A5056877439- Cancer Genomics and Diagnostics
- Cancer Cells and Metastasis
- Prostate Cancer Treatment and Research
- Cell death mechanisms and regulation
- Sarcoma Diagnosis and Treatment
- Colorectal Cancer Treatments and Studies
- Cancer Immunotherapy and Biomarkers
- Cervical Cancer and HPV Research
- Molecular Biology Techniques and Applications
- Cancer, Hypoxia, and Metabolism
- Cancer Research and Treatments
- CAR-T cell therapy research
- Immunotherapy and Immune Responses
- Cancer-related gene regulation
- COVID-19 Clinical Research Studies
- Lung Cancer Treatments and Mutations
- Cancer-related Molecular Pathways
- Genetic factors in colorectal cancer
- HER2/EGFR in Cancer Research
- Colorectal and Anal Carcinomas
- SARS-CoV-2 and COVID-19 Research
- Lymphoma Diagnosis and Treatment
- Virus-based gene therapy research
- SARS-CoV-2 detection and testing
- Hematopoietic Stem Cell Transplantation
National Cancer Institute
2012-2023
Center for Cancer Research
2010-2023
Science Applications International Corporation (United States)
2011-2023
ORCID
2010-2022
National Institutes of Health
2009-2021
Leidos Biomedical Research Inc. (United States)
2017-2018
Leidos (United States)
2015-2018
Target (United States)
2018
People's Hospital of Yangzhong
2014
Winship Cancer Institute
2011
Abstract Purpose: M7824 (MSB0011359C) is an innovative first-in-class bifunctional fusion protein composed of a mAb against programmed death ligand 1 (PD-L1) fused to TGFβ “trap.” Experimental Design: In the 3+3 dose-escalation component this phase I study (NCT02517398), eligible patients with advanced solid tumors received at 1, 3, 10, or 20 mg/kg once every 2 weeks until confirmed progression, unacceptable toxicity, trial withdrawal; in addition, cohort initial 0.3 dose evaluate...
We have used the chromatin immunoprecipitation technique to analyze formation of androgen receptor (AR) transcription complex onto prostate-specific antigen (PSA) and kallikrein 2 promoters in LNCaP cells. Our results show that loading holo-AR recruitment RNA polymerase II occur transiently. The cyclic nature AR assembly is also illustrated by transient association coactivators GRIP1 CREB-binding protein acetylated histone H3 with PSA promoter. Treatment cells pure antiandrogen bicalutamide...
We have used chromatin immunoprecipitation (ChIP) assay to follow transcription factor loading and monitor changes in covalent histone modifications associated with the prostate-specific antigen kallikrein (KLK2) genes response androgen antiandrogen LNCaP cells. The dynamics of testosterone (T)-induced receptor (AR) onto proximal promoters differed significantly from that distal enhancers. Significantly more holo-AR was loaded enhancers than promoters, but receptor's residence time transient...
Abstract Several variants of SARS-CoV-2 have emerged. Those with mutations in the angiotensin-converting enzyme (ACE2) receptor binding domain (RBD) are associated increased transmission and severity. In this study, we developed both antibody quantification functional neutralization assays. Analyses COVID-19 convalescent diagnostic cohorts strongly support use RBD levels as an excellent surrogate to biochemical activities. Data further revealed that samples from mRNA vaccinated individuals...
This study aimed to evaluate the clinical efficacy of percutaneous vertebroplasty (PVP) performed with a two-step fluoroscopy technique for treating thoracic osteoporotic vertebral compression fractures (OVCFs) in older patients. A retrospective analysis was conducted on and imaging data from 48 patients diagnosed OVCFs, who underwent treatment vertebroplasty(PVP) utilizing at Yangquan First People's Hospital between January 2019 2022. The assessed this procedure by analyzing Visual Analog...
Circulating cell-free (ccf) human papillomavirus (HPV) DNA may serve as a unique tumor marker for HPV-associated malignancies, including cervical cancer. We developed method to genotype and quantify circulating HPV in patients with HPV16- or HPV18-positive metastatic cancer potential disease monitoring treatment-related decision making.
Rhabdomyosarcoma (RMS) is a common pediatric soft-tissue tumor. In this study, we evaluated the efficacy and selectivity of drozitumab, death receptor DR5-targeted therapeutic antibody, in RMS preclinical models.A panel 11 cell lines was used for vitro studies. The molecular marker predictive response to drozitumab interrogated. Selected were injected into gastrocnemius muscle mice vivo assessment potency drozitumab.We report that DR5, but not DR4, persisted at high levels on surface all...
