A5022760773- Lipid Membrane Structure and Behavior
- Protein Structure and Dynamics
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Spectroscopy and Quantum Chemical Studies
- Lipoproteins and Cardiovascular Health
- COVID-19 Clinical Research Studies
- Force Microscopy Techniques and Applications
- Cholesterol and Lipid Metabolism
- Metabolomics and Mass Spectrometry Studies
- Peroxisome Proliferator-Activated Receptors
- RNA Research and Splicing
- RNA Interference and Gene Delivery
- Liver Disease Diagnosis and Treatment
- Vitamin C and Antioxidants Research
- Nanopore and Nanochannel Transport Studies
- SARS-CoV-2 and COVID-19 Research
- Cancer, Lipids, and Metabolism
Malmö University
2017-2024
Institut Laue-Langevin
2017-2023
Institut de Biologie Structurale
2020-2023
Emerging therapeutic treatments based on the production of proteins by delivering mRNA have become increasingly important in recent times. While lipid nanoparticles (LNPs) are approved vehicles for small interfering RNA delivery, there still challenges to use this formulation delivery. LNPs typically a mixture cationic lipid, distearoylphosphatidylcholine (DSPC), cholesterol, and PEG-lipid. The structural characterization mRNA-containing (mRNA-LNPs) is crucial full understanding way which...
Arrays of nanoparticle-supported lipid bilayers (nanoSLB) are lipid-coated nanopatterned interfaces that provide a platform to study curved model biological membranes using surface-sensitive techniques. We combined scattering techniques with direct imaging, gain access sub-nanometer scale structural information on stable nanoparticle monolayers assembled silicon crystals in noncovalent manner Langmuir-Schaefer deposition. The structure supported formed the arrays via vesicle fusion was...
Cholesterol is an essential component of mammalian membranes and known to induce a series physicochemical changes in the lipid bilayer. Such include formation liquid-ordered phases with increased thickness configurational order as compared liquid-disordered phases. For saturated membranes, cholesterol molecules localize close head group-tail interface. However, presence polyunsaturated lipids was recently shown promote relocation toward inner interface between two bilayer leaflets. Here,...
Cholesterol has been shown to affect the extent of coronavirus binding and fusion cellular membranes. The severity Covid-19 infection is also known be correlated with lipid disorders. Furthermore, levels both serum cholesterol high-density lipoprotein (HDL) decrease severity, normal resuming once passed. Here we demonstrate that SARS-CoV-2 spike (S) protein interferes function lipoproteins, this dependent on cholesterol. In particular, ability HDL exchange lipids from model membranes altered...
Abstract The deuteration of biomolecules provides advanced opportunities for neutron scattering studies. For low resolution studies using techniques such as small-angle and reflection, the level a sample can be varied to match length density specific D 2 O/H O solvent mixture. This major value in structural where regions complex system highlighted, others rendered invisible. is especially useful analyses structure dynamics membrane components. In mammalian membranes, presence cholesterol...
Lipoproteins play a central role in the development of atherosclerosis. High and low-density lipoproteins (HDL LDL), known as 'good' 'bad' cholesterol, respectively, remove and/or deposit lipids into artery wall. Hence, insight lipid exchange processes between cell membranes is particular importance understanding onset cardiovascular disease. In order to elucidate impact phospholipid tail saturation presence cholesterol on these processes, neutron reflection was employed present...
Abstract High‐density lipoproteins (HDLs) are responsible for removing cholesterol from arterial walls, through a process known as reverse transport. The main protein in HDL, apolipoprotein A‐I (ApoA‐I), is essential to this process, and changes its sequence significantly alter HDL structure functions. ApoA‐I amyloidogenic variants, associated with particular hereditary degenerative disease, particularly effective at facilitating removal, thus protecting carriers cardiovascular disease....
There is a close relationship between the SARS-CoV-2 virus and lipoproteins, in particular high-density lipoprotein (HDL). The severity of coronavirus disease 2019 (COVID-19) inversely correlated with HDL plasma levels. It known that spike (S) protein binds particle, probably depleting it lipids altering function. Based on neutron reflectometry (NR) ability to efflux cholesterol from macrophages, we confirm these observations further identify preference S for specific consequent effects...
Apolipoprotein E (ApoE), an important mediator of lipid transportation in plasma and the nervous system, plays a large role diseases such as atherosclerosis Alzheimer's. The major allele variants ApoE3 ApoE4 differ only by one amino acid. However, this difference has consequences for physiological behaviour each variant. In paper, we follow (i) initial interaction lipid-free ApoE with model membranes function saturation, (ii) formation reconstituted High-Density Lipoprotein-like particles...
Atherosclerosis is the leading cause of death in west.In atherosclerosis, plaques fat and fibrous elements accumulate arteries to heart disease stroke.Current knowledge recognizes a range indicators associated with disease, including concentrations ratios different lipoprotein particles [1][2].These lipoproteins act as carriers cholesterol triglycerides, which are otherwise insoluble blood plasma.The main transporters lowdensity (LDL) high-density (HDL), cardiovascular has been shown be...
Abstract The structure of supported lipid bilayers formed on a monolayer nanoparticles was determined using combination grazing incidence X-ray and neutron scattering techniques. Ordered nanoparticle arrays assembled silicon crystal Langmuir-Schaefer deposition were shown to be suitable stable substrates for the formation curved fluid that retained lateral mobility, as by fluorescence recovery after photobleaching. A comparison between bilayer around with planar membrane flat underlying...