Background Doxorubicin (DOX) is a potent chemotherapeutic drug used against cancer but known to have adverse effects on male reproductive health. Objective This study investigated the protective of aronia (Aronia melanocarpa) DOX-induced testicular damage in rats, examining both short-term (2 weeks) and long-term (9 exposure. Methods In this study, 36 Wistar albino rats were divided into two main groups: (ST) (LT). Each group was further subdivided three control, DOX, DOX + Aronia, with 6...

Cryopreservation of buck semen is essential in animal breeding but often damages sperm viability and integrity. The Honamli breed, a hardy Turkish goat, can benefit from improved freezing techniques using antioxidants such as Trolox (T). This study explores the effects varying T concentrations on semen, assessing parameters motility, viability, membrane integrity to enhance post-thaw quality. Findings support T's potential improve extender formulations for preserving genetics.This aims...

The aim of this study was to determine the effects thymoquinone (TQ), which is most essential active compound Nigella sativa, on spermatological parameters ram semen during cryopreservation. Ejaculates were collected from five Sonmez rams using an artificial vagina and extended with Tris-based extender not containing TQ (control, 0 μg/ml TQ) 10, 25, 50 100 TQ. samples equilibrated in a + 4°C cold cabinet for 2 h. After h, loaded into 0.25 ml French straws. straws frozen by liquid nitrogen...

The objective of this study was to determine effects supplementing Tris-based semen extenders with either cholesterol-loaded cyclodextrin (CLC) or 7-dehydrocholesterol loaded (7-DCLC) plus trehalose (T) for cryopreservation ram semen. Semen collected an artificial vagina from five Merino rams (2-3 years age) during the non-breeding season. Ejaculates were pooled, divided into eight equal portions, diluted a standard extender containing: no additive (control); T (50 mM); mM) + 1.5, 2.5 3.5 mg...

The purpose of the present study was to assess effects ellagic acid and ebselen on sperm oxidative stress parameters during liquid preservation ram semen.In this experimental study, sixty ejaculates from six mature Merino rams were used. In experiment 1, diluted in base extender contained at 0 (control), 0.5, 2 mM. 2, 10, 20, 40 μM added extender. Sperm motility, viability, mitochondrial membrane potential, DNA integrity, lipid peroxidation (LPO), antioxidant potential (AOP), total...

The objective was to determine effects of gallic acid (GA) and carnosic (CA), present in carob pods rosemary extract respectively, on frozen-thawed ram spermatozoa. Thirty ejaculates were collected from five Merino rams, pooled, diluted Tris-based extender divided into equal portions containing: 0.05 or 2 mM GA; 0.2 CA; no additive (control). Extended semen equilibrated at +4°C, loaded straws, held 5 cm above liquid nitrogen for 12 min then plunged. Computer-aided sperm analysis used assess...

The aim of this study was to determine the effects trehalose and cysteine on sperm motility, viability, mitochondrial activity acrosome integrity during liquid storage Merino ram semen. Ejaculates were collected using artificial vaginas from five rams, microscopically evaluated pooled at 37 °C. semen samples diluted in a Tris-based extender, including (2 mM 4 mM), (10 25 mM) no antioxidant (control). Diluted kept tubes cooled 5 °C cold cabinet, maintained Cooled for 0, 24, 48, 72 96 h....

Our objective was to evaluate the effects of adding 50 mM trehalose (TRE), 1 cysteine (CYS), or 400 µg catalase (CAT), individually in combinations, a Tris-based semen extender and determine on fresh, chilled-equilibrated, frozen-thawed bull sperm. Ejaculates from three Holstein bulls (1-3 years old) were pooled, divided into six equal portions, put supplemented with no additive (control), TRE, CYS, CAT, TRE + CYS CAT. In chilled-equilibrated semen, motility highest CAT but lowest control (P...

<p>The carob tree (Ceratonia siliqua) grows naturally in the Mediterranean region. The empiric use of cures for their aphrodisiac properties is very common Turkey. Thus, experiment was conducted to determine effects bean extracts on some reproductive parameters male New Zealand White rabbits. During adaptation period (stage 1), 6-8 mo old rabbits were trained semen collection 30 d. At beginning treatment 2), assigned randomly 2 groups 8 animals each. For a 49 d (1 spermatogenesis...

This study aimed to investigate the effectiveness of using red pine bark tree extract (P; Pinus brutia Ten) as a TRIS extender in an attempt prevent oxidative stress bull spermatozoa after freezing. Semen specimens were obtained from Simmental bulls via artificial vagina and pooled. They separated into five diluted with Tris consisting P (200, 100, 50 25 µg/ml) free (control; C) up final concentration 16 × 106 per straw. All equilibrated for period 4 hr at temperature 4°C, following which...

Background: Most part of ram spermatozoa membrane has unsaturated fatty acids (phospholipids). Membrane structure cells is composed double ordered phospholipid layers adorned with mosaic-like protein, glycoprotein and glycolipids. Sperm freezing protocols could be negatively affected on sperm motility, viability acrosome integrity during cryopreservation. For these reasons, researchers were designed their topics led to the search for effective antioxidant systems against peroxidative damage...

