A5015359141- Photosynthetic Processes and Mechanisms
- Protist diversity and phylogeny
- Algal biology and biofuel production
- Genomics and Phylogenetic Studies
- Chromosomal and Genetic Variations
- ATP Synthase and ATPases Research
- Mitochondrial Function and Pathology
- Photoreceptor and optogenetics research
- Aquatic Ecosystems and Phytoplankton Dynamics
- Fungal and yeast genetics research
- DNA Repair Mechanisms
- Aquaculture Nutrition and Growth
- Spectroscopy and Quantum Chemical Studies
- Marine and coastal ecosystems
- Heat shock proteins research
- Fish biology, ecology, and behavior
- Light effects on plants
- Advanced Thermodynamics and Statistical Mechanics
- Evolution and Genetic Dynamics
Sorbonne Université
2019-2024
Centre National de la Recherche Scientifique
2017-2024
Laboratoire de Biologie Computationnelle et Quantitative
2020-2024
Institut de Biologie Physico-Chimique
2019-2023
Rudjer Boskovic Institute
2023
Institut de Biologie Paris-Seine
2020-2023
Institut de Biosciences et Biotechnologies
2017-2022
Biologie du Chloroplaste et Perception de la Lumière chez les Microalgues
2019
CEA Cadarache
2015-2017
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2015-2017
During oxygenic photosynthesis, the reducing power generated by light energy conversion is mainly used to reduce carbon dioxide. In bacteria and archae, flavodiiron (Flv) proteins catalyze O2 or NO reduction, thus protecting cells against oxidative nitrosative stress. These are found in cyanobacteria, mosses, microalgae, but have been lost angiosperms. Here, we chlorophyll fluorescence oxygen exchange measurement using [18O]-labeled a membrane inlet mass spectrometer characterize...
Biochemical processes in chloroplasts are important for virtually all life forms. Tight regulation of protein homeostasis and the coordinated assembly complexes, composed both imported locally synthesized subunits, vital to plastid functionality. Protein biogenesis requires action cotranslationally acting molecular chaperones. One such chaperone is trigger factor (TF), which known bind most newly proteins bacteria, thereby assisting their correct folding maturation. However, how these...
The current Chlamydomonas reinhardtii reference genome remains fragmented due to gaps stemming from large repetitive regions. To overcome the vast majority of these gaps, publicly available Oxford Nanopore Technology data were used create a new reference-quality de novo assembly containing only 21 contigs, 30/34 telomeric ends, and size 111 Mb.
Abstract In most eukaryotes, subtelomeres are dynamic genomic regions populated by multi-copy sequences of different origins, which can promote segmental duplications and chromosomal rearrangements. However, their repetitive nature has complicated the efforts to sequence them, analyse structure infer how they evolved. Here, we use recent genome assemblies Chlamydomonas reinhardtii based on long-read sequencing comprehensively describe subtelomere architecture 17 chromosomes this model...
Recent advances in long-read sequencing technologies have enabled the complete assembly of eukaryotic genomes from telomere to by allowing repeated regions be fully sequenced and assembled, thus filling gaps left previous short-read methods. Furthermore, can also help characterizing structural variants, with applications fields genome evolution or cancer genomics. For many organisms, main bottleneck sequence long reads remains lack robust methods obtain high-molecular-weight (HMW) DNA. this...
Chloroplast ATP synthase contains subunits of plastid and nuclear genetic origin. To investigate the coordinated biogenesis this complex, we isolated novel mutants in green alga Chlamydomonas reinhardtii by screening for high light sensitivity. We report here characterization affecting two peripheral stalk b b', encoded respectively atpF ATPG genes, three independent which identify factor MDE1, required to stabilize chloroplast-encoded atpE mRNA. Whole-genome sequencing revealed a transposon...
