Waveguide chip-based microscopy reduces the complexity of total internal reflection fluorescence (TIRF) microscopy, and adds features like large field view illumination, decoupling illumination collection path easy multimodal imaging. However, for technique to become widespread there is a need low-loss affordable waveguides made high-refractive index material. Here, we develop report silicon nitride (Si3N4) waveguide platform multi-color TIRF microscopy. Single mode conditions at visible...

Optical nanoscopy techniques can image intracellular structures with high specificity at sub-diffraction limited resolution, bridging the resolution gap between optical microscopy and electron microscopy. So far conventional lacks ability to generate throughput data, as imaged region is small. Photonic chip-based has demonstrated potential for imaging large areas, but a lateral of 130 nm. However, all existing super-resolution methods provide 100 nm or better. In this work, 75 over an...

Optical waveguides can be used to trap and transport micro-particles. The particles are held close the waveguide surface by evanescent field propelled forward. We propose a new technique lift above of waveguides. This is made possible gap between two opposing, planar emitted from each ends diverge fast, away substrate into cover-medium. By combining fields propagating at an angle upwards coming opposite sides gap, stably lifted trapped crossing fields. Thus, transported leading where they...

Photonic-chip based TIRF illumination has been used to demonstrate several on-chip optical nanoscopy methods. The sample is illuminated by the evanescent field generated electromagnetic wave modes guided inside waveguide. In addition photokinetics of fluorophores, waveguide can be further exploited for introducing controlled intensity fluctuations exploitation techniques such as super-resolution fluctuation imaging (SOFI). However, problem non-uniform pattern contribute artifacts in...

Abstract Super-resolution microscopy allows for stunning images with a resolution well beyond the optical diffraction limit, but imaging techniques are demanding in terms of instrumentation and software. Using scientific-grade cameras, solid-state lasers top-shelf objective lenses drives price complexity system, limiting its use to well-funded institutions. However, by harnessing recent developments CMOS image sensor technology low-cost illumination strategies, super-resolution can be made...

Rib waveguides are investigated as an alternative to strip for planar trapping and transport of microparticles. Microparticles successfully propelled along the surface rib trapped in gap between opposing waveguides. The capabilities waveguide end facets formed by a single geometries investigated. slab beneath continues guide light after facet waveguide. Thus particles can be wider gaps than with were found more efficient collection could moved different locations changing relative power two...

Abstract Correlative light and electron microscopy (CLEM) unifies the versatility of (LM) with high resolution (EM), allowing one to zoom into complex organization cells. Here, we introduce photonic chip assisted CLEM, enabling multi-modal total internal reflection fluorescence (TIRF) over large field view precision localization target area interest within EM. The chips are used as a substrate hold, illuminate provide landmarking sample through specially designed grid-like numbering systems....

On-chip super-resolution optical microscopy is an emerging field relying on waveguide excitation with visible light. Here, we investigate two commonly used high-refractive index platforms, tantalum pentoxide (Ta$_2$O$_5$) and silicon nitride (Si$_3$N$_4$), respect to their background in the range 488-640 nm. The strength from these waveguides were estimated by imaging fluorescent beads. spectral dependence of platforms was also measured. For 640 nm wavelength both materials had a weak...

Total internal reflection fluorescence (TIRF) is commonly used in single molecule localization based super-resolution microscopy as it gives enhanced contrast due to optical sectioning. The conventional approach use high numerical aperture microscope TIRF objectives for both excitation and collection, severely limiting the field of view throughput. We present a novel generating imaging with waveguides, called chip-based nanoscopy. aim this protocol demonstrate how performed an already built...

Super-resolution optical microscopy, commonly referred to as nanoscopy, has enabled imaging of biological samples with a resolution that was only achievable previously using electron microscopy. Optical nanoscopy is rapidly growing field, several different techniques and implementations overcome the diffraction limit light. However, common nanoscope continues be rather complex, expensive bulky instrument. Direct stochastic reconstruction microscopy (dSTORM) recently demonstrated waveguide...

Photonic chips have the potential to cause a paradigm shift in super-resolution optical microscopy. Here we discuss geometry optimization of high refractive index waveguides for bioimaging and implementation methods.

The evanescent field on top of optical waveguides is used to image membrane network and sieve-plates liver endothelial cells. In waveguide excitation, the dominant only near surface (~100-150 nm) providing a default sectioning by illuminating fluorophores in close proximity thus benefiting higher signal-to-noise ratio. sieve plates sinusoidal cells are present cell membrane, near-field chip-based microscopy configuration preferred over epi-fluorescence. chip compatible with fiber components...

Total internal reflection fluorescence (TIRF) is commonly used in single molecule localization based super-resolution microscopy as it gives enhanced contrast due to optical sectioning. The conventional approach use high numerical aperture microscope TIRF objectives for both excitation and collection, severely limiting the field of view throughput. We present a novel generating imaging with waveguides, called chip-based nanoscopy. aim this protocol demonstrate how performed an already built...

The evanescent field from an optical waveguide is used for near-field trapping and transporting of fluorescent microspheres. Out-of-focus fluorescence imaging to track the trapped particle in 3-D with nanometer precision (<100 nm). A prior calibration done determine relationship between z-coordinate radius outermost diffraction ring image sphere. This gives precise information about how much moves up down during propulsion along waveguide. Results are presented tracking a 1 μm on strip

Get PDF Email Share with Facebook Tweet This Post on reddit LinkedIn Add to CiteULike Mendeley BibSonomy Citation Copy Text Ø. I. Helle, D. A. Choucheron, C. Øie, J. Tinguely, and B. S. Ahluwalia, "On-Chip Nanoscopy," in Latin America Optics Photonics Conference, OSA Technical Digest (Optica Publishing Group, 2018), paper Th2A.4. Export BibTex Endnote (RIS) HTML Plain alert Save article

Correlative light-electron microscopy (CLEM) unifies the versatility of light (LM) with high resolution electron (EM), allowing one to zoom into complex organization cells. Most CLEM techniques use ultrathin sections, and thus lack 3D-EM structural information, focusing on a very restricted field view. Here, we introduce photonic chip assisted CLEM, enabling multi-modal total internal reflection fluorescence (TIRF) over large view precision localization target area interest within EM. The...

A photonic chip-based approach is shown as alternative to conventional super-resolution fluorescence microscopy. By making use of circuits, more compact, cost-effective and higher resolving setups can allow for a wider implementation these techniques.