A5065405408- Cellular Mechanics and Interactions
- Glycosylation and Glycoproteins Research
- 3D Printing in Biomedical Research
- Skin and Cellular Biology Research
- Peptidase Inhibition and Analysis
- Polymer Surface Interaction Studies
- Monoclonal and Polyclonal Antibodies Research
- Cancer Cells and Metastasis
- Proteoglycans and glycosaminoglycans research
- Innovative Microfluidic and Catalytic Techniques Innovation
- Carbohydrate Chemistry and Synthesis
University of California, Berkeley
2018-2022
Peking University
2014-2018
Beijing National Laboratory for Molecular Sciences
2014
Center for Life Sciences
2014
Abstract Although it has been well known that dynamic changes in glycosylation are associated with tumor progression, remains challenging to selectively visualize the cancer glycome vivo. Herein, a strategy for targeted imaging of tumor‐associated glycans by using ligand‐targeted liposomes encapsulating azidosugars is described. The intravenously injected liposomal nanoparticles bound cancer‐cell‐specific receptors and installed azides into melanoma xenograft mouse model tissue‐specific...
Metabolic labeling of glycans with bioorthogonal reporters has been widely used for glycan imaging and glycoproteomic profiling. One the intrinsic limitations metabolic is lack cell-type selectivity. The recently developed liposome-assisted reporter (LABOR) strategy provides a promising means to overcome this limitation, but mechanism LABOR not investigated in detail. In work, we performed mechanistic study on explored its multiplexing capability. Our studies support an endocytosis-salvage...
Intrinsically disordered proteins (IDPs) play central roles in numerous cellular processes. While IDP structure and function are often regulated by multisite phosphorylation, the biophysical mechanisms linking these post-translational modifications to remain elusive. For example, intrinsically C-terminal sidearm domain of neurofilament heavy subunit (NFH-SA) forms a dense brush along axonal NF backbones is subject extensive serine phosphorylation. Yet, insight into relationship between...
Abstract Although it has been well known that dynamic changes in glycosylation are associated with tumor progression, remains challenging to selectively visualize the cancer glycome vivo. Herein, a strategy for targeted imaging of tumor‐associated glycans by using ligand‐targeted liposomes encapsulating azidosugars is described. The intravenously injected liposomal nanoparticles bound cancer‐cell‐specific receptors and installed azides into melanoma xenograft mouse model tissue‐specific...
Biophysical cues in the extracellular matrix (ECM) regulate cell behavior a complex, nonlinear, and interdependent manner. To quantify these important regulatory relationships gain comprehensive understanding of mechanotransduction, there is need for high-throughput platforms that enable parallel culture analysis cells various conditions. Here we describe multiwell hyaluronic acid (HA) platform which are cultured on combinatorial arrays hydrogels spanning range elasticities adhesivities. Our...