Abstract Marine life is threatened by global warming and its indirect consequences, which include, among others, increased stratification leading to phosphorus (P) nitrogen depletion in the upper water column. Phosphorus plays a key role all biochemical systems; storage of metabolic energy, formation genetic material, subcellular compartmentalization. Our multi‐year study lipid biogeochemistry northern Adriatic (Mediterranean), becoming warmer nutrient‐poorer, particularly regarding P, has...
Abstract Telomeres and subtelomeres, the genomic regions located at chromosome extremities, are essential for genome stability in eukaryotes. In absence of canonical maintenance mechanism provided by telomerase, telomere shortening induces instability. The landscape ensuing rearrangements is not accessible short-read sequencing. Here, we leverage Oxford Nanopore Technologies long-read sequencing to survey extensive repertoire telomerase mutants model green microalga Chlamydomonas reinhardtii...
Telomeres and subtelomeres, the genomic regions located at chromosome extremities, are essential for genome stability in eukaryotes. In absence of canonical maintenance mechanism provided by telomerase, telomere shortening induces instability. The landscape ensuing rearrangements is not accessible short-read sequencing. Here, we leverage Oxford Nanopore Technologies long-read sequencing to survey extensive repertoire telomerase mutants model green microalga
ABSTRACT Photosynthetic organisms have developed sophisticated strategies to fine-tune light energy conversion meet the metabolic demand, thereby optimizing growth in fluctuating environments. Although mechanisms such as dissipation, photosynthetic control, or photosystem II (PSII) damage and repair been widely studied, little is known about regulation of protein synthesis capacity during acclimation. By screening a Chlamydomonas reinhardtii insertional mutant library using chlorophyll...
Abstract Recent advances in long-read sequencing technologies have enabled the complete assembly of eukaryotic genomes from telomere to by allowing repeated regions be fully sequenced and assembled, thus filling gaps left previous short-read methods. Furthermore, can also help characterizing structural variants, with applications fields genome evolution or cancer genomics. For many organisms, main bottleneck sequence long reads remains lack robust methods obtain high-molecular-weight (HMW)...
Abstract Characterizing the pace of mutation accumulation is crucial not only for understanding how populations adapt to their environment but also unraveling intricate dynamics between gradual mutational processes and more sudden burst-like events occurring during cancer development. We engineered genome Saccharomyces cerevisiae measure rates single double mutations, including point segmental duplications reciprocal translocations. found that development wild-type yeast colonies grown in...
Recent advances in long-read sequencing technologies have enabled the complete assembly of eukaryotic genomes from telomere to by allowing repeated regions be fully sequenced and assembled, thus filling gaps left previous short-read methods. Furthermore, can also help characterizing structural variants, with applications fields genome evolution or cancer genomics. For many organisms, main bottleneck is develop robust methods obtain high-molecular-weight (HMW) DNA for whole purposes. We...
Recent advances in long-read sequencing technologies have enabled the complete assembly of eukaryotic genomes from telomere to by allowing repeated regions be fully sequenced and assembled, thus filling gaps left previous short-read methods. Furthermore, can also help characterizing structural variants, with applications fields genome evolution or cancer genomics. For many organisms, main bottleneck is develop robust methods obtain high-molecular-weight (HMW) DNA for whole purposes. We...
Recent advances in long-read sequencing technologies have enabled the complete assembly of eukaryotic genomes from telomere to by allowing repeated regions be fully sequenced and assembled, thus filling gaps left previous short-read methods. Furthermore, can also help characterizing structural variants, with applications fields genome evolution or cancer genomics. For many organisms, main bottleneck is develop robust methods obtain high-molecular-weight (HMW) DNA for whole purposes. We...
Abstract In most eukaryotes, subtelomeres are dynamic genomic regions populated by multi-copy sequences of different origins, which can promote segmental duplications and chromosomal rearrangements. However, their repetitive nature has complicated the efforts to sequence them, analyze structure infer how they evolved. Here, we use recent forthcoming genome assemblies Chlamydomonas reinhardtii based on long-read sequencing comprehensively describe subtelomere architecture 17 chromosomes this...