A5045115719- Zebrafish Biomedical Research Applications
- Acute Myeloid Leukemia Research
- CRISPR and Genetic Engineering
- Epigenetics and DNA Methylation
- Immune Cell Function and Interaction
- RNA and protein synthesis mechanisms
- Innovation and Socioeconomic Development
- Platelet Disorders and Treatments
- Aquaculture disease management and microbiota
- Lipid metabolism and biosynthesis
- Neonatal Respiratory Health Research
- Insect symbiosis and bacterial influences
- Mast cells and histamine
- Advanced biosensing and bioanalysis techniques
- T-cell and B-cell Immunology
- Mesenchymal stem cell research
- Plant Virus Research Studies
- Acute Lymphoblastic Leukemia research
- Renal and related cancers
- Birth, Development, and Health
- Chronic Myeloid Leukemia Treatments
- Adipokines, Inflammation, and Metabolic Diseases
- Immunodeficiency and Autoimmune Disorders
- Immune responses and vaccinations
- Histone Deacetylase Inhibitors Research
Sunnybrook Health Science Centre
2021-2024
Sunnybrook Research Institute
2021-2024
University of Toronto
2023
Dalhousie University
2015-2022
University of Washington
2020
Izaak Walton Killam Health Centre
2015
Genome engineering has been revolutionized by the discovery of clustered regularly interspaced palindromic repeats (CRISPR) and CRISPR-associated system genes (Cas) in bacteria. The type IIB Streptococcus pyogenes CRISPR/Cas9 functions many species additional types CRISPR/Cas systems are under development. In II system, expression CRISPR single guide RNA (sgRNA) targeting a defined sequence Cas9 generates sequence-specific nuclease inducing small deletions or insertions. Moreover, knock-in...
Xenograft models are invaluable tools in establishing the current paradigms of hematopoiesis and leukemogenesis. The zebrafish has emerged as a robust alternative xenograft model but, like mice, lack specific cytokines that mimic microenvironment found human patients. To address this critical gap, we generated first humanized express hematopoietic-specific (GM-CSF, SCF, SDF1α). Termed GSS fish, these promote survival, self-renewal multilineage differentiation hematopoietic stem progenitor...
The development of clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 technology for mainstream biotechnological use based on its discovery as an adaptive immune mechanism in bacteria has dramatically improved the ability molecular biologists to modify genomes model organisms. zebrafish is highly amenable applications CRISPR/Cas9 mutation generation and a variety DNA insertions. Cas9 protein complex with guide RNA molecule recognizes where cut homologous stretch termed...
Background: Knock-in of precise point mutations into protein-coding genes is among the most important applications Clustered Regularly Interspaced Palindromic Repeats (CRISPR)/Cas9. Precise introduction amino acid changes crucial to interrogate function specific protein residues and create human disease models. Mutations homologous in model animal species enable studies their consequences, leading a better understanding question. Objectives: Manual design mutation knock-ins time-consuming...
Abstract KIT, a type III tyrosine kinase receptor, plays crucial role in haematopoietic development. The KIT receptor forms dimer after ligand binding; this activates activity leading to downstream signal transduction. D816V mutation is extensively implicated haematological malignancies, including mastocytosis and leukaemia. constitutively active, but the molecular nuances that lead constitutive are unclear. For first time, we present experimental evidence mutant does not dimerize like wild...
Abstract Two major developmental steps are necessary for the generation of innate IL-17 producing γδ T cells. First, cell precursors in fetal thymus commit to either αβ or lineage. This is followed by a second stage differentiation into subsets that can produce (γδT17) IFNγ (γδT1) Both events dependent on TCR signal strength, but how this interpreted at molecular level has been unclear. Id3, which antagonizes activity HEB transcription factors, upregulated proportion providing an important...
Abstract Knock-in of precise point mutations into protein-coding genes has been one the earliest and most important applications Clustered Regularly Interspaced Palindromic Repeats (CRISPR)/Cas9. The ability to perform such gene editing is crucial interrogate function specific protein residues create models human diseases caused by amino acid changes. homologous can be mutated in model animal species, consequences these studied, leading a better understanding disease question. Design...