A5027342673- Protein Tyrosine Phosphatases
- Galectins and Cancer Biology
- Cancer, Hypoxia, and Metabolism
- Estrogen and related hormone effects
- ATP Synthase and ATPases Research
- Heat shock proteins research
- Phytochemicals and Antioxidant Activities
- Bioactive Compounds and Antitumor Agents
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Free Radicals and Antioxidants
- Electron Spin Resonance Studies
- Mitochondrial Function and Pathology
- Nitric Oxide and Endothelin Effects
- Tannin, Tannase and Anticancer Activities
- Eicosanoids and Hypertension Pharmacology
- Glycosylation and Glycoproteins Research
- Synthesis and biological activity
- Bacteriophages and microbial interactions
- Yersinia bacterium, plague, ectoparasites research
- Antioxidant Activity and Oxidative Stress
- Vanadium and Halogenation Chemistry
- Redox biology and oxidative stress
- Tea Polyphenols and Effects
- Edible Oils Quality and Analysis
- Curcumin's Biomedical Applications
Gdańsk Medical University
2016-2025
Osteosarcoma is one of the most malignant tumors childhood and adolescence that often resistant to standard chemo- radio-therapy. Geldanamycin geldanamycin analogs have been recently studied as potential anticancer agents for osteosarcoma treatment. Here, first time, we presented novel mechanisms biological activity. Moreover, demonstrated an association between effects on major heat shock proteins (HSPs) overall survival highly metastatic human 143B cells. We treatment cells with caused a...
Background/Aim: Protein tyrosine phosphatase (PTP1B) is a potential target for the treatment of type 2 diabetes and cancer. Curcumin cinnamaldehyde have been previously reported to antidiabetic anticancer potentials. The aim this study was investigate effect curcumin in comparison on enzymatic activity PTP1B viability MCF-7 cancer cells. Materials Methods: Enzymatic cell assays were utilized. Experiments performed using breast line. Results: decreased PTP1B, had inhibitory effects...
The catechins derived from green tea possess antioxidant activity and may have a potentially anticancer effect. PTP1B is tyrosine phosphatase that oxidative stress regulated involved with prooncogenic pathways leading to the formation of a.o. breast cancer. Here, we present effect selected on enzymatic viability MCF-7 cancer cells. We showed also computational analysis most effective catechin binding molecule. observed epigallocatechin, epigallocatechin gallate, epicatechin, epicatechin...
Background/Aim: Oleuropein belongs to the potent polyphenols of olive oil. Notably, it is considered as a potentially active anticancer agent. Herein, efficiency oleuropein, when used separately and in combination with chemotherapeutic agent, 2-methoxyestradiol (2-ME), was investigated highly metastatic osteosarcoma (OS) cells. Materials Methods: Human OS cells (143B cell line) were incubated oleuropein 2-ME, alone or combination. Cell viability determined by MTT assay. migration assays...
This article describes the invention of graphene oxide (GO) or reduced (rGO) functionalised with 2-methoxy estradiol. The presence polar hydroxyl groups enables binding 2-ME to GO/rGO through hydrogen bonds epoxy and located on surface carbonyl carboxyl at edges flake sheets. patented method producing subject research results regarding its anticancer effectiveness via cytotoxicity in an vivo model (against A375 melanoma 143B osteosarcoma cells) are described. It was shown that inhibition...
Protein tyrosine phosphatases PTP1B and SHP2 are potential targets for anticancer therapy, because of the essential role they play in development tumors. overexpressed breast cancer cells, thus inhibition their activity can be potentially effective therapy. Lipoic acid has been previously reported to inhibit proliferation colon, thyroid cells.We investigated effect alpha-lipoic (ALA) its reduced form dihydrolipoic (DHLA) on viability MCF-7 cells enzymatic phosphatases.ALA DHLA decrease SHP2,...
A randomized prospective clinical study performed on a group of 74 pregnant women (43 presenting with severe preeclampsia) proved that urinary levels 15-F2t-isoprostane were significantly higher in preeclamptic patients relative to the control (3.05 vs. 2.00 ng/mg creatinine). Surprisingly enough, plasma 25-hydroxyvitamin D3 both groups below reference range no significant difference between groups. In vitro isolated placental mitochondria and cell line showed suicidal self-oxidation...
// Alicja Kuban-Jankowska 1 , Kamlesh K. Sahu 2 Magdalena Gorska Jack A. Tuszynski 2,3 and Michal Wozniak Department of Medical Chemistry, University Gdansk, Poland Physics, Alberta, Edmonton, Canada 3 Division Experimental Oncology, Cross Cancer Institute, Correspondence to: Kuban-Jankowska, email: Keywords : chicoric acid, caffeic chlorogenic protein tyrosine phosphatase YopH, Immunology Microbiology Section, Immune response, Immunity Received September 10, 2015 Accepted November 26,...
