A5036683091- Vector-borne infectious diseases
- Microbial infections and disease research
- Herpesvirus Infections and Treatments
- Viral Infections and Vectors
- Animal Virus Infections Studies
- Virus-based gene therapy research
- Rabies epidemiology and control
- Viral gastroenteritis research and epidemiology
- Virology and Viral Diseases
- Vector-Borne Animal Diseases
- Reproductive tract infections research
- Blood groups and transfusion
- Genetic Syndromes and Imprinting
- Genetic and Kidney Cyst Diseases
- Prion Diseases and Protein Misfolding
- Hepatitis B Virus Studies
- Mosquito-borne diseases and control
- Meat and Animal Product Quality
- Mycobacterium research and diagnosis
- Neurological diseases and metabolism
- Animal Disease Management and Epidemiology
- Veterinary Oncology Research
- Molecular Biology Techniques and Applications
- Inflammatory Bowel Disease
- Allergic Rhinitis and Sensitization
University of Bristol
2015-2025
The University of Sydney
2012
Institute of Infection and Immunity
2011
University of Liverpool
2011
Universitat Autònoma de Barcelona
2010
At Bristol
2005
Royal Agricultural University
2004
A full history of the management practices and prevalence upper respiratory tract disease (URTD) at 218 rescue shelters, breeding establishments private households with five or more cats was recorded. Oropharyngeal conjunctival swabs blood samples were taken from 1748 cats. The prevalences feline herpesvirus (FHV), calicivirus (FCV), Chlamydophila felis Bordetella bronchiseptica determined by PCR on swab samples. An ELISA applied to determine antibodies B. bronchiseptica. rates detection...
Recent changes in the distribution of tick vectors and incidence tick-borne disease, driven variously by factors such as climate change, habitat modification, increasing host abundance increased movement people animals, highlight importance ongoing, active surveillance. This paper documents results a large-scale survey on dogs presented to veterinary practices UK, using participatory approach that allows relatively cost- time-effective extensive data collection. Over period 16 weeks...
Recent evidence suggests that a mutation in the spike protein gene of feline coronavirus (FCoV), which results an amino acid change from methionine to leucine at position 1058, may be associated with infectious peritonitis (FIP). Tissue and faecal samples collected post mortem cats diagnosed or without FIP were subjected RNA extraction quantitative reverse-transcriptase polymerase chain reaction (qRT-PCR) detect FCoV RNA. In FIP, 95% tissue, 81% PCR-positive, as opposed 22% 60% FIP. Relative...
Feline infectious agent studies are lacking in Cyprus. The aims of this study were to determine the prevalence and risk factors for various feline agents, including vector-borne pathogens (FVBP), cats from A cross-sectional, descriptive, multicentre was performed on 174 samples [138 owned 36 shelter-feral, both healthy (43) non-healthy (131), cats] private veterinary clinics all six districts Real-time quantitative polymerase chain reaction (qPCR) assays used detect Mycoplasma haemofelis...
Fleas (Siphonaptera) are the most clinically important ectoparasites of dogs and cats worldwide. Rising levels pet ownership, climate change globalisation increasing importance a detailed understanding endemicity prevalence flea-borne pathogens. This requires continued surveillance to detect change. study reports large-scale survey pathogens in fleas collected from client-owned UK. Recruited veterinary practices were asked follow standardised flea inspection protocol on randomised selection...
Blood samples from 426 healthy and sick cats in the UK were tested a PCR assay for ‘Candidatus Mycoplasma haemominutum’ haemofelis (basonym Haemobartonella felis ). Seventy‐two of (16·9 per cent) positive M alone, six (1·4 alone one (0·2 was both. Logistic regression analysis indicated that older male significantly more likely to be infected with haemominutum’, but there no significant association between it any haematological variables measured. infection uncommon anaemic sampled, too few...
The aims of the present study were to determine prevalence hemoplasmas in cats and dogs from Barcelona area Spain with use species-specific quantitative polymerase chain reaction (qPCR) assays evaluate any associations between hemoplasma infection, clinical presentation, vector-borne infections. Blood samples (191) (182) included classified as healthy (149) or unhealthy (224). Ethylenediamine tetra-acetic acid blood underwent DNA extraction qPCR analysis. Mycoplasma haemofelis, ‘ Candidatus...
Two canine haemoplasma species have been recognised to date; Mycoplasma haemocanis (Mhc), which has associated with anaemia in splenectomised or immunocompromised dogs, and "Candidatus haematoparvum" (CMhp), recently described an anaemic dog undergoing chemotherapy. The study aim was develop quantitative real-time PCR assays (qPCRs) incorporating endogenous internal control detect Mhc CMhp apply these DNA samples extracted from blood collected Northern Tanzania (n = 100) dogs presented a...
