PURPOSE Eosinophils are frequently found in atopic dermatitis (AD) and chronic spontaneous urticaria (CSU) that release eosinophil peroxidase (EPX) cationic protein (ECP). Continuous exposure to these proteins could trigger an autoimmune response which may contribute the pathogenesis severity of skin inflammation. In this study, we investigate immunoglobulin E (IgE) against CSU AD. METHODS We recruited patients with severe AD, healthy subjects explore presence IgE autoantibodies...

The house dust mite (HDM) Dermatophagoides pteronyssinus is an important risk factor for asthma and rhinitis. Allergen specific immunotherapy that based on recombinant proteins has been proposed the safer more efficient treatment of allergic diseases. aim this study was to design obtain a hybrid protein (DPx4) containing antigenic regions allergens Der p 1, 2, 7, 10 from mite. DPx4 produced in Escherichia coli its folding determined by circular dichroism. Non-denaturing dot-blot, ELISA,...

Cross-reactivity between allergens and human proteins could have a clinical impact in allergic diseases. Blo t 13 is an allergen from the mite Blomia tropicalis, which belongs to fatty acid binding protein (FABP) family has structural homology with FABPs. This work aimed map B cell epitopes on identify involved cross-reactivity heart FABP (FABP3) adipocyte (FABP4). Sera 25 patients house dust (HDM) allergy that were sensitized used for testing reactivity of immunoglobulin E (IgE) IgG FABP....

The shrimp Litopenaeus vannamei is an important source of food allergens but its allergenic repertoire poorly characterized. Cross-reactivity between crustacean and mites has been reported, with tropomyosin, the most relevant allergen involved. aim this study was to investigate structural immunological properties a recombinant Fatty Acid Binding Protein (FABP) family from L. (LvFABP).ELISA, skin prick test (SPT) basophil activation assays were performed determine IgE reactivity activity...

IgE sensitization (atopy) to pets is commonly evaluated using pet dander extracts. However, the diagnosis by components seems be more adequate evaluate clinical relevance (allergy) of sIgE sensitization. To study association between allergen and symptoms. Methodology. Dander extracts levels (Can f 1, Can 2, 3, 5, Fel d 4) were measured in a rhinitis group (n = 101) control (. Dog (34.6% vs. 23.5%) cat (26.7% 8.8%, p 0.05) was frequent among no-rhinitis subjects, it similar dog (29.7% 20.5%)...

The characterization of active compounds in Cnidaria sheds light on a large bank substances against multiresistant bacteria related to diseases humans, which makes it cutting edge with repertoire antimicrobial molecules worthy bioprospecting analysis. Thus, the main nabof this research was characterize peptides (AMP) belonging defensin family different species Cnidarians through bioinformatic approaches. To this, an exhaustive search carried out for sequences homologous genomes availables...

Allergy to shellfish is one of the most prevalent food allergies in several countries, especially induced by consuming or having contact with shrimp. Several shrimp species are known induce allergy diseases. However, whole spectrum allergens they contain unknown and few them have been completely characterized.This study was done order know recent advances characterization its relationship from other arthropods importance allergic We emphasize Litopenaeus vannamei, consumed Colombia. Well...

Helicobacter pylori has been linked to several diseases such as chronic urticaria, gastritis, and type 1 gastric neuroendocrine tumors (type gNET). Although these seem have different mechanisms, their relationship with H. suggests a common inflammatory pathway.To identify potential cross-reactive antigens between humans involved in urticaria gNET.Alignment was carried out among human proteins associated (9 proteins), gNET (32 proteome. We performed pairwise alignment the PSI-BLAST. Modeling...

Lipocalins are one of the groups allergens derived from domestic animals with clinical importance for development allergic responses. They have been characterized in different animals. With allergenic capacity characterized, little is known about epitopes involved Here, potential antigenic regions cross-reactivity among lipocalins were explored through bioinformatics tools. The amino acid sequences several (mouse, dog, cat, bull, hamster, horse and pig) used to determine degree kinship by...

<ns3:p><ns3:bold>Background:</ns3:bold> Phospholipases are enzymes with the capacity to hydrolyze membrane lipids and have been characterized in several allergenic sources, such as hymenoptera species. However, cross-reactivity among phospholipases allergens little understood. The objective of this study was determine potential antigenic regions involved using an <ns3:italic>in silico</ns3:italic> approach.</ns3:p><ns3:p> <ns3:bold>Methods:</ns3:bold> In total, 18 amino acids sequences...

Background: Lipocalins play a role in the cellular trafficking of pheromones and are involved allergic responses to domestic pets. However, cross-reactivity among allergens this group has been poorly explored, pheromone linking capacity is not well characterized. The aim study was explore cross-reactive epitopes Rat n 1 homologues pets through an silico approach. Methods: ElliPro BepiPred tools were used predict B cell linear epitopes. explored by docking virtual screening with...

<ns3:p>Background Atopic dermatitis (AD) is a chronic inflammatory skin condition that has significant impact on quality of life. The immune response and allergy symptoms in AD are triggered by the recognition specific allergens IgE antibodies. Cross-reactivity can lead to auto-IgE responses, potentially worsening symptoms. Our research aimed enhance our understanding allergenic sources, including A. fumigatus, their role AD. We focused molecular mimicry between human AQP3 fumigatus...

Background: Atopic dermatitis (AD) profoundly impacts quality of life as a chronic inflammatory skin disease. Allergen exposure triggers specific IgE antibodies, initiating allergy symptoms. Cross-reactivity, linked to auto-IgE responses, potentially influences AD severity. Exploring new allergens and self-autoantigens, like Aspergillus fumigatus, enhances knowledge triggers. Our focus was on analyzing molecular mimicry between human AQP3 A. fumigatus aquaporin, alongside various allergenic...

&lt;b&gt;&lt;i&gt;Introduction:&lt;/i&gt;&lt;/b&gt; Multiple antigen environmental sources have been identified as possible causes of allergies, but few studies evaluated changes in the sensitization profiles over time. The aim this study was to evaluate IgE and exposure dust mites, cats, dogs, cockroaches a 10-year period. &lt;b&gt;&lt;i&gt;Methods:&lt;/i&gt;&lt;/b&gt; During period 10 years among patients with asthma, rhinitis and/or atopic dermatitis, we annual frequency atopy...

Atopic dermatitis (AD) is a chronic inflammatory skin condition that has significant impact on quality of life. The immune response and allergy symptoms in AD are triggered by the recognition specific allergens IgE antibodies. Cross-reactivity can lead to auto-IgE responses, potentially worsening symptoms. Our research aimed enhance our understanding allergenic sources, including A. fumigatus, their role AD. We focused molecular mimicry between human AQP3 fumigatus aquaporin.

Introducción: Der f 24, que es un alérgeno caracterizado de Dermatophagoides farinae, homólogo la proteína unión ubiquinol citocromo c reductasa (UQCRB). Datos experimentales revelaron reactividad IgE en 24 se concentra epítopo ubicado las posiciones aminoácidos 1-32, correspondiente a región N-terminal. Sin embargo, posible cruzada entre y otras fuentes alergénicas no ha sido explorada utilizando enfoques o in silico. Objetivo: este estudio, datos previamente publicados herramientas...