates. Here we elucidate the molecular states marker expression single cell transcriptomics in mouse human skin. We uncover paths differentiation reflect part domain organization. unravel previously unrecognized similarities...
Abstract Transit-amplifying progenitor populations with phased behavior have long been postulated as essential to epidermal renewal, but not experimentally observed in vivo . Here we identify a population bi-phasic using CreER genetic cell-marking mice for long-term lineage tracing and clonal analysis. Nascent, highly expressing Aspm cells undergo an amplification-phase followed by timed transition into extinction-phase, near complete loss of descending from skin. Generalized birth-death...
The highly conserved WD40-repeat protein WDR5 interacts with multiple proteins both inside and outside the nucleus, including MLL/SET1 histone methyltransferases that catalyze H3 lysine 4 (H3K4) di- tri-methylation (me2,3), KIF2A, a member of Kinesin-13 family microtubule depolymerases. However, it is currently unclear whether, how, distribution between complexes regulated. Here, we show an unannotated microprotein dually encoded in human SCRIB gene regulates its binding to epigenetic KIF2A....