A5050281126- Plant Molecular Biology Research
- Polysaccharides and Plant Cell Walls
- Plant Reproductive Biology
- Plant Gene Expression Analysis
- Tree Root and Stability Studies
- Plant Pathogens and Fungal Diseases
- Molecular Biology Techniques and Applications
- Advanced Cellulose Research Studies
- Forest ecology and management
- Plant tissue culture and regeneration
- Ecology and Conservation Studies
- Plant Water Relations and Carbon Dynamics
- Genomics and Phylogenetic Studies
- Ubiquitin and proteasome pathways
- Plant responses to water stress
- Congenital heart defects research
- Cell Image Analysis Techniques
- Mitochondrial Function and Pathology
- Plant Diversity and Evolution
- ATP Synthase and ATPases Research
- Plant and Fungal Interactions Research
- RNA Research and Splicing
- Protein Hydrolysis and Bioactive Peptides
- RNA modifications and cancer
- Polysaccharides Composition and Applications
The University of Melbourne
2013-2024
Ecosystem Sciences
2023
Melbourne Water
2015
Eucalypts are the world's most widely planted hardwood trees. Their outstanding diversity, adaptability and growth have made them a global renewable resource of fibre energy. We sequenced assembled >94% 640-megabase genome Eucalyptus grandis. Of 36,376 predicted protein-coding genes, 34% occur in tandem duplications, largest proportion thus far plant genomes. also shows highest diversity genes for specialized metabolites such as terpenes that act chemical defence provide unique...
Abstract Background NAC domain transcription factors initiate secondary cell wall biosynthesis in Arabidopsis fibres and vessels by activating numerous transcriptional regulators biosynthetic genes. family member SND2 is an indirect target of a principal regulator fibre formation, SND1. A previous study showed that overexpression produced cell-specific increase thickness stems, the protein was able to transactivate cellulose synthase8 ( CesA8 ) promoter. However, full repertoire genes...
Summary Cellulose microfibrils are the major structural component of plant secondary cell walls. Their arrangement in primary walls, and its consequent influence on expansion cellular morphology, is directed by cortical microtubules; cylindrical protein filaments composed heterodimers α‐ β‐tubulin. In walls woody stems orientation cellulose influences strength flexibility wood, providing physical support that has been instrumental vascular colonization troposphere. Here we show a Eucalyptus...
Fasciclin-like arabinogalactan protein (FLA) families have been identified and characterised in key plant species, with some members exhibiting functional specialization. Here we identify the FLA family of Eucalyptus grandis, investigate roles three single-FAS domain FLAs, particular focus on secondary cell-wall formation wood properties. We use various in-silico approaches to characterise E. grandis genome perform phylogenetic comparisons other species. For testing including...
We used sector analysis to study cambium development and dynamics test whether fundamental developmental functional differences exist between cambial initials as true 'stem cells' more differentiated mother cells. In many higher plants, a cylindrical lateral meristem, the vascular cambium, forms along plant axis. Most notably in stems of perennial tree species, this meristem gives rise xylem (wood) towards inside trunk phloem (bark) outside. As such, is responsible for production most...
REALLY INTERESTING NEW GENE (RING) proteins play important roles in the regulation of many processes by recognizing target for ubiquitination. Previously, we have shown that expression PtaRHE1, encoding a Populus tremula × alba RING-H2 protein with E3 ubiquitin ligase activity, is associated tissues undergoing secondary growth. To further elucidate role PtaRHE1 vascular tissues, undertaken reverse genetic analysis poplar. Within stem and its corresponding are expressed predominantly phloem....
Climatic and edaphic conditions over geological timescales have generated enormous diversity of adaptive traits high speciation within the genus Eucalyptus (L. Hér.). Eucalypt species occur from rainfall to semi-arid zones tropics latitudes as 43°S. Despite several morphological metabolomic characterizations, little is known regarding gene expression differences that underpin in tolerance environmental change. Using contrasting taxonomy, morphology physiology (E. globulus E. cladocalyx),...
