A5044505827- Porphyrin Metabolism and Disorders
- Folate and B Vitamins Research
- Heme Oxygenase-1 and Carbon Monoxide
- Glycosylation and Glycoproteins Research
- Monoclonal and Polyclonal Antibodies Research
- Enzyme Structure and Function
- Chemical Synthesis and Analysis
- Cell Adhesion Molecules Research
- Peptidase Inhibition and Analysis
- Enzyme Production and Characterization
- Protein Structure and Dynamics
- Cellular transport and secretion
- Protease and Inhibitor Mechanisms
- Lipid Membrane Structure and Behavior
- Immune Cell Function and Interaction
- Mass Spectrometry Techniques and Applications
- Digestive system and related health
- RNA and protein synthesis mechanisms
- Neonatal Health and Biochemistry
- Marine Sponges and Natural Products
- Pharmaceutical industry and healthcare
- Xenotransplantation and immune response
- T-cell and B-cell Immunology
- Biochemical and Structural Characterization
- Cholesterol and Lipid Metabolism
The Geneva Association
2019-2024
Novartis Institutes for BioMedical Research
2004-2019
Novartis (Switzerland)
2001-2019
Novartis Foundation
2002
University of Freiburg
2002
University of Virginia
1995-2000
Medical University of South Carolina
1997
City of Hope
1997
City Of Hope National Medical Center
1997
McCormick (United States)
1995-1996
A synthetic, 28-residue peptide derived from the calmodulin-binding sequence of plasma membrane Ca2+ pump (C28W) inhibits ATPase activity a calpain-produced, truncated fragment enzyme. The fragment, which has lost domain, molecular mass 124 kDa and is fully active in absence calmodulin. Replacement Trp-8 by an Ala decreases overall inhibitory activity, while replacement with Tyr increases it. However, at very low concentrations effect disappears. synthetic been made photoactivatable...
Abstract Calpain proteolysis of the plasma membrane Ca 2+ pump removes a C‐terminal 14‐kDa portion which includes calmodulin‐binding domain. This produces fully activated 124‐kDa fragment, can be inhibited by synthetic versions The inhibition is strongest when Trp‐8 in latter domain replaced Tyr residue (Falchetto, R., Vorherr, T., Brunner, J., & Carafoli, E., 1991, J. Biol. Chem. 266 , 2930–2936). In present study, N‐terminus 28‐residue was acetylated with 3 H‐acetic anhydride, and Phe...
Adhesive interactions between neurons and extracellular matrix (ECM) play a key role in neuronal pattern formation. The prominent played by the protein tenascin/cytotactin development of nervous system, tied to its abundance, led us speculate that brain may contain yet unidentified tenascin receptors. Here we show cell adhesion molecule contactin/F11, member immunoglobulin(Ig)-superfamily, is surface ligand for system. Through affinity chromatography membrane glycoproteins from chick on...
Summary Aspergillus fumigatus secretes a serine alkaline protease (ALP) and metalloprotease (MEP) when the fungus is cultivated in presence of collagen as sole nitrogen carbon source. The gene encoding ALP was isolated characterized previously. We report here cloning sequencing MEP. Genomic cDNA clones were from A. libraries using synthetic oligonucleotides probes. Stretches deduced amino acid sequence found to be agreement with N ‐terminal MEP internal peptide sequences. enzyme contains...
Fusion of influenza viruses with target membranes is induced by acid and involves complex changes in the viral fusion protein hemagglutinin (HA) contact sites between (Stegmann, T., White, J. M., Helenius, A. (1990) EMBO 9, 4231-4241). At 0 degrees C, a first, kinetically distinct step, irreversibly adhere to viruses. itself starts only after lag-phase several minutes (X-31 strain viruses) or raising temperature (PR8/34 viruses). We now provide evidence that initial conformational change...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTMapping of functional domains in the plasma membrane calcium pump using trypsin proteolysisE. Zvaritch, Peter James, Thomas Vorherr, Rocco Falchetto, N. Modyanov, and Ernesto CarafoliCite this: Biochemistry 1990, 29, 35, 8070–8076Publication Date (Print):September 4, 1990Publication History Published online1 May 2002Published inissue 4 September 1990https://pubs.acs.org/doi/10.1021/bi00487a012https://doi.org/10.1021/bi00487a012research-articleACS...