We conducted this study to determine the potential cryopreservative effects of different hesperidin (vitamin P; H) doses on ram semen after freeze-thawing. Semen samples were obtained from Sönmez rams using an artificial vagina. The divided into six groups: control, 10, 50, 100, 250, and 500 µg/mL H (C, H10, H50, H100, H250, H500, respectively). At end study, sperm motility kinetic parameters, acrosome integrity (AI), mitochondrial membrane (MMP), viability, lipid peroxidation levels (LPL),...

Abstract To the best of our knowledge, no research has been conducted to test effects syringic acid (SA) on ram semen freezing within scope natural antioxidants added extenders. Therefore, this study had two main objectives. First, whether adding SA extender a protective effect and contributes positively sperm kinetic, plasma acrosome integrity, mitochondrial membrane potential, lipid peroxidation, oxidant antioxidant DNA damage parameters after thawing. Second, it was determine at what...

BACKGROUND: Carmustine is a chemotherapeutic agent that mainly used in the treatment of glioblastoma and can cause toxic effects on various organs, including liver. The white mulberry extract has anti-apoptotic anti-oxidant effects. OBJECTIVE: study aimed at investigating dried pathology, apoptosis, oxidative stress liver, as well levels serum adenosine deaminase, glutathione peroxidase, superoxide dismutase, ceruloplasmin, paraoxonase, malondialdehyde carmustine-administrated rats. METHODS:...

Amaç: Bu çalışmada dondurma-çözdürme sonrası Kangal köpeği spermatolojik parametreleri üzerine BME'nin etkisinin ortaya konulması

Aim:The purpose of this study was to investigate the effect different rate gallic acid on ram semen liquid storage in non-breeding season. Materials and Methods:In study, four Merino rams (2-3 ages) were used belonging special sheep farm Callica, Burdur/ Turkey.Ejaculates collected from using a electroejaculator, ejaculates containing spermatozoa with >80% motility concentrations higher than 1.5x10⁹ spermatozoa/ml mixed study.The divided into equal aliquots samples extended tris 0 mM...

The aim of this study was to determine the effects gallic acid (GA) on frozen-thawed goat spermatozoa. Four Honamli bucks were used at their breeding season, and ejaculates collected by an electroejaculator. Mixed semen divided into following four groups: control (0 mM), low (L; 1 medium (M; 2 high (H; 4 mM) concentration GA. All groups frozen thawed in a water bath for spermatological evaluation. lowest motility observed group (47.60 ± 5.70%) (P < 0.05), while highest viability (62.45...

JWH-018 is a synthetic cannabinoid which has been increasingly used by adolescents and adults, known to cause severe multi-organ failure. However, little about the complications toxicological effects of on reproduction system. Therefore, aim present study investigate testis spermatogenesis. Thirty CD-1 male rats were distributed into six groups, control group (C1 C2), ethanol (E1 E2), (JWH1 JWH2), administered 0.9% NaCl, %100 ethanol, (0.3 mg/kg) respectively for 9 d. We euthanized C1, E1,...

Baicalein (B) has potential antioxidant properties, but it not been tested as a ram semen extender. This study aimed to assess the impact of B on various sperm parameters and determine its influence quality after freeze-thawing process. During breeding season, ejaculates were obtained from four rams with aid an artificial vagina. The collected mixed samples divided into groups: control (C; 0), B0.5 (0.5 mM), B1 (1 B2 (2 mM). After extension, loaded 0.25 mL straws stored for 2 h at 4°C prior...

In this study, it was aimed to investigate the concentrations of macro and trace elements in seminal plasma blood serum breeding (October) non-breeding (April) seasons Merino Ram. Nineteen Rams, aged 18-24 months, were involved study. Blood (once) ejaculate samples (6 replicates) taken seasons. serum, diet Calcium, Sodium, Potassium, Magnesium, Phosphorus, Sulfur, Zinc, Selenium, Chrome, Manganese, Nickel, Molybdenum Boron determined by ICP-AES. Sodium Selenium higher (p&lt;0.05 0.001,...

In the presented study, protective role of carnosic and gallic acid on post-thaw ram sperm acrosome membrane integrity was examined, homocysteine nesfatin levels were determined.&#x0D; Six ejaculates for each used in study. Each ejaculate, split into five equal aliquots diluted with extenders including 0.05 mM (GA), 2 acid, (CA), 0.2 no additive (control) at 37 °C cooled to 5 then frozen nitrogen vapor. &#x0D; Freeze-thawed semen viability achieved 2mM (57.13± 2.38%) group statistical...

In this study, it was designed to reveal the effects of capsaicin on oxidative stress and freezability ram semen. Ejaculates were taken from Sönmez rams divided into five specimens diluted with extender at different rates (4 mM, 2 1 500 µM) without (control; C). At end sperm motility kinetic parameters, plasma membrane acrosome integrity (PMAI), mitochondrial potential (MMP), DNA damage, oxidant antioxidant parameters analyzed. A decrease observed in groups containing compared C terms...