A5016753502- Enzyme function and inhibition
- Cholinesterase and Neurodegenerative Diseases
- Phenothiazines and Benzothiazines Synthesis and Activities
- Synthesis and Catalytic Reactions
- Polyamine Metabolism and Applications
- Chemical Reactions and Mechanisms
- Enzyme Catalysis and Immobilization
- Dielectric materials and actuators
- Advanced Sensor and Energy Harvesting Materials
- Electrochemical sensors and biosensors
- Ferroelectric and Piezoelectric Materials
- Cardiovascular Function and Risk Factors
- Adenosine and Purinergic Signaling
- Phytoplasmas and Hemiptera pathogens
- Cocoa and Sweet Potato Agronomy
- Biochemical and Molecular Research
- Receptor Mechanisms and Signaling
- Selenium in Biological Systems
- DNA Repair Mechanisms
- Histone Deacetylase Inhibitors Research
- Sulfur Compounds in Biology
- Metal complexes synthesis and properties
- Plant Pathogenic Bacteria Studies
- Cardiac electrophysiology and arrhythmias
- Cardiovascular and exercise physiology
Institute of Biosciences and Bioresources
2013-2025
National Research Council
2013-2024
Istituto per il Sistema Produzione Animale in Ambiente Mediterraneo
2020-2022
Ospedale Civile di Venezia
2022
Digital Proteomics (United States)
2020
University of Catania
2012-2016
Institute of Protein Biochemistry
2007-2016
University of Florence
2013-2015
Barcelona Biomedical Research Park
2015
Institut d'Investigacions Biomèdiques de Barcelona
2015
The biomimetic approach represents an interesting strategy for carbon dioxide (CO2) capture, offering advantages over other methods, due to its specificity CO2 and eco-compatibility, as it allows concentration of from gases, conversion water soluble ions. This uses microorganisms capable fixing through metabolic pathways or via the use enzyme, such carbonic anhydrase (CA, EC 4.2.1.1). Recently, our group cloned purified a novel bacterial α-CA, named SspCA, thermophilic bacteria,...
A new carbonic anhydrase (CA, EC 4.2.1.1) from the thermophilic bacterium Sulfurihydrogenibium yellowstonense YO3AOP1 was identified and characterized. The bacterial gene expressed in Escherichia coli yielding an active enzyme, which purified large amounts. recombinant protein (SspCA) found to belong α-CA class displays esterase activity. kinetic parameters were determined by using CO(2) p-nitrophenylacetate (p-NpA) as substrates. enzyme presented specific activity comparable that of bovine...
SspCA, a novel `extremo-α-carbonic anhydrase' isolated from the thermophilic bacterium Sulfurihydrogenibium yellowstonense YO3AOP1, is an efficient catalyst for hydration of CO2 and presents exceptional thermostability. Indeed, SspCA retains high catalytic activity even after being heated to 343-373 K several hours. Here, crystallographic structure this α-carbonic anhydrase (α-CA) reported factors responsible its function at temperature are elucidated. In particular, study suggests that...
Glaucoma, a leading cause of irreversible vision loss worldwide, is characterized by elevated intraocular pressure (IOP), well-established risk factor across all its forms. We present the design and synthesis 39 novel carbonic anhydrase inhibitors dual-tailed approach, strategically crafted to interact with distinct hydrophobic hydrophilic pockets CA active sites. The series was investigated against isoforms implicated in glaucoma (hCA II, hCA IV, XII), X-ray crystal structures compounds
We have cloned, purified, and characterized an α-carbonic anhydrase (CA, EC 4.2.1.1) from the human pathogenic bacterium Vibrio cholerae, VchCA. The new enzyme has significant catalytic activity, inhibition study with sulfonamides sulfamates led to detection of a large number low nanomolar inhibitors, among which are methazolamide, acetazolamide, ethoxzolamide, dorzolamide, brinzolamide, benzolamide, indisulam (KI values in range 0.69-8.1 nM). As bicarbonate is virulence factor this since...
Abstract Vibrio cholerae, a Gram-negative bacterium, is the causative agent of cholera and colonizes upper small intestine where sodium bicarbonate present at high concentration. Sodium potential inducer virulence gene expression. Bacteria can increase cytosolic levels through existence transporter family proteins or action metalloenzymes, called carbonic anhydrases (CAs, EC 4.2.1.1). lacking in its genome, utilizes CA system to accumulate into cell suggesting pivotal role this...
All proteolytic enzymes, which are able to renature and reacquire the activity on a copolymerized substrate, can be analyzed by zymography upon removal of sodium dodecyl sulfate (SDS). Protonography, new technique described in this study, unlike zymography, allows detection different protein, not protease, i.e. carbonic anhydrase (CA, EC 4.2.1.1) SDS polyacrylamide gel electrophoresis gel. CAs zinc-containing enzymes that catalyze reversible conversion carbon dioxide bicarbonate protons....
Carbonic anhydrases (CAs, EC 4.2.1.1) belonging to the α-, β-, γ-, δ- and ζ-CAs are ubiquitous metalloenzymes present in prokaryotes eukaryotes. CAs started be investigated detail only recently pathogenic bacteria, search for antibiotics with a novel mechanism of action, since it has been demonstrated that many such organisms they essential life cycle organism. CA inhibition leads growth impairment or defects several bacteria. The microbiota human oral mucosa consists myriad bacterial...
Diatom genome sequences clearly reveal the presence of different systems for HCO3(-) uptake. Carbon-concentrating mechanisms (CCM) based on transport and a plastid-localized carbonic anhydrase (CA, EC 4.2.1.1) appear to be more probable than others because CAs have been identified in many diatoms. are key enzymes involved acquisition inorganic carbon photosynthesis phytoplankton, as they catalyze efficiently interconversion between dioxide bicarbonate. Five genetically distinct classes...
The antimalarial drugs are of fundamental importance in the control malaria, especially for lack efficient treatments and acquired resistance to existing drugs. For this reason, there is a continuous work identifying novel, less toxic effective chemotherapies as well new therapeutic targets against causative agents malaria. In context, superfamily metalloenzymes named carbonic anhydrases (CAs, EC 4.2.1.1) has aroused great interest druggable enzymes limit development Plasmodium falciparum...
Helicobacter pylori, a Gram-negative neutrophilic pathogen, is the cause of chronic gastritis, peptic ulcers, and gastric cancer in humans. Current therapeutic regimens suffer from an emerging bacterial resistance rate poor patience compliance. To improve discovery compounds targeting alternative enzymes or essential pathways such as carbonic anhydrases (CAs), we assessed anti-H. pylori activity thymol carvacrol terms CA inhibition, isoform selectivity, growth impairment, biofilm production,...
Carbonic anhydrases (CAs, EC 4.2.1.1) catalyze a simple but physiologically relevant reaction in all life kingdoms, carbon dioxide hydration to bicarbonate and protons. CAs are present many pathogenic species involved the metabolism/biosynthetic reactions involving this ion. Ubiquity of these enzymes suggests pivotal role microbial virulence pathogenicity. Porphyromonas gingivalis is an anaerobic bacterium, which colonizes oral cavity, being pathogenesis periodontitis, inflammatory disease...