A5051786136- Fermentation and Sensory Analysis
- Fungal and yeast genetics research
- Yeasts and Rust Fungi Studies
- Microbial Metabolic Engineering and Bioproduction
- Biofuel production and bioconversion
- Microbial Inactivation Methods
- Probiotics and Fermented Foods
- Antifungal resistance and susceptibility
- Polysaccharides and Plant Cell Walls
- Meat and Animal Product Quality
- Adsorption and biosorption for pollutant removal
- Thermochemical Biomass Conversion Processes
- Food Quality and Safety Studies
- Polyamine Metabolism and Applications
- Horticultural and Viticultural Research
- Genomics and Phylogenetic Studies
- Plant Pathogens and Fungal Diseases
- Polysaccharides Composition and Applications
- Enzyme Structure and Function
- Listeria monocytogenes in Food Safety
- Plant nutrient uptake and metabolism
- Transgenic Plants and Applications
- Lignin and Wood Chemistry
- Coffee research and impacts
- Natural Products and Biological Research
University of Manchester
2024
Universidad Complutense de Madrid
2005-2017
Universidad San Pablo CEU
2014-2017
Universidad Politécnica de Madrid
2015
Instituto Gulbenkian de Ciência
1977-1986
Universidad de Extremadura
1982
Centro de Implantología Cirugía Oral y Maxilofacial
1982
During the last years, several reports described an apoptosis-like programmed cell death process in yeast response to different environmental aggressions. Here, evidence is presented that hyperosmotic stress caused by high glucose or sorbitol concentrations culture medium induces Saccharomyces cerevisiae a accompanied morphological and biochemical indicators of apoptotic death, namely chromatin condensation along nuclear envelope, mitochondrial swelling reduction cristae number, production...
The killer toxin from Pichia membranifaciens CYC 1106, a yeast isolated fermenting olive brines, binds primarily to the (1-->6)-beta-D-glucan of cell wall sensitive (Candida boidinii IGC 3430). was purified walls C. by alkali and hot-acetic acid extraction, procedure which solubilizes glucans. major fraction receptor activity remained with alkali-insoluble (1-->6)-beta- (1-->3)-beta-D-glucans. chemical (gas-liquid chromatography) structural (periodate oxidation, infrared spectroscopy, (1)H...
Yeasts were isolated from spontaneous fermentations of olives in brines. Ascomycetous species dominated the yeast flora (>90%) and among them Pichia membranae‐faciens related species. Some components tested as substrates for growth. Killer activity was observed approximately half isolates, wider spectra displayed by strains anomala.
The killer properties of yeasts isolated from olive brines were examined in the absence and presence sodium chloride concentrations up to 6% (wt/vol). An apparent enhancement killing action as salt concentration increased, well changes spectra activity against selected target strains, was observed a few strains. Culture filtrates strains grown at different NaCl (0, 3, or [wt/vol]) tested sensitive cultivated under same conditions. While sensitivity strain greatly increased salt, no...
Aims: In this work the microflora present in kefir, a fermented milk product, was studied together with effect of kefir administration on different groups indigenous bacteria mouse bowel. Methods and Results: Kefir composed lactic acid bacteria, acetic yeasts. Yeast population Saccharomyces cerevisiae, S. unisporus, Candida Kluyveromyces marxianus K. lactis. The streptococci levels treated mice increased by 10-fold sulfite-reducing clostridia decreased 100-fold. number significantly....
The transport of maltose in Saccharomyces cerevisiae has been generally accepted as a H+-sugar symport, with stoichiometrical ratio 1:1. A simultaneous exit K+ from the cells initial uptake reported previously. By using K+-selective electrode and radioactive maltose, we were able to measure 1 mol K+/mol taken up by first 10-15 s. This is pH-independent. So, protons non-buffered cell suspension or buffered one can be used rates uptake. We have pH study effect external respectively on kinetic...
Aims: The aims of this study were to characterize the cell wall binding site Debaryomyces hansenii killer toxin provide a simple purification method and determine some characteristics toxin. Methods Results: Various linear (1→6)‐β‐D‐glucans different origins effective competitive inhibitors action. Periodate oxidation 1H‐NMR was used receptor nature. Affinity chromatography on pustulan‐Sepharose column purify D. toxin, probably 23‐kDa protein. character cureless. Conclusions: investigation...
Seven yeasts strains have been isolated from sewage sludge. Also six samples of compost with different sieving, composting times and origins, analysed. Apparently, processes negatively affect the viability yeasts, as none could be samples. The margins tolerance to Cd, Cu Zn determined. physiological response metals was similar in all species studied, general, kinetic parameters (mu lag) were affected. Metal uptake ability also studied inter- intra-specific heterogeneity detected, thus...
We propose a model, based on the Gompertz equation, to describe growth of yeasts colonies agar medium. This model presents several advantages: (i) one equation describes colony growth, which previously needed two separate ones (linear increase radius and squared radius); (ii) similar can be applied total viable cells, area or radius, because number cells in mature is proportional their area; (iii) its parameters estimate cell yield, concentration that triggers limitation effect this specific...
The transport systems for glucose present in Candida utilis cells, growing batch and continuous cultures on several carbon sources, have been studied. Two different were found: a proton symport facilitated diffusion system. high-affinity (Km about 15 microM) transported one per mole of was partially constitutive, appearing cells grown gluconeogenic substrates such as lactate, ethanol glycerol. It also induced by concentrations up to 0.7 mM repressed higher ones. level repression depended the...
The amplification by PCR of the Intergenic Spacer region (IGS) rDNA followed Restriction Fragment Length Polymorphism (RFLP) analysis was evaluated as a potential method for identification Debaryomyces hansenii among other yeast species that frequently contaminate Intermediate-Moisture Foods (IMFs). For first rapid differentiation at level, determination IGS-PCR fragment size found to be useful approach. digestion this with enzymes HhaI, HapII and MboI resulted in specific patterns permit D....
In this work we analyze the spoiling potential of Meyerozyma guilliermondii in yogurt. The analysis was based on contaminated samples sent to us by an industrial laboratory over two years. All plain and fruit yogurt packages were heavily yeasts, but only last ones, containing fermentable sugars besides lactose, spoiled gas swelling. These strains unable grow ferment lactose (as type strain); they did lactate plus galactose, fermented glucose sucrose, galactose (weakly), not compete with...