A5007405976- Osteoarthritis Treatment and Mechanisms
- Knee injuries and reconstruction techniques
- 3D Printing in Biomedical Research
- Tissue Engineering and Regenerative Medicine
- Nanoplatforms for cancer theranostics
- Tendon Structure and Treatment
- Protein purification and stability
- Silk-based biomaterials and applications
- Advancements in Transdermal Drug Delivery
- Optical Imaging and Spectroscopy Techniques
- Cancer, Hypoxia, and Metabolism
- Bone Tissue Engineering Materials
- Pluripotent Stem Cells Research
- Advanced Biosensing Techniques and Applications
- Medical Imaging and Pathology Studies
- Field-Flow Fractionation Techniques
- Microfluidic and Bio-sensing Technologies
- Medical Imaging Techniques and Applications
- Protein Interaction Studies and Fluorescence Analysis
- Mesenchymal stem cell research
- Hydrogels: synthesis, properties, applications
- Heterotopic Ossification and Related Conditions
- Nanofabrication and Lithography Techniques
- Connective tissue disorders research
- Cellular Mechanics and Interactions
University of Freiburg
2012-2019
Hospital Base
2012
University Hospital of Basel
2007-2009
Abstract Objective To determine whether engineered cartilage generated by nasal chondrocytes (ECN) is responsive to different regimens of loading associated with joint kinematics and previously shown be stimulatory articular (ECA). Methods Human chondrocytes, harvested from 5 individuals, were expanded cultured for 2 weeks into porous polymeric scaffolds. The resulting ECN ECA then maintained under static conditions or exposed the following regimens: regimen 1, single application cyclic...
Abstract Repeated passaging in conventional cell culture reduces pluripotency and proliferation capacity of human mesenchymal stem cells (MSC). We introduce an innovative method whereby the surface is dynamically enlarged during proliferation. This approach maintains constantly high density while preventing contact inhibition growth. A highly elastic was steps 5% over course a 20-day period to 800% initial area. Nine weeks dynamic expansion produced 10-fold more MSC compared with culture,...
Non-invasive in vivo imaging is emerging as an important tool for basic and preclinical research. Near-infrared (NIR) fluorescence dyes probes have been used non-invasive optical since the NIR region absorption auto by body tissue low, thus permitting greater penetration depths high signal to noise ratio. Currently, cell tracking systems rely on labeling cells prior injection or administering targeting population of choice right before imaging. These approaches do not enable tumor growth,...
This study aimed at testing whether an RGD-restricted substrate interface is sufficient for adhesion and growth of human articular chondrocytes (HAC), it enhances their post expansion chondrogenic capacity. HAC/substrate interaction was restricted to RGD by modifying tissue culture polystyrene (TCPS) with a poly(ethylene glycol) (PEG) based copolymer system that renders the surface resistant protein adsorption while same time presenting bioactive RGD-containing peptide GCRGYGRGDSPG (RGD). As...
The provision of mechanical stimulation is believed to be necessary for the functional assembly skeletal tissues, which are normally exposed a variety biomechanical signals in vivo. In this paper, we present development and validation novel bioreactor aimed tissue engineering that provides dynamic compression perfusion cultivated tissues. Dynamic can applied at frequencies up 67.5 Hz displacements down 5 ?m thus suitable simulation physiological conditions native cartilage (0.1-1 Hz, 5-10 %...
Stratum corneum, the outermost layer of skin, allows transport only low-molecular weight (<500) lipophilic solutes. Here, we report a surprising finding that avicins ( Av s), family naturally occurring glycosylated triterpenes with molecular > 2,000, exhibit skin permeabilities comparable to those small hydrophobic molecules, such as estradiol. Systematic fragmentation molecule shows deletion outer monoterpene results in 62% reduction permeability, suggesting an important role for this...
Controlled release drug delivery systems are well established as oral and implantable dosage forms. However, the controlled paradigm can also be used to present complex soluble signals responsible for cellular organization during development. Endochondral ossification (EO), developmental process of bone formation from a cartilage matrix is by several with distinct functions that vary in structure, molecular weight stability. This makes delivering them single vehicle rather challenging....
Soluble signal gradients play an important role in organ patterning, cell migration, and differentiation. Currently, 2D culture are realized using microfluidics here cells exposed to high nonphysiological shear stress. Tissue morphogenesis (organogenesis) however occurs 3D therefore there is a need for simple practical systems impose dispersed matrix. Herein, gradient generator based on passive diffusion elements that recapitulates interstitial flow capable of imposing predictable over long...
Systems for efficient and selective capture of micro-scale objects structures have application in many areas are particular relevance isolation mammalian cells. the latter should also not interfere with biology This study demonstrates microspheres through orthogonal coupling using biotin (ligand) (strept)avidin (receptor). Fibrous poly(ethylene terephthalate) (PET) meshes were hydrolyzed under controlled alkaline conditions to obtain activated surfaces COOH groups allowing functionalization...