The development of a quantification method for monoclonal antibodies in serum has been accomplished by high-performance liquid chromatography multiple reactions monitoring mass spectrometry. A human antibody (HmAb) was used as the model protein and validation. peptide from CDR3-region its heavy chain selected quantifying entire mAb. This signature served template internal standard. Prior to spectrometric analysis approximately 50% total content removed albumin depletion. accuracy ranged...
We describe a synthetic approach toward the rapid modification of phenyl-indolyl maleimides and discovery potent Jak3 inhibitor 1 with high selectivity within Jak kinase family. provide rationale for this unprecedented based on X-ray crystal structure an analogue bound to ATP-binding site Jak3. While equally compared Pfizer pan CP-690,550 (2) in enzymatic assay, compound was found be 20-fold less cellular assays measuring cytokine-triggered signaling through cytokine receptors containing...
The identification of a natural ligand the orphan chemoattractant receptor GPR15 provides mechanistic insight into migration lymphocytes in skin.
The stimulation of the purified human erythrocyte calcium pump by acidic phospholipids was investigated using synthetic peptides corresponding to a putative phospholipid‐responsive domain [Zvaritch, E., James, P., Vorherr, T., Falchetto, R., Modyanov, N. & Carafoli, E. (1990) Biochemistry 29 , 8070–8076] and calmodulin‐binding pump. interfered with activation enzyme phosphatidylserine phosphatidic acid in competition assays. peptide found be most efficient antagonist. Direct binding...
The extracellular acid protease of Candida tropicalis was purified from the supernatant fraction culture medium containing bovine serum albumin as nitrogen source and NH 2 ‐terminal amino (aa) sequence protein determined. gene for (ACP) isolated using a pool synthetic oligonucleotides probe segment deduced aa found to be in agreement with protein. ACP is similar proteases pepsin family. nucleotide 5′ portion this revealed coding 60 residue propeptide two Lys—Arg pairs that have been...
Lactase-phlorizin hydrolase was isolated by immunoadsorption chromatography from rabbit brush-border membrane vesicles. Inactivation of the enzyme with [3H]conduritol-B-epoxide, a covalent active site-directed inhibitor, labeled glutamates at positions 1271 and 1747. Glu1271 assigned to lactase, Glu1747 phlorizin activity. In contrast, nucleophiles in sites sucrase-isomaltase are aspartates (Asp505 Asp1394). Asp505 is part isomaltase site localized on larger subunit, which carries anchor...
An Aspergillus fumigatus extracellular alkaline protease (ALP) which is an enzyme of the subtilisin family a potential virulent factor fungus. The gene encoding ALP was isolated from genomic library made DNA A. isolate. nucleotide sequence this compared to that cDNA oryzae and mature protein. Mature contains 282 amino acids encoded by three exons. pre-proenzyme has leader 121 acids.
Candida parapsilosis secretes an inducible acid protease (ACP) when cultivated in the presence of bovine serum albumin as sole nitrogen source. In order to clone ACP gene C. parapsilosis, a genomic library was screened with tropicalis probe. Two different ORFs, ACPR and ACPL, were found hybridize ACP. contained DNA sequence agreement N-terminal amino isolated from culture supernatants. shown be expressed functional mutant (acp) SDS-PAGE protein product showed same mobility secreted by...
Acute hepatic porphyria (AHP) is a group of rare genetic diseases heme biosynthesis resulting in severe neurovisceral attacks and chronic complications that negatively impact patients' well-being. This study evaluated the impacts AHP on physical emotional health from global perspective. Adult patients United States, Italy, Spain, Australia, Mexico, Brazil with >1 attack within past 2 years or receiving intravenous hemin and/or glucose for prevention completed an online survey assessing...
The plant amino acid, mimosine, is an extremely effective inhibitor of DNA replication in mammalian cells (Mosca, P. J., Dijkwel, A., and Hamlin, J. L.(1992) Mol. Cell. Biol. 12, 4375-4383). Mimosine appears to prevent the formation forks at early-firing origins when delivered approaching G1/S boundary, blocks added S phase after a lag ∼2.5 h. We have shown previously that [3H]mimosine can be specifically photo-cross-linked both vivo vitro 50-kDa polypeptide (p50) Chinese hamster ovary (CHO)...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTStudy of calmodulin binding to the alternatively spliced C-terminal domain plasma membrane calcium pumpFelix Kessler, Rocco Falchetto, Roger Heim, Ruedi Meili, Thomas Vorherr, Emanuel E. Strehler, and Ernesto CarafoliCite this: Biochemistry 1992, 31, 47, 11785–11792Publication Date (Print):December 1, 1992Publication History Published online1 May 2002Published inissue 1 December...