A5075692020- Tissue Engineering and Regenerative Medicine
- Electrospun Nanofibers in Biomedical Applications
- Mesenchymal stem cell research
- MicroRNA in disease regulation
- Neonatal Respiratory Health Research
- Infant Nutrition and Health
- Angiogenesis and VEGF in Cancer
- Cancer-related molecular mechanisms research
- Immune Response and Inflammation
- 3D Printing in Biomedical Research
- Cancer Cells and Metastasis
- Vascular Procedures and Complications
- RNA Interference and Gene Delivery
- Phytochemical and Pharmacological Studies
- Central Venous Catheters and Hemodialysis
- Diabetes Treatment and Management
- Sepsis Diagnosis and Treatment
- Polyamine Metabolism and Applications
- Infectious Aortic and Vascular Conditions
- Dietary Effects on Health
- Circular RNAs in diseases
- Vascular anomalies and interventions
- Medicinal plant effects and applications
- Tumors and Oncological Cases
- Reconstructive Surgery and Microvascular Techniques
Mashhad University of Medical Sciences
2020-2022
Tabriz University of Medical Sciences
2018-2021
The emergence of numerous tissue engineering and regenerative medicine techniques cell encapsulation paves a way to heal restore the function various injured tissues mainly cardiovascular system. Here, we aimed investigate role alginate-gelatin on dynamic rat cardiomyoblasts in vitro. Rat line H9C2 were enclosed by using microspheres incubated for 7 days. MTT method was used examine viability. level genes associated with maturation MYL7, NPPA, NKX2-5, GATA4 real-time PCR. ELISA measure...
Today, many attempts have been collected in the field of tissue engineering for reconstitution injured bone marrow capacity by transplantation functional cell source. By having a three-dimensional condition, microcapsules are appropriate candidates cells to target sites. Here, we examined effect alginate–gelatin on maturation human myelomonocytic line U937 after 7 days vitro. were encapsulated mixture and cultured days. Trypan blue staining was used show survival rate. Morphological changes...
There are still challenges regarding c-kit+ cells' therapeutic outcome in the clinical setting. Here, we examined cell effect on alleviation of asthma by modulating miRNAs expression.To induce asthma, male rats were exposed to ovalbumin. Bone marrow-derived cells enriched MACS. Animals classified into four groups (6 each). Control received PBS intratracheally; Ovalbumin-sensitized intratracheally containing 3×105 and c-kit- cells. Cells stained with Dil fluorescent dye track vivo condition....
Purpose: Diabetes mellitus, especially type 2, is conceived as a devastating chronic metabolic disease globally. Due to the existence of an extensive vascular network in pulmonary tissue, it suggested that lungs are sensitive diabetic condition like other tissues. This study was designed address possible effect 2 diabetes mellitus on promotion pathological changes via injury. Methods: Sixteen male Wistar rats were randomly allocated two Control and T2D groups. To induce diabetes, received...
Purpose: Here, we investigated the angiogenic potential of endothelial progenitor cells juxtaposed with mesenchymal stem (MSCs) inside alginate-gelatin microspheres stromal derived factor-1α (SDF-1 α) for 7 days. Methods: Six encapsulated groups were allocated including (EPCs), EPCs/SDF-1α, MSCs, MSCs/SDF-1α, EPCs+MSCs and EPCs+MSCs/SDF-1α. Cells a mixture 1% alginate 2% gelatin hydrogel. Cell survival was examined by MTT assay. Endothelial differentiation determined flow cytometry ELISA....
Purpose: Today, there is an urgent need to develop a three-dimentional culture systems mimicking native in vivo condition order screen potency of drugs and possibly any genetic alterations tumor cells. Due the existence limitations animal models, development three dimensional highly recommended. To this end, we encapsulated human colon adenocarcinoma cell line HT29 with alginate-poly-L-lysine (Alg-PLL) microspheres rate epithelial-mesenchymal transition was monitored. Methods: Cells were...
The emergence of an inflammatory condition such as asthma could affect the therapeutic potential stem cells. Synopsis previous documents yielded controversial outcomes, leading to a limitation cell‐based therapy in clinical setting. This study aimed assess impact asthmatic serum on MSCs aging and dynamic growth vitro. Rats were divided into control groups randomly. change was induced using OVA sensitization. structural changes are monitored by conventional Haematoxylin‐Eosin staining....
Abstract Objective The current experiment aimed to assess the impact of detergents such as 3% Triton X-100, 1% peracetic acid, Tween-20, and SDS in combination with Trypsin–EDTA on acellularization ovine aortae after 7 days. Results Hematoxylin–Eosin staining showed an appropriate rate aortae, indicated by a lack cell nuclei tunica media layer. DAPI confirmed vascular wall being exposed chemical enzymatic solutions. Verhoeff-Van Gieson that elastin fibers were diminished acellular samples...
CRISPR/Cas9 is a powerful gene-editing technology. Extensive scientific data exist that the system can target small, non-coding, active RNA molecules, including microRNAs (miRNAs). miRNAs have been recognized as key regulators of different cell biological processes, such modulation fibrosis and cardiac hypertrophy, well regulation cardiomyocytes. Also, it has demonstrated strongly affect organ evolution, concentration involve differentiation, development, function organs. In addition,...
Background: The need for on-demand biological products has been raised during the last decades. To prepare ready-to-use organ-related products, it necessitates a bulk cell reservoir. In this regard, stem cells, especially mesenchymal have numerous therapeutic properties in tissue repair. Therefore, advent of novel banking systems is inevitable to maintain cells ready-to-use. Objectives: current study, cryoprotective effects alginate-gelatin were investigated on human after seven days....
Abstract Background There are still challenges regarding c-kit + cells therapeutic outcome in the clinical setting. Here, we examined of effect on alleviation asthma by modulating miRNAs expression. Methods To induce asthma, male rats were exposed to ovalbumin. Bone marrow-derived enriched MACS. Animals classified into four groups (each 6 rats). Control received PBS intratracheally; Ovalbumin-sensitized intratracheally containing 3×10 5 and - cells. Cells stained with Dil fluorescent dye...
Traumatic carotid artery jugular vein fistula is a rare entity that not usually detected during the acute injury phase. We presented 1 cases of traumatic carotid–jugular fistula. A 52 years old man was referred to us because an expanding neck hematoma. There 1×1 cm ulcer with clot in zone II right and thrill palpated. clamped proximal distal site (Arteriovenous fistula), then divided primarily repaired (transversely) 7-0 prolene suture. placed two hemovac drains subcutaneous skin covered...