Large-scale functional analysis of genes and transgenes suspected to be involved in wood development trees is hindered by long generation times, low transformation regeneration efficiencies difficulties with phenotypic assessment traits, especially those that appear late a tree’s development. To avoid such obstacles many researchers have turned model plants as Arabidopsis thaliana (L.) Heynh., Zinnia elegans Jacq. Nicotiana ssp., or focused their attention on vitro formation systems vivo...
In an attempt to devise a method for the rapid creation of somatic transgenic wood sectors sufficient size that would allow us detect and analyse altered characteristics within them, we have explored manual wounding subsequent infection with Agrobacterium dormant lateral buds in poplar. Following treatment transformation 35S-GUS construct, frequent stable was found form distinct specific GUS staining patterns outer cortex, cambial region (including primary secondary xylem phloem) pith....
Secondary stem growth in trees and associated wood formation are significant both from biological commercial perspectives. However, relatively little is known about the molecular control that governs their development. This part due to physical, resource time limitations often with study of secondary processes. A number vitro techniques have been used involving either plant or whole system woody non-woody species. questions applicability for processes, recalcitrance certain species labor...
Background NAC and MYB transcription factors (TFs) have been shown to play prominent roles in the regulation of plant developmental processes. Two Arabidopsis thaliana domain TFs (AtSND2, AtSND3) one TF (AtMYB103) were be downstream targets two master regulators xylem fibre cell development, NST1 SND1 [1,2]. These able induce expression a GUS reporter gene under control AtCesA8 promoter [3], indicating that they may involved cellulose biosynthesis secondary walls A. fibres. It is...
Abstract Key message Our Induced Somatic Sector Analysis and protein–protein interaction experiments demonstrate that Eucalyptus grandis IAA13 regulates xylem fibre vessel development, potentially via EgrIAA13 modules involving ARF2, ARF5, ARF6 ARF19. Auxin is a crucial phytohormone regulating multiple aspects of plant growth differentiation, including regulation vascular cambium activity, xylogenesis its responsiveness towards gravitropic stress. Although the these biological processes...
Secondary stem growth in trees and associated wood formation are significant both from biological commercial perspectives. However, relatively little is known about the molecular control that governs their development. This part due to physical, resource time limitations often with study of secondary processes. A number vitro techniques have been used involving either plant or whole system woody non-woody species. questions applicability for processes, recalcitrance certain species labor...
Abstract The products of secondary xylem are significant biological and commercial importance, as a result, the biology growth how intrinsic extrinsic factors influence this process have been subject intense investigation. Studies into range in scale from cellular to forest stand level, with phenotypic analyses often involving assessment traits relating cell morphology wall chemical composition. While numerous techniques currently available for samples containing abundant amounts tissue,...
Background Cellulose is an important biopolymer produced by all plants and used in a number of different industries, including for pulp paper production. deposited into the plant cell wall large membranebound protein complex, which composed cellulose synthase (CESA) proteins. The content pattern deposition walls highly variable depending on function cell. All cells have thin primary wall, but types, xylem cells, also deposit secondary to give these tissues mechanical strength required...
Abstract Key message Cortical microtubule arrays are the primary mechanism for guiding re-orientation of cellulose microfibrils and determining MFA in secondary cell wall wood fibre tracheid cells reaction wood. Microtubules directly indirectly involved synthase complexes (CSCs) through plasma membrane. The angle deposition is a critical response to environmental signals and/or stress conditions, particularly crucial during formation, process which woody plants deposit additional material...
Detailed knowledge of the tissue specificity gene expression is central importance not only for our understanding developmental processes during wood formation, but also a prerequisite deliberate manipulation xylogenesis candidate genes. Today, much about specific based on annual model plants in part because perennial tree systems are often seen as too cumbersome detailed promoter studies. Here we address this issue novel way with investigation number well characterised cellulose synthase...