A5064385767- Retinal Development and Disorders
- Neuroscience and Neuropharmacology Research
- Prion Diseases and Protein Misfolding
- Cell death mechanisms and regulation
- Glaucoma and retinal disorders
- Neurological diseases and metabolism
- Retinopathy of Prematurity Studies
- Nerve injury and regeneration
- Virus-based gene therapy research
- Alzheimer's disease research and treatments
- Retinal Diseases and Treatments
- Photoreceptor and optogenetics research
- Neurogenesis and neuroplasticity mechanisms
- Autophagy in Disease and Therapy
- RNA regulation and disease
- Retinoids in leukemia and cellular processes
- Mitochondrial Function and Pathology
- Neuroinflammation and Neurodegeneration Mechanisms
- RNA Interference and Gene Delivery
- CRISPR and Genetic Engineering
- Receptor Mechanisms and Signaling
- Cancer-related Molecular Pathways
- Visual perception and processing mechanisms
- Connexins and lens biology
- Neural dynamics and brain function
Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro
1996-2025
Universidade Federal do Rio de Janeiro
2016-2025
Universidade Feevale
2018-2023
Instituto de Biofísica Carlos Chagas Filho
2003-2022
Universidade Federal do Rio Grande do Sul
2007-2021
Pontifícia Universidade Católica do Rio Grande do Sul
2021
Fundação Carlos Chagas
2021
Clinique Claude-Bernard
2015
Instituto di Biofisica
2015
Czech Academy of Sciences, Institute of Biophysics
2011
Abstract The number of ganglion cells in the retina postnatal rat has been examined. We estimated both axons optic nerve and which can be retrogradely labelled with horseradish peroxidase from injections into brain. In newborn there are at least twice as many adult rat. By retrograde labelling following transection their 24‐48 hrs later we find no evidence that withdraw axon without degeneration parent cell body. have found excess lost over first ten days during this period observe pyknotic...
Understanding the physiological function of cellular prion (PrPc) depends on investigation PrPc-interacting proteins. Stress-inducible protein 1 (STI1) is a specific PrPc ligand that promotes neuroprotection retinal neurons through cAMP-dependent kinase A (PKA). Here, we examined signaling pathways and functional consequences interaction with STI1 in hippocampal neurons. Both are abundantly expressed highly colocalized hippocampus situ, indicating they can interact vivo. Recombinant...
Plant defensins, components of the plant innate immune system, are cationic cysteine-rich antifungal peptides. Evidence from literature [Thevissen, K., et al. (2003) Peptides 24, 1705−1712] has demonstrated that patches fungi membrane containing mannosyldiinositolphosphorylceramide and glucosylceramides selective binding sites for defensins isolated Dahlia merckii Raphanus sativus, respectively. Whether interact directly or indirectly with fungus intracellular targets is unknown. To identify...
The prion glycoprotein (PrPC) is mostly located at the cell surface, tethered to plasma membrane through a glycosyl-phosphatydil inositol anchor. Misfolding of PrPC associated with transmissible spongiform encephalopathies, whereas its normal conformer serves as receptor for oligomers β-amyloid peptide, which play major role in pathogenesis Alzheimer's Disease. highly expressed both nervous and immune systems, well other organs, but functions are controversial. Extensive experimental work...
The prion protein (PrPC) is highly expressed in the nervous system, and its abnormal con-former associated with diseases. PrPC anchored to cell membranes by glycosylphosphatidylinositol, transmembrane proteins are likely required for PrPC-mediated intracellular signaling. Binding of laminin (Ln) modulates neuronal plasticity memory. We addressed signaling pathways triggered PrPC-Ln interaction order identify involved transduction signals. Ln γl-chain peptide, which contains binding site...
During development of the retina, programmed cell death helps to establish final size and distribution various classes in distinct layers tissue. Here we show that dying cells developing ganglion inner nuclear are clustered spatially gap junction inhibitors decrease clustering cells. To confirm role junctions death, induced targeted via intracellular cytochrome c (C ) examined their neighbors for apoptotic morphology or caspase-3 cleavage. These studies indicate bystander killing extends...
We investigated the role of Fas ligand in murine silicosis. Wild-type mice instilled with silica developed severe pulmonary inflammation, local production tumor necrosis factor (TNF)-α, and interstitial neutrophil macrophage infiltration lungs. Strikingly, ligand–deficient generalized lymphoproliferative disease mutant (gld) did not develop The gld had markedly reduced extravasation into bronchoalveolar space, show increased TNF-α production, nor inflammation. Bone marrow chimeras adoptive...
Abstract The cellular prion protein (PrPc) is a glycoprotein anchored by glycosylphosphatidylinositol (GPI) to the cell surface and abundantly expressed in central nervous system. It also variety of types immune We investigated role PrPc phagocytosis apoptotic cells other particles. Macrophages from mice with deletion Prnp gene showed higher rates than wild-type macrophages vitro assays. elimination GPI-anchored proteins rendered these as efficient derived knockout mice. In situ detection...
The secreted cochaperone STI1 triggers activation of protein kinase A (PKA) and ERK1/2 signaling by interacting with the cellular prion (PrP<sup>C</sup>) at cell surface, resulting in neuroprotection increased neuritogenesis. Here, we investigated whether PrP<sup>C</sup> trafficking tested this process controls PrP<sup>C</sup>-dependent signaling. We found that STI1, but not a mutant unable to bind PrP<sup>C</sup>, induced endocytosis. STI1-induced did occur cells devoid endogenous...
Abstract Gliomas are tumors derived from glia or their precursors within the central nervous system. Clinically, gliomas divided into four grades and glioblastoma multiforme (GBM), also referred as grade IV astrocytoma, is most aggressive common glioma in humans. The prognosis for patients with GBM remains dismal, a median survival of 9–12 months. Despite striking heterogeneity, alterations specific cellular signal transduction pathways occur GBMs. Previous work our group identified...