A5058794130- Pineapple and bromelain studies
- Enzyme Catalysis and Immobilization
- Protein Hydrolysis and Bioactive Peptides
- Enzyme Production and Characterization
- Microbial Metabolites in Food Biotechnology
- Nanocomposite Films for Food Packaging
- Biochemical and Structural Characterization
- Advanced Drug Delivery Systems
- Proteins in Food Systems
- Electrochemical sensors and biosensors
- Phytochemistry and biological activities of Ficus species
- Synthesis and Characterization of Heterocyclic Compounds
- Peptidase Inhibition and Analysis
- Carbohydrate Chemistry and Synthesis
- Synthesis and Biological Evaluation
- Natural product bioactivities and synthesis
- Glycosylation and Glycoproteins Research
- Dye analysis and toxicity
- Click Chemistry and Applications
- Biopolymer Synthesis and Applications
- Pharmacological Effects of Natural Compounds
- Synthesis and biological activity
Voronezh State University
2017-2023
Chitosan, the product of chitin deacetylation, is an excellent candidate for enzyme immobilization purposes. Here we demonstrate that papain, endolytic cysteine protease (EC: 3.4.22.2) from Carica papaya latex immobilized on matrixes medium molecular (200 kDa) and high (350 weight chitosans exhibits anti-biofilm activity increases antimicrobials efficiency against biofilm-embedded bacteria. Immobilization in glycine buffer (pH 9.0) allowed adsorption up to 30% total protein (mg g chitosan−1)...
This work aims to synthesize graft copolymers of chitosan and N-vinylimidazole (VI) with different compositions be used as matrices for the immobilization cysteine proteases-bromelain, ficin, papain. The are synthesized by free radical solution copolymerization a potassium persulfate-sodium metabisulfite blend initiator. have relatively high frequency grafting yields. All water-soluble, their solutions characterized DLS laser Doppler microelectrophoresis. self-assembled in aqueous solutions,...
Briefly, 2-(4-Acetamido-2-sulfanilamide) chitosan, which is a chitosan water-soluble derivative, with molecular weights of 200, 350, and 600 kDa, was successfully synthesized. The immobilization ficin, papain, bromelain carried out by complexation these polymers. interaction mechanism 2-(4-acetamido-2-sulfanilamide) bromelain, papain studied using FTIR spectroscopy. It found that the hydroxy, thionyl, amino groups were involved in process. Molecular docking research showed all acid residues...
New substituted 4-aryl-8-methyl-2-thioxo-1,2,3,4-tetrahydro-6H-pyrimido[1,2-a][1,3,5]triazin-6-one 8a–b and ethyl 4-aryl-6-oxo-2-thioxo-1,3,4,6-tetrahydro-2H-pyrimido[1,2-a][1,3,5]triazine-7-carboxylate 8c–e were synthesized by the reaction of corresponding 4-oxopyrimidin-2-ylthioureas with arylaldehydes. The formation only one regioisomer was proven using complex spectral data its structure characterized. It found that interaction 6-amino-4-phenyl-3,4-dihydro-1,3,5-triazine-2(1H)-thione...
Plant enzymes such as ficin (EC 3.4.22.3), papain 3.4.22.2) and bromelain 3.4.22.4) are obtained from tropical plants. These biocatalysts belong to thiol proteases, in the active center of which cysteine is contained. Ficin, have a wide substrate specificity, provides demand for their use various industries. Enzymes free state less commonly used; immobilized preferred form. The aim this work was determine optimal concentration crosslinking agent covalent immobilization ficin, on chitosan...
Immobilized enzymes are the most sought-after preparations in global market. They used medicine, veterinary food industry, winemaking and brewing. The simplest method for immobilizing biocatalysts on insoluble carriers is simple adsorption method. Its advantage that it preserves natural conformation of enzyme, which slightly reduces its catalytic ability compared to native form. In our study, we carried out selection optimal conditions immobilization acid-soluble chitosan (Mr = 350 kDa)...
Трипсин (КФ 3.4.21.4) является наиболее популярным ферментом в промышленности и биомедицине. Однако невозможность повторного использования сложность восстановления делают его масштабное промышленное применение неэффективным дорогостоящим. Решить эту проблему можно с помощью иммобилизации трипсина на ионообменных материалах. Целью работы было исследование закономерностей адсорбционной смолах для создание гетерогенного препарата основе, доступного отечественных лабораториях...
Осуществлена ковалентная иммобилизация папаина на матрице кислоторастворимых среднемолекулярного и высокомолекулярного хитозанов, проведены сополимеризация в белковой пленке молекул фермента. Оптимальное соотношение содержания белка (мг г носителя), общей активности (в ед мл раствора) удельной мг белка) выявлено при ковалентной иммобилизации хитозана с 15 % глутаровым альдегидом 20 альдегидом.