The aim of this study was to compare blood copy, haematological and glucose values between cats experimentally infected with either Mycoplasma haemofelis (Group HF: 10 cats), 'Candidatus M. haemominutum' HM: 3 cats) or turicensis' TU: cats). Blood samples were collected regularly up 85 days post-infection (DPI) for haemoplasma real-time quantitative PCR, haematology, Coombs' testing measurement. Statistical analysis performed using a general linear model (ANOVA) appropriate repeated measures...
Feline infectious peritonitis (FIP) is a fatal disease of cats, and sequela systemic feline coronavirus (FCoV) infection. Mutations in the viral spike (S) gene have been associated with FCoVs found tissues from cats FIP, but not faeces healthy are implicated monocyte/macrophage tropism spread. This study was designed to determine whether S mutation analysis can reliably diagnose FIP. Cats were categorised as FIP (n = 57) or without 45) based on gross post-mortem histopathological examination...
Feline coronavirus (FCoV) infections are endemic among cats worldwide. The majority of asymptomatic or result in only mild enteric disease. However, approximately 5 % cases develop feline infectious peritonitis (FIP), a systemic disease that is frequent cause death young cats. In this study, we report the complete coding genome sequences six FCoVs: three from faecal samples healthy and tissue lesion with confirmed FIP. were obtained over period 8 weeks at single-site cat rescue rehoming...
The reduced cost and improved efficiency of whole genome sequencing (WGS) is drastically improving the development cats as biomedical models. Persian are models for Leber's congenital amaurosis (LCA), most severe earliest onset form visual impairment in humans. Cats with innocuous breed-defining traits, such a bobbed tail, can also be somite segmentation vertebral column development.
Abstract The development of high throughput SNP genotyping technologies has improved the genetic dissection simple and complex traits in many species including cats. properties feline 62,897 SNPs Illumina Infinium iSelect DNA array are described using a dataset over 2,000 samples, most extensive to date, representing 41 cat breeds, random bred population, four wild felid species. Accuracy efficiency array’s genotypes its utility performing population-based analyses were evaluated. Average...
Ticks derived from cats have rarely been evaluated for the presence of pathogens. The aim this study was to determine prevalence Anaplasma phagocytophilum, Bartonella spp., haemoplasma species and Hepatozoon spp. in ticks collected UK. Five hundred forty DNA samples extracted 540 presenting veterinarians UK practices were used. Samples underwent a conventional generic PCR assay detection real-time quantitative assays phagocytophilum three feline qPCR Feline 28S rDNA served as an endogenous...
ABSTRACT A real-time PCR assay using Taqman probes was developed to detect and quantify Mycoplasma haemofelis “ Candidatus haemominutum” in feline blood samples. The rapid sensitive successfully used monitor the vivo kinetics of cats experimentally infected with each species.
ABSTRACT Nearly complete 16S rRNA gene sequences for feline and canine hemoplasma isolates from Europe, Australia, Africa, Asia showed almost 100% identity to those previously reported United States isolates. Partial of the RNA subunit RNase P were also determined, P-based phylogenetic analysis that hemoplasmas are most closely related members Mycoplasma pneumoniae group.
Feline haemoplasma infection can cause haemolytic anaemia. The natural method of transmission haemoplasmas between cats is currently unknown but the nature some risk factors for suggests that saliva may act as a mode transmission. aim this study was to determine if Mycoplasma haemofelis (Mhf) and 'Candidatus haemominutum' (CMhm) DNAs could be amplified from salivary gland samples collected haemoplasma-infected cats.
Burmese is an old and popular cat breed, however, several health concerns, such as hypokalemia a craniofacial defect, are prevalent, endangering the general of breed. Hypokalemia, subnormal serum potassium ion concentration ([K+]), most often occurs secondary problem but can occur primary problem, hypokalaemic periodic paralysis in humans, feline polymyopathy primarily Burmese. The characteristic clinical sign skeletal muscle weakness that frequently episodic nature, either generalized, or...
The aim of this study was to develop quantitative real-time (q)PCR assays detect all known haemoplasma species, and a human housekeeping gene in order demonstrate both successful DNA extraction from clinical samples test for sample inhibition, apply these qPCRs blood smears. Sensitive specific generic qPCR were developed amplify as well glyceraldehyde-3-phosphate dehydrogenase (GAPDH) an internal amplification control. An optimized technique extracting stained smears also developed. These...