A5038382123- Epigenetics and DNA Methylation
- Genetic Syndromes and Imprinting
- Prenatal Screening and Diagnostics
- Genetics and Neurodevelopmental Disorders
- Immune Cell Function and Interaction
- Birth, Development, and Health
- RNA modifications and cancer
- Autoimmune and Inflammatory Disorders Research
- Artificial Intelligence in Healthcare
- Assisted Reproductive Technology and Twin Pregnancy
- Gestational Diabetes Research and Management
- Machine Learning in Healthcare
- Systemic Sclerosis and Related Diseases
- RNA Research and Splicing
- Identity, Memory, and Therapy
- Reproductive Biology and Fertility
- Reproductive Health and Technologies
- Autism Spectrum Disorder Research
- Genomics and Rare Diseases
- Pluripotent Stem Cells Research
- Nuclear Structure and Function
- DNA Repair Mechanisms
- Genomics and Chromatin Dynamics
- Tryptophan and brain disorders
- COVID-19 Impact on Reproduction
Hamad bin Khalifa University
2019-2025
Qatar Foundation
2021-2025
University of Würzburg
2010-2019
Institute of Human Genetics
2011-2016
American University of Beirut Medical Center
2007-2014
Johannes Gutenberg University Mainz
2008-2012
University Medical Center of the Johannes Gutenberg University Mainz
2012
Ludwig-Maximilians-Universität München
2010
Epigenetic processes are primary candidates when searching for mechanisms that can stably modulate gene expression and metabolic pathways according to early life conditions. To test the effects of gestational diabetes mellitus (GDM) on epigenome next generation, cord blood placenta tissue were obtained from 88 newborns mothers with dietetically treated GDM, 98 insulin-dependent 65 without GDM. Bisulfite pyrosequencing was used compare methylation levels seven imprinted genes involved in...
Healthcare data are inherently multimodal, including electronic health records (EHR), medical images, and multi-omics data. Combining these multimodal sources contributes to a better understanding of human provides optimal personalized healthcare. The most important question when using is how fuse them-a field growing interest among researchers. Advances in artificial intelligence (AI) technologies, particularly machine learning (ML), enable the fusion different modalities provide insights....
Stochastic, environmentally and/or genetically induced disturbances in the genome-wide epigenetic reprogramming processes during male germ-cell development may contribute to infertility. To test this hypothesis, we have studied methylation levels of 2 paternally (H19 and GTL2) 5 maternally methylated (LIT1, MEST, NESPAS, PEG3, SNRPN) imprinted genes, as well ALU LINE1 repetitive elements 141 sperm samples, which were used for assisted reproductive technologies (ART), including 106 couples...
Intrauterine exposure to gestational diabetes mellitus (GDM) confers a lifelong increased risk for metabolic and other complex disorders the offspring. GDM-induced epigenetic modifications modulating gene regulation persisting into later life are generally assumed mediate these elevated disease susceptibilities. To identify candidate genes fetal programming, we compared genome-wide methylation patterns of cord bloods (FCBs) from GDM control pregnancies. Using Illumina's 450K arrays following...
Does ICSI induce specific DNA methylation changes in the resulting offspring? Although several thousand analyzed CpG sites (throughout genome) displayed significant between-group differences, both and spontaneously conceived children varied within normal range of variation. Children by ART have increased risks for medical problems at birth to extent present knowledge also later life (i.e. impaired metabolic cardiovascular functions). One plausible mechanism mediating these effects are...
Abstract Diabetes mellitus (DM) is a prevalent chronic metabolic disorder linked to increased morbidity and mortality. With significant portion of cases remaining undiagnosed, particularly in the Middle East North Africa (MENA) region, more accurate accessible diagnostic methods are essential. Current tests like fasting plasma glucose (FPG), oral tolerance (OGTT), random (RPG), hemoglobin A1c (HbA1c) have limitations, leading misclassifications discomfort for patients. The aim this study...
