Allostery in proteins influences various biological processes such as regulation of gene transcription and activities enzymes cell signaling. Computational approaches for analysis allosteric coupling provide inexpensive opportunities to predict mutations design small-molecule agents control protein function cellular activity. We develop a computationally efficient network-based method, Ohm, identify characterize communication networks within proteins. Unlike previously developed...

Plants are essential for humans as they serve a source of food, fuel, medicine, oils, and more. The major elements that utilized our needs exist in storage organs, such seeds. These seeds rich proteins, show broad spectrum physiological roles, classified based on their sequence, structure, conserved motifs. With the improvements to knowledge basic sequence structural understanding, we have acquired better insights into seed proteins role. However, still lack systematic analysis towards...

The effect of different concentrations salicylic acid on total Kjeldahl nitrogen and nitrate reductase activity in the maize ( Zea mays L.) seedling was studied. embryonic axis (root + shoot) from seedlings raised with 10 m M Ca(NO 3 ) 2 for 5 days substantially higher than that control when 0.01 supplied. As supply high (1 decreased accumulation organic nitrogen. vivo roots increased at low acid, while were inhibitory. stimulative concentration protected loss under non‐inducing conditions,...

Requirement engineers are not able to elicit and analyze the security requirements clearly, that essential for development of secure reliable software. Proper identification present in Software Specification (SRS) document has been a problem being faced by developers. As result, they deliver software free from threats vulnerabilities. Thus, this paper, we intend mine descriptions SRS thereafter develop classification models. The security-based analyzed using text mining techniques then...

Abstract Forced-copy-choice recombination occurs at the end of a template, differing from copy-choice recombination, which happens internal positions. This mechanism may produce full-length genomes fragments created by host antiviral responses. Previous studies our laboratory demonstrated that poliovirus (PV) RNA-dependent RNA polymerase (RdRp) switches to an “acceptor” template in vitro when initiated on heteropolymeric RNA-primed “donor” template. Surprisingly, recombinants showed...

Biodiversity is structured via complex interactions among ecological, geological, and climatic processes. Regions with high heterogeneity in climate topography are known to harbor fine scale patterns diversity which often overlooked global analyses. Here, we investigate spatial phylogenetic of forest bird across peninsular India, a region topographic heterogeneity. Using comprehensive phylogeny, employ metrics such as Phylogenetic Diversity (PD), Endemism (PE), their relative forms (RPD,...

In this paper, we examine the scope of validity explicit self-organizing map (SOM) magnification control scheme Bauer (1996) on data for which theory does not guarantee success, namely that are n-dimensional, nges2, and whose components in different dimensions statistically independent. The algorithm is very attractive possibility faithful representation probability density function (pdf) a manifold, or discovery rare events, among other properties. Since theoretically unsupported higher...

Defect severity assessment is the most crucial step in large industries and organizations where complexity of software increasing at an exponential rate. Assigning correct level to defects encountered complex software, would help practitioners allocate their resources plan for subsequent defect fixing activities. In order accomplish this, we have developed a model based on text mining techniques that will be used assign each report classification existing reports done using machine learning...

Hippocampal pyramidal neurons are endowed with signature excitability characteristics, exhibit theta-frequency selectivity - manifesting as impedance resonance and a band-pass structure in the spike-triggered average (STA) coincidence detection tuned for gamma-frequency inputs. Are there specific constraints on molecular-scale (ion channel) properties concomitant emergence of cellular-scale encoding (feature selectivity) characteristics? Here, we employed biophysically-constrained unbiased...

Optogenetics is a technique for establishing direct spatiotemporal control over molecular function within living cells using light. Light application induces conformational changes targeted proteins that produce in function. One of the applications optogenetic tools an allosteric via light-sensing domain (LOV2), which allows and robust protein Computational studies supported by cellular imaging demonstrated light allosterically inhibited signaling Vav2, ITSN, Rac1, but structural dynamic...

Abstract Crystal structure of a vicilin, SM80.1, was determined towards exploring its possible physiological functions. The protein purified from Solanum melongena by combination ammonium sulphate fractionation and size exclusion chromatography. Structure ab initio at resolution 1.5 Å X-ray crystallography showing the three-dimensional topology trimeric protein. Each monomer SM80.1 consists two similar domains with hydrophobic binding pocket each accommodating different ligands, i.e. acetate...

A. C. Jain and T. R. Seshadri, Q. Rev. Chem. Soc., 1956, 10, 169 DOI: 10.1039/QR9561000169

Lipids are considered to protect protein allergens from proteolysis and generally seen exist in a bound form. One of the well-known plant families with lipids is non-specific lipid transfer proteins (nsLTPs). Structure-function relationships case members family not clearly understood. As part exploring seed proteome, we have analyzed proteome member Solanaceae family, Solanum melongena (eggplant) S. melongena, SM80.2 was purified, crystallized structure determined at 1.87 Å resolution....

Sequence and structural homology suggests that MP-4 protein from Mucuna pruriens belongs to Kunitz-type protease inhibitor family. However, biochemical assays showed this is a poor of trypsin. To understand the basis observed inhibition, thermodynamics molecular dynamics (MD) simulation studies on binding trypsin were carried out. Molecular simulations revealed temperature influences spectrum conformations adopted by loop regions in structure. At an optimal temperature, achieves maximal...

Class Imbalance Problem is the major issue in machine intelligence producing biased classifiers that work well for majority class but have a relatively poor performance minority class. To ensure development of accurate prediction models, it essential to deal with imbalance problem. In this paper, problem handled using focused undersampling techniques viz. Cluster Based, Tomek Link and Condensed Nearest Neighbours which equalize number instances two types classes by based on some particular...