Background: Rapidly-dividing cancer cells have higher requirement for iron compared to non-transformed cells, making chelating a potential anticancer strategy. In the present study we activity of uncommon chelator aurintricarboxylic acid (ATA) with known deferoxamine (DFO). Materials and Methods: We investigated impact ATA DFO on viability proliferation MCF-7 cells. Moreover performed enzymatic assays computational analysis effects pro-oncogenic phosphatases PTP1B SHP2. Results: decrease...
Background/Aim: Dysregulation of mitochondrial pathways is implicated in several diseases, including cancer. Notably, respiration and biogenesis are favored some invasive cancer cells, such as osteosarcoma. Hence, the aim current work was to investigate effects 2-methoxyestradiol (2-ME), a potent anticancer agent, on osteosarcoma cells. Materials Methods: Highly metastatic 143B cells were treated with 2-ME separately or combination L-lactate, solvent (non-treated control cells). Protein...
2-Methoxyestradiol, one of the natural 17β-estradiol derivatives, is a novel, potent anticancer agent currently being evaluated in advanced phases clinical trials. The main goal study was to investigate activity 2-methoxy-estradiol towards osteosarcoma cells and its possible neurodegenerative effects. We used an experimental model neurotoxicity physiological agent, 2-methoxyestradiol. Thus, we highly metastatic 143B mouse immortalized hippocampal HT22 cell lines. were treated with...
// Magdalena Gorska 1 , Alicja Kuban-Jankowska Michal Zmijewski 2 Antonella Marino Gammazza 3, 4 Francesco Cappello Maciej Wnuk 5 Monika Gorzynik Iwona Rzeszutek Agnieszka Daca 6, 7 Anna Lewinska 8 Wozniak Department of Medical Chemistry, University Gdansk, Poland Histology, 3 Experimental Biomedicine and Clinical Neurosciences, Section Human Anatomy ''Emerico Luna'', Palermo, Italy Euro-Mediterranean Institute Science Technology, Genetics, Rzeszow, 6 Pathophysiology, Pathology Rheumatology,...
Breast cancer is the most common of women-it affects more than 2 million women worldwide. PTP1B phosphatase can be one possible targets for new drugs in breast therapy. In this paper, we present curcumin derivatives featuring a 4-piperidone ring as inhibitors and ROS inducers. We performed cytotoxicity analysis twelve against MCF-7 MDA-MB-231 cell lines human keratinocyte HaCaT line. Furthermore, because known antioxidant, assessed antioxidant effects its derivatives. For potent cytotoxic...
Deregulation of serine and glycine metabolism, have been identified to function as metabolic regulators in supporting tumor cell growth. The role regulation cancer proliferation is complicated, dependent on concentrations amino acids tissue-specific. D-serine are coagonists N-methyl-D-aspartate (NMDA) receptor subunit GRIN1. Importantly, NMDA receptors widely expressed cells play an important death, proliferation, metabolism numerous malignancies. aim the present work was associate with...
Background: The protein tyrosine phosphatases PTP1B and SHP2 are promising drug targets in treatment design for breast cancer. Searching specific inhibitors of their activity has recently become the challenge many studies. Previous work indicated that PTP may be small compounds able to bind interact with amino residues from binding site. Purpose: main goal our study was synthesize analyze effect selected peptide on oncogenic enzymatic viability MCF7 cancer cells. We also performed...
Lung cancer is one of the most common cancers worldwide, causing nearly million deaths each year. Herein, we present effect 2-methoxyestradiol (2-ME), endogenous metabolite 17β-estradiol (E2), on non-small cell lung (NSCLC) cells. We observed that 2-ME reduced viability adenocarcinoma in two-dimensional (2D) and three-dimensional (3D) spheroidal A549 culture models. Molecular modeling was carried out aiming to visualize amino acid residues within binding pockets acyl-protein thioesterases,...
Background: Docosahexaenoic acid (DHA) is an essential polyunsaturated fatty compound present in deep water fishes and dietary supplements, with a wide spectrum of potential health benefits, ranging from neurological to anti-inflammatory. Methods: Due the fact that DHA considered breast cancer risk reducer, we examined impact on MCF-7 cells’ viability its inhibitory properties protein tyrosine phosphatase 1B (PTP1B), pro-oncogenic phosphatase. Results: We found able lower both enzymatic...