DNA methylation is an epigenetic modification that plays important role in gene regulation. It can be influenced by stochastic events, environmental factors and developmental programs. However, little known about the natural variation of gene-specific patterns. In this study, we performed quantitative analyses six differentially methylated imprinted genes ( H19 , MEG3 LIT1 NESP55 PEG3 SNRPN ), one hypermethylated pluripotency OCT4 ) hypomethylated tumor suppressor APC chorionic villus, fetal...
Cryopreservation of follicles for culture and oocyte growth maturation in vitro provides an option to increase the number fertilizable oocytes restore fertility cases where transplantation ovarian tissue poses a risk malignant cell contamination. Vitrification cryopreservation is fast avoids ice crystal formation. However, influences exposure high concentrations cryoprotectants on follicle development, maturation, particularly, DNA integrity methylation imprinting has not been studied...
Using Illumina 450K arrays, 1.85% of all analyzed CpG sites were significantly hypermethylated and 0.31% hypomethylated in fetal Down syndrome (DS) cortex throughout the genome. The methylation changes on chromosome 21 appeared to be balanced between hypo- hyper-methylation, whereas, consistent with prior reports, other chromosomes showed 3-11 times more hyper- than hypo-methylated sites. Reduced NRSF/REST expression due upregulation DYRK1A (on 21q22.13) REST binding during early...
Children of older fathers carry an increased risk for developing autism and other disorders. To elucidate the underlying mechanisms, we investigated correlation sperm DNA methylation with paternal age its impact on epigenome offspring. Methylation levels nine candidate genes LINE-1 repeats were quantified by bisulfite pyrosequencing in 162 donors 191 cord blood samples resulting children (conceived IVF/ICSI same samples). Four showed a significant negative between age. For FOXK1 KCNA7,...
The epigenome is thought to mediate between genes and the environment, particularly in response adverse life experiences. Similar other psychiatric diseases, suicide liability of an individual appears be influenced by many genetic factors small effect size as well environmental stressors. To identify epigenetic marks associated with suicide, which considered endpoint complex gene-environment interactions, we compared cortex DNA methylation patterns 6 completers versus non-psychiatric...
To study possible effects of assisted reproductive technologies (ART) on epigenetic reprogramming, we have analyzed the DNA methylation levels differentially methylated regions (DMRs) seven imprinted genes (H19, MEG3, LIT1, MEST, NESP55, PEG3 and SNRPN) as well promoter pluripotency gene NANOG tumor suppressor APC in chorionic villus samples (CVS) 42 spontaneous miscarriages stillbirths after ART 29 abortions/stillbirths conception. We did not find an increased rate faulty patterns ART, but...
Abstract Recently, a genome-wide analysis identified DNA methylation of the HIF3A ( hypoxia-inducible factor 3A ) as strongest correlate BMI. Here we tested hypothesis that mRNA expression and CpG-sites in adipose tissue (AT) genetic variants are related to parameters AT distribution function. In paired samples subcutaneous (SAT) visceral (VAT) from 603 individuals, measured analyzed its correlation with obesity traits. subgroups investigated effects on (N = 603) 87). was significantly...
The prevalence of metabolic disorders, in particular obesity has dramatically increased worldwide. Genetic variants explain only a minor part the epidemic induced by physical inactivity and over-nutrition. Epidemiological studies humans animal models indicate that epigenetic changes associated with adverse parental and/or intrauterine factors may contribute to missing heritability disorders. Possible paternal effects are likely transmitted sperm next-generation. To investigate this...
Hutchinson-Gilford Progeria Syndrome (HGPS) is an extremely rare genetic disorder caused by mutations in the LMNA gene and characterized premature accelerated aging beginning childhood. In this study, we performed first genome-wide methylation analysis on blood DNA of 15 patients with progeroid laminopathies using Infinium Methylation EPIC arrays including 8 classical HGPS. We could observe alterations at 61 CpG sites as well 32 significant regions following a 5 Kb tiling analysis....