A5088819676- RNA Research and Splicing
- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- Genetics and Neurodevelopmental Disorders
- MicroRNA in disease regulation
- Graphene and Nanomaterials Applications
- Cancer-related gene regulation
- Mercury impact and mitigation studies
- Graphene research and applications
- Recycling and utilization of industrial and municipal waste in materials production
- Concrete and Cement Materials Research
- Cardiomyopathy and Myosin Studies
- Clay minerals and soil interactions
- Carbon Nanotubes in Composites
University of Manitoba
2022-2024
Florida International University
2024
Mammalian cardiac troponin I (cTnI) contains a highly conserved amino-terminal extension harboring protein kinase A targets [serine-23 and -24 (Ser 23/24 )] that are phosphorylated during β-adrenergic stimulation to defend diastolic filling by means of an increased cardiomyocyte relaxation rate. In this work, we show the Ser -encoding exon 3 TNNI3 was pseudoexonized multiple times in shrews moles mimic phosphorylation without adrenergic stimulation, facilitating evolution exceptionally high...
Following phytoremediation, the disposal of accumulating plants (phytoaccumulators) is challenging because accumulated metals could leach back into soil if not properly managed. Therefore, this study aims to use calcined clay (CC)-based geopolymer stabilize Pb, Cu, and Zn in a phytoaccumulator (Sporobolus pyramidalis) ash (PA). Additionally, effect adding PA on setting time, mechanical heavy leaching properties geopolymers was investigated, determine their environmental suitability potential...
Pairing of splice sites across an intron or exon is the central point definition in pre-mRNA splicing with latter mode proposed for most mammalian exons. However, transcriptome-wide pairing within endogenous transcripts has not been examined prevalence each cells. Here we report such pairings rat GH3 pituitary cells by measuring relative abundance nuclear RNA-Seq reads at start end (RISE). Interestingly, RISE indexes are positively correlated between 5′ and 3′ specifically introns exons but...
Abstract As an inhibitor of DNA methyltransferases (DNMT) and anti-cancer drug, the many effects 5-aza-cytidine (5-azaC) on gene expression remains unknown. Here we show that 5-azaC treatment cultured pituitary tumour cells increases relative usage genomic terminal exons (GTE) across transcriptome. This effect is largely achieved by switching mRNA polyadenylation (poly(A)) from proximal sites to GTE, which have a more optimal poly(A) signal consensus motif. Consistently, upregulates...
Abstract Interval-training activities induce adaptive cellular changes without altering their fundamental identity, but the precise underlying molecular mechanisms are not fully understood. In this study, we demonstrate that interval-training depolarization (ITD) of pituitary cells triggers distinct or homeostatic splicing responses alternative exons. This occurs while preserving steady-state expression Prolactin and other hormone genes. The nature these depends on exon’s DNA methylation...
Abstract Interval-training activities induce adaptive cellular changes without altering their fundamental identity, but the precise underlying molecular mechanisms are not fully understood. In this study, we demonstrate that interval-training depolarization (ITD) of pituitary cells triggers distinct or homeostatic splicing responses alternative exons. This occurs while preserving steady-state expression Prolactin and other hormone genes. The nature these depends on exon's DNA methylation...