Nuclear receptors, including the androgen receptor (AR), regulate target cell transcription through interaction with auxiliary proteins to modify chromatin structure. We describe herein a novel AR-interacting protein, termed ARIP4, that has structural features typical of SNF2-like protein family. With regard Snf2 domain, closest homolog ARIP4 is ATRX protein. nuclear and comprises 1466 amino acids. It interacts AR in vitro cultured yeast mammalian cells. can be labeled 8-azido-[γ- 32 P]ATP...
Rhabdomyosarcoma (RMS) is the most common soft tissue sarcoma in children. It believed to arise from skeletal muscle progenitors, preserving expression of genes critical for embryonic myogenic development such as MYOD1 and myogenin. RMS classified embryonal, which more younger children, or alveolar, prevalent elder children adults. Despite aggressive management including surgery, radiation, chemotherapy, outcome with metastatic dismal, prognosis has remained unchanged decades. Apoptosis a...
Abstract Background There is an urgent need for a companion assay to work with mesothelin-targeted therapeutic agents and noninvasive accurate prognostication of malignant mesothelioma (MM) patients. We report the development validation blood-based megakaryocyte potentiating factor (MPF) evaluation its effectiveness prognosis in MM lung cancer Methods Using electrochemiluminescence technology, we developed sensitive MPF performed both analytical clinical validations. Further, predicting was...
Despite good initial response to chemotherapy, 30% of Ewing's sarcoma (EWS) patients with localised tumours develop recurrent disease, associated poor prognosis. The aim this study was address challenge by conducting preclinical evaluation a death receptor targeted agent in vitro and vivo, identifying predictive biomarkers. Cell viability assays, drug dose responses, immunoblots, rescue gene transfer, mice tumour models, statistical comparisons growth Kaplan–Meier survival curves. This shows...
Abstract Agents targeting the insulin-like growth factor-1 receptor (IGF1R) are in clinical development, but, despite some initial success of single agents sarcoma, response rates low with brief durations. Thus, it is important to identify markers predictive response, understand mechanisms resistance, and explore combination therapies. In this study, we found that, while associated PAX3-FKHR translocation, increased IGF1R level an independent prognostic marker for worse overall survival,...
Abstract Circulating tumor cells (CTCs) are rare cancer circulating in the bloodstream that thought to have a key role metastasis. They been associated with worse prognosis several major types. There is an intense interest molecular analysis of CTC gain information would assist treatment decisions and potentially improve disease outcome. enormous interests CTCs for castration-resistant prostate (CRPC) due nature tends metastasize bone, making them generally inaccessible obtain biopsies. In...
Abstract Circulating tumor cells (CTCs) are rare cancer in the bloodstream thought to have a key role metastasis. There enormous interests their analysis for castration-resistant prostate (CRPC) due tendency metastasize bone. In this study, we applied surface marker-independent microfluidic device CTCs capture and retrieval that is based on cell size deformability. Our was able 90% recover 30% of spike experiments. We also developed validated assays capable detecting transcript levels...
112 Background: Increased androgen receptor (AR) expression and signaling is associated with the progression of prostate cancer. Constitutively active AR splice variants (ARVs) are thought to arise following castration play a key role in progression. The most well studied variant, v567es , lacks portions ligand-binding domain, increasing full-length transcriptional activity human cancer cell lines. Tumor-derived tissue can be difficult obtain for molecular studies on patients (pts)...
Abstract Despite good initial response to cytotoxic chemotherapy, approximately 30% of Ewing's sarcoma (EWS) patients who present with localized tumors develop recurrent disease, which is associated poor prognosis. Addressing this clinical challenge requires the development targeted therapies. This study shows that many EWS cell lines are selectively sensitive a death receptor DR5 antibody and more resistant DR4 antibody. Preclinical evaluation these indicates their sensitivity human agonist...
Objective To observe the clinical effects of arthroscopic debridement combined with injection sodium hyalumnate (SH) for knees osteoarthritis (OA).Methods From Jun.2001 to Jun.2011,588 cases were analyzed,including 281 (control group) used alone and other 307 (experimental hyaluronate.Results The two groups followed-up 24 months:the excellent good rate knee joint function was 90.5％ in experimental group,but 78.3％ control group.According Lequesne index scores,the group better than months...
Abstract Agents targeting the insulin-like growth factor 1 receptor are in various stages of clinical development. Despite some initial response with single agent trials, responses limited and durations short. It is important to understand mechanics innate adaptive tumors that lead resistance. An vivo mouse model was used for development IGF1R antibody-resistant rhabdomyosarcoma tumor. All tumors, despite remarkable response, all eventually grow presence antibody. The isolated cell lines...
Abstract Circulation tumor cells are critically important to understand metastasis and may have a potential noninvasively evaluate the cancer for clinical management. The isolation characterization of CTC represent major technological challenge. While CellSeach system by J&J has shown that enumeration can be an independent predictor survival breast, prostate colon cancer, little molecular information obtained from impact on patient management or outcome. Most hormone dependent...