A5004795117- Parasitic Infections and Diagnostics
- Malaria Research and Control
- Trypanosoma species research and implications
- Invertebrate Immune Response Mechanisms
- CRISPR and Genetic Engineering
- Bacteriophages and microbial interactions
- Aquaculture disease management and microbiota
- Microbial infections and disease research
- Plant Virus Research Studies
- Genomics and Phylogenetic Studies
- Genetic Neurodegenerative Diseases
- Genomics and Chromatin Dynamics
- Toxoplasma gondii Research Studies
- Amyotrophic Lateral Sclerosis Research
- Gene expression and cancer classification
- Research on Leishmaniasis Studies
- Parasites and Host Interactions
- Mosquito-borne diseases and control
- Parasite Biology and Host Interactions
- Sulfur Compounds in Biology
- Chromosomal and Genetic Variations
- Prion Diseases and Protein Misfolding
- Insect Resistance and Genetics
- RNA and protein synthesis mechanisms
- Insect symbiosis and bacterial influences
Ludwig-Maximilians-Universität München
2022-2025
Inserm
2016-2024
Institut Pasteur
2016-2024
Centre National de la Recherche Scientifique
2016-2024
Ciência Viva
2024
Université Paris Cité
2022-2024
Sorbonne Université
2020-2024
Université Paris Sciences et Lettres
2024
Institut Curie
2024
Université de Montpellier
2024
Plasmodium falciparum is the deadliest malaria parasite species, accounting for vast majority of disease cases and deaths. The virulence this reliant upon mutually exclusive expression cytoadherence proteins encoded by 60-member var gene family. Antigenic variation multigene family serves as an immune evasion mechanism, ultimately leading to chronic infection pathogenesis. Understanding regulation mechanism antigenic key developing new therapeutic control strategies. Our study uncovers a...
Monoallelic expression of the var multigene family enables immune evasion malaria parasite Plasmodium falciparum in its human host. At a given time only single member 60-member gene is expressed at discrete perinuclear region called 'var site'. However, mechanism counting remains ill-defined. We hypothesize that activation factors associating specifically with site play key role this process. Here, we investigate GC-rich non-coding RNA (ncRNA) composed 15 highly homologous members. genes are...
Abstract Antigenic variation is an immune evasion strategy used by many different pathogens. It involves the periodic, non-random switch in expression of antigens throughout infection. How observed hierarchy antigen achieved has remained a mystery 1,2 . A key challenge uncovering this process been inability to track transcriptome changes and potential genomic rearrangements individual cells during event. Here we report establishment highly sensitive single-cell RNA sequencing approach for...
Abstract Nucleic acid interactome data, such as chromosome conformation capture data and RNA–DNA are currently analyzed via pipelines that must be rerun for each new parameter set. A more dynamic approach is desirable since the optimal set commonly unknown ahead of time rerunning a time-consuming process. We have developed an fast enough to process on-the-fly using sparse prefix sum index. With this index, we created Smoother, flexible, multifeatured visualization analysis tool allows...
While often undetected and untreated, persistent seasonal asymptomatic malaria infections remain a global public health problem. Despite the presence of parasites in peripheral blood, no symptoms develop. Disease severity is correlated with levels infected red blood cells (iRBCs) adhering within vessels. Changes iRBC adhesion capacity have been linked to infections, however how this occurring still unknown. Here, we present evidence that RNA polymerase III (RNA Pol III) transcription...
Non-coding RNAs (ncRNAs) are emerging regulators of immune evasion and transmission Plasmodium falciparum . RUF6 is an ncRNA gene family that transcribed by RNA polymerase III but actively regulates the Pol II–transcribed var virulence family. Understanding how connects to downstream effectors lacking. We developed RNA-directed proteomic discovery (ChIRP-MS) protocol identify in vivo ncRNA–protein interactions. The interactome was purified with biotinylated antisense oligonucleotides....
While often undetected and untreated, persistent seasonal asymptomatic malaria infections remain a global public health problem. Despite the presence of parasites in peripheral blood, no symptoms develop. Disease severity is correlated with levels infected red blood cells (iRBCs) adhering within vessels. Changes iRBC adhesion capacity have been linked to infections, however how this occurring still unknown. Here, we present evidence that RNA polymerase III (RNA Pol III) transcription...
Many neurodegenerative disorders display protein aggregation as a hallmark, Huntingtin and TDP-43 aggregates being characteristic of Huntington disease amyotrophic lateral sclerosis, respectively. However, whether these cause the diseases, are secondary by-products, or even have protective effects, is matter debate. Mutations in both human proteins can modulate structure, number type aggregates, well their toxicity. To study role cellular fitness, we expressed highly tractable unicellular...
While often undetected and untreated, persistent seasonal asymptomatic malaria infections remain a global public health problem. Despite the presence of parasites in peripheral blood, no symptoms develop. Disease severity is correlated with levels infected red blood cells (iRBCs) adhering within vessels. Changes iRBC adhesion capacity has been linked to infections, however how this occurring still unknown. Here we present evidence that RNA polymerase III (RNA Pol III) transcription...
While often undetected and untreated, persistent seasonal asymptomatic malaria infections remain a global public health problem. Despite the presence of parasites in peripheral blood, no symptoms develop. Disease severity is correlated with levels infected red blood cells (iRBCs) adhering within vessels. Changes iRBC adhesion capacity have been linked to infections, however how this occurring still unknown. Here we present evidence that RNA polymerase III (RNA Pol III) transcription...
Abstract The eukaryotic nucleus exhibits a highly organized 3D genome architecture, with RNA transcription and processing confined to specific nuclear structures. While intra-chromosomal interactions, such as promoter-enhancer dynamics, are well-studied, the role of inter-chromosomal interactions remains poorly understood. Investigating these in mammalian cells is challenging due large sizes need for deep sequencing. Additionally, transcription-dependent topologies mixed cell populations...
ABSTRACT Heterochromatin is essential in all eukaryotes to maintain genome integrity, long-term gene repression and help chromosome segregation during mitosis. However, heterochromatin regions must be restricted by boundary elements avoid its spreading over actively transcribed loci. In Plasmodium falciparum , facultative important regulate parasite virulence, antigenic variation transmission. the underlying molecular mechanisms regulating repressive remain unknown. To investigate this...
Abstract While often undetected and untreated, persistent seasonal asymptomatic malaria infections remain a global public health problem. Despite the presence of parasites in peripheral blood, no symptoms develop. Disease severity is correlated with levels infected red blood cells (iRBCs) adhering within vessels. Changes iRBC adhesion capacity have been linked to infections, however how this occurring still unknown. Here we present evidence that RNA polymerase III (RNA Pol III) transcription...