A5054840119- Trace Elements in Health
- DNA and Nucleic Acid Chemistry
- Metal complexes synthesis and properties
- Drug Transport and Resistance Mechanisms
- Biochemical and Structural Characterization
- RNA and protein synthesis mechanisms
- DNA Repair Mechanisms
- Mercury impact and mitigation studies
- Signaling Pathways in Disease
- Effects of Vibration on Health
- Regulation of Appetite and Obesity
- Molecular Sensors and Ion Detection
- Advanced biosensing and bioanalysis techniques
- Genomics and Chromatin Dynamics
- Bacterial Genetics and Biotechnology
- Photosynthetic Processes and Mechanisms
- Biochemical Analysis and Sensing Techniques
- Metalloenzymes and iron-sulfur proteins
- Enzyme Structure and Function
- Spectroscopy and Quantum Chemical Studies
- Plant Stress Responses and Tolerance
- Structural Engineering and Vibration Analysis
- Heavy Metal Exposure and Toxicity
- Protein Structure and Dynamics
- Mechanical Engineering and Vibrations Research
University of Wrocław
2017-2022
Phytochelatins (PCs) are short Cys-rich peptides with repeating γ-Glu-Cys motifs found in plants, algae, certain fungi, and worms. Their biosynthesis has been to be induced by heavy metals—both biogenic toxic. Among all metal inducers, Cd(II) the most explored from a biological chemical point of view. Although Cd(II)-induced PC widely examined, still little is known about structure complexes their thermodynamic stability. Here, we systematically investigated glutathione (GSH) PC2–PC6...
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Nucleobindin-2 (Nucb2) is a protein that has been suggested to play roles in variety of biological processes. Nucb2 contains two Ca2+/Mg2+-binding EF-hand domains separated by an acidic amino acid residue-rich region and leucine zipper. All these are located within the C-terminal half protein. At N-terminal half, also possesses putative Zn2+-binding motif. In our recent studies, we observed underwent Ca2+-dependent compaction formed mosaic-like structure consisting intertwined disordered...
Abstract Nesfatin-1 and -2 are produced from a reaction in which the N-terminus of human Nucleobindin-2 undergoes proteolytical processing. To date, and/or nesfatin-1 have only been shown to act as peptide hormones. On other hand, purpose nesfatin-2 remains unknown. Since Nucleobindin-2/nesfatin-1 is thought impact control wide range physiological processes, including energy homeostasis, neurodegenerative processes carcinogenesis, its ligands/interactions deserve special studies attention....
Using a wheelchair over uneven terrain generates vibrations of the human body. These result from mechanical energy impulses transferred ground through components to user's body, which may negatively affect quality use and health. This can be dissipated structure frame, such as polymer carbon fiber composites. article aims compare with an aluminum alloy frame in terms reducing kinematic excitation acting on Three wheelchairs were used study, one (reference) two innovative ones composite...
In nature, thiolate-based systems are the primary targets of divalent mercury (HgII ) toxicity. The formation Hg(Cys)x cores in catalytic and structural protein centers mediates mercury's toxic effects ultimately leads to cellular damage. Multiple studies have revealed distinct HgII -thiolate coordination preferences, among which linear complexes most commonly observed solution at physiological pH. Trigonal or tetrahedral geometries formed basic pH tight intraprotein Cys-rich metal sites. So...
The metal binding at protein-protein interfaces is still uncharted territory in intermolecular interactions. To date, only a few protein complexes Zn(II) an manner have been deeply investigated. most notable example of such located the highly conserved Rad50 protein, part Mre11-Rad50-Nbs1 (MRN) complex, where required for homodimerization (Zn(Rad50)2). high stability Zn(Rad50)2 not derived from thermophilic archaeon Pyrococcus furiosus (logK12 = 20.95 130-amino-acid-long fragment), which was...
An extreme affinity of CdII towards the interprotein Rad50 zinc hook domain visualizes how a ZnII-to-CdII swap can affect target protein's status and thus illuminate molecular basis CdII-induced genotoxicity. This work showed that binding to induces two-orders-of-magnitude stabilization effect, which disrupts overall assembly may cause dysfunction MRN/X complex. More information be found in Full Paper by A. Krężel, et al. page 3297.
Abstract Invited for the cover of this issue is group Artur Krężel at University Wrocław in collaboration with Lars Hemmingsen The Copenhagen and Eva Freisinger Zürich. image depicts outcomes Hg II interactions Rad50 protein. Read full text article 10.1002/chem.202202738 .