Nanoparticles (NPs) are conventionally produced by using physical and chemical methods that no longer in alignment with current society’s demand for a low environmental impact. Accordingly, green synthesis approaches considered potential alternative due to the plant extracts substitute some of hazardous reagents. The general mechanism is based on reducing power natural products allows formation NPs from precursor solution. In this context, our study proposes simple, innovative, reproducible...

Microplastics (plastic particles < 5 mm) are ubiquitous pollutants that have the ability to carry microbiota, including pathogens. Microbial adhesion is usually a sign of pathogenicity; thus, we investigated adherent microbiota found on 4 mm nylon strips, which were ingested and excreted by wild fish specimens. Retention times recorded polymer analysis samples was performed, showed no signs degradation, nor did their controls, represented strips submerged in same water tanks. Both...

Glycosylation is a critical post-translational modification that influences protein folding, stability and function. While extensively studied in extracellular intracellular regions, glycosylation within transmembrane (TM) regions at membrane interfaces remains poorly understood. This study aimed to map O- N-glycosylation sites these using comprehensive database search structural validation where possible. Extensive searches revealed range of proteins. Only the falling TM interface...

Cenobamate is a new and highly effective antiseizure compound used for the treatment of adults with focal onset seizures particularly epilepsy resistant to other antiepileptic drugs. It acts on multiple targets, as it positive allosteric activator γ-aminobutyric acid type A (GABAA) receptors an inhibitor neuronal sodium channels, late or persistent Na+ current. We recently evidenced inhibitory effects cenobamate peak current component human cardiac isoform hNav1.5. The determined apparent...

During an Antarctic expedition that took place in December 2010–January 2011 the East coastal region, soil samples were collected aseptic conditions and stored for over a decade freezers at −20 °C. Due to shortly afterward passing of researcher charge, Teodor Negoiță, remained unintentionally frozen long period made available research 13 years later. A chemical analysis as well screening viable microbial presence was performed; conducted via inductively coupled plasma atomic emission...

Abstract Due to their outstanding properties, quantum dots (QDs) received a growing interest in the biomedical field, but it is of major importance investigate and understand interaction with biomolecules. We examined stability silicon QDs time evolution – protein corona formation various biological media (bovine serum albumin, cell culture medium without or supplemented 10% fetal bovine serum-FBS). Changes secondary structure BSA were also investigated over time. Hydrodynamic size zeta...

The enhancement of seed germination by using nanoparticles (NPs) holds the potential to elicit synthesis more desired compounds with important biomedical applications, such as preventing protein glycation, which occurs in diabetes. Here, we used 7 nm and 100 ZnO 4.5 16.7 Fe

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection can result in severe damage to the system. With no specific treatment date, it is crucial identify potent inhibitors of SARS-CoV-2 Chymotrypsin-like protease (3CLpro) that could also modulate enzymes involved accompanies infection. Here, flavones isolated from Scutellaria baicalensis (baicalein, baicalin, wogonin, norwogonin, and oroxylin A) were studied as possible compounds SARS-CoV-2-induced lung injuries. We used...

Background: Alzheimer's disease (AD) therapy is based on several natural and synthetic compounds that act as acetylcholinesterase (AChE) N-methyl-D-aspartate receptor (NMDA) ligands have limited efficiency in relieving AD symptoms. Recent studies show inhibitors isolated from Mentha spicata L. subsp. are promising for therapy. Objective: We aimed to identify novel more potent phytopharmaceutical treatment by taking into account the essential oil. Method: generated structure-activity...

Two different types of nanoparticles (silicon dioxide and titanium dioxide) were selected within this study in order to analyze the interaction with bovine human serum albumin. These particles characterized by transmission scanning electron microscopy (TEM SEM), X-ray diffraction (XRD) energy dispersive spectroscopy (EDXS). In addition, hydrodynamic size zeta potential measured for all these nanoparticles. The proteins incubated up one hour, albumin adsorption on particle surface was...

The current treatment of depression involves antidepressant synthetic drugs that have a variety side effects. In searching for alternatives, natural compounds could represent solution, as many studies reported such modulate the nervous system and exhibit We used bioinformatics methods to predict effect ten with neuroleptic activity, in literature. For all we computed their drug-likeness, absorption, distribution, metabolism, excretion (ADME), toxicity profiles. Their activities were...

Background: Transient receptor potential channels (TRP) are overexpressed in some pancreatic adenocarcinoma (PDAC) patients and cell lines, settling them as putative therapeutic targets this disease. Reactive oxygen species (ROS), with levels increased PDAC, modulate members of the TRP family renamed “redox channels”. Here, we investigate direct effects 4-hydroxinonenal (4-HNE) on TRPA1, natively expressed PDAC lines association migration cycle progression. Methods: We performed...

The therapeutic effects of curcumin and its derivatives, based on research in recent years, are limited by their low bioavailability. To improve bioavailability develop the medical field application, different delivery systems have been developed that adapted to certain environments or proposed target type. This study presents some half-curcuminoids prepared condensation acetylacetone with 4-hydroxybenzaldehyde (C1), 4-hydroxy-3-methoxybenzaldehyde (C2), 4-acetamidobenzaldehyde (C3),...

THz spectroscopy is important for the study of ion channels because it directly addresses low frequency collective motions relevant their function. Here we used to investigate inhibition epithelial sodium channel (ENaC) by its specific blocker, amiloride. Experiments were performed on A6 cells' suspensions, which are cells overexpressing ENaC derived from Xenopus laevis kidney. spectra investigated with or without When was inhibited amiloride, a substantial increase in absorption noticed....

The central nervous system is endowed with complex mechanisms of defense. However, these protection fail in the presence risk factors such as genetic mutations, environmental (bisphenol A, polycyclic aromatic hydrocarbons (PAHs), polyfluoroalkyl chemicals) or social stress and psychiatric disorders (anxiety, depression, psychosis, attention deficits) appear. Critical conditions treatment consists administration antidepressants antipsychotics drugs. These drugs are widely prescribed, but...

Synthetic compounds with pharmaceutical applications in brain disorders are daily designed and synthesized, well first effects but also seldom severe side effects. This imposes the search for alternative therapies based on potentials of natural compounds. The isolated from various plants arthropods venom known their antimicrobial (antibacterial, antiviral) antiinflammatory activities, more studies needed a better understanding structural pharmacological features